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Haim Yaffe - One of the best experts on this subject based on the ideXlab platform.

  • comparison between the first and the second electroejaculate qualities obtained from neurologically intact men suffering from anejaculation
    Human Reproduction, 2005
    Co-Authors: Yedidya Hovav, Irit Kafka, Ohn Sibirsky, Rafael N Pollack, Galit Elgavish, Haim Yaffe
    Abstract:

    BACKGROUND: Electroejaculation is an artificial method used to procure semen from neurologically intact men suffering from anejaculation that have failed other treatments. In order to establish the consistency of semen parameters in repeated Electroejaculations, we compared retrospectively the quality of the first and the second electroejaculates of anejaculatory men who were not suffering from any known neurological problems. METHODS: Between 1995 and 2004, 59 neurologically intact men suffering from anejaculation underwent multiple Electroejaculations. Sperm quality of the first and the second ejaculates was compared. RESULTS: A significant difference of 0.33 ± 0.16 ml in the volume of the antegrade portion was found (P = 0.023). The results showed no significant difference in the concentration, motility, count and total motile count of the antegrade ejaculates. In retrograde ejaculates there were no significant differences in the count, motility and total motile count. Neither was there any difference in the total count and the total motile count of both fractions. CONCLUSIONS: Electroejaculation is a reliable method for semen procurement in men suffering from anejaculation. Since semen parameters are consistent, repeated procedures are not justified for improving the sperm quality in anejaculatory, neurologically intact men.

  • short communication comparison between the first and the second electroejaculate qualities obtained from neurologically intact men suffering from anejaculation
    2005
    Co-Authors: Yedidya Hovav, Irit Kafka, Ohn Sibirsky, Rafael N Pollack, Galit Elgavish, Haim Yaffe
    Abstract:

    BACKGROUND: Electroejaculation is an artificial method used to procure semen from neurologically intact men suffering from anejaculation that have failed other treatments. In order to establish the consistency of semen parameters in repeated Electroejaculations, we compared retrospectively the quality of the first and the second electroejaculates of anejaculatory men who were not suffering from any known neurological problems. METHODS: Between 1995 and 2004, 59 neurologically intact men suffering from anejaculation underwent multiple Electroejaculations. Sperm quality of the first and the second ejaculates was compared. RESULTS: A significant difference of 0.33 6 0.16ml in the volume of the antegrade portion was found (P 5 0.023). The results showed no significant difference in the concentration, motility, count and total motile count of the antegrade ejaculates. In retrograde ejaculates there were no significant differences in the count, motility and total motile count. Neither was there any difference in the total count and the total motile count of both fractions. CONCLUSIONS: Electroejaculation is a reliable method for semen procurement in men suffering from anejaculation. Since semen parameters are consistent, repeated procedures are not justified for improving the sperm quality in anejaculatory, neurologically intact men.

  • comparison of semen quality obtained by Electroejaculation and spontaneous ejaculation in men suffering from ejaculation disorder
    Human Reproduction, 2002
    Co-Authors: Yedidya Hovav, Miriam Almagor, Haim Yaffe
    Abstract:

    Background Electroejaculation has become an accepted form of semen procurement in men suffering from anejaculation. However, sperm in these ejaculates often exhibit low motility. The aetiology of asthenozoospermia remains uncertain. It may be related to primary low-grade motility of sperm from anejaculatory men or to detrimental effects of the Electroejaculation process itself. The aim of this study was to determine whether the procedure of Electroejaculation alters the quality of the semen by comparing the characteristics of the electroejaculates with those of the normal ejaculates of the same patients. Methods Nine men suffering from ejaculation disorders underwent 14 procedures of Electroejaculation. The sperm concentration and motility of the 14 samples from Electroejaculation were compared with 20 collected at a later date after successful ejaculation by the same patients. Results The mean +/- SD concentration of the electroejaculates was (52.3 +/- 42.1)x10(6)/ml and of the natural samples (67.4 +/- 38.1)x10(6)/ml. No statistical difference was found between the two groups. The mean percentage of sperm showing progressive motility following Electroejaculation was 23.1 +/- 18.8% and 36.3 +/- 16.7% after spontaneous ejaculation. No statistical difference was found between the groups (P = 0.082). Likewise, the quality of sperm motility was similar after both methods of procurement. Conclusions In our small-sized study group (nine men with ejaculation disorders), the concentration and the motility of sperm obtained by Electroejaculation were not significantly different from sperm obtained naturally.

  • Nocturnal sperm emission in men with psychogenic anejaculation.
    Fertility and sterility, 1999
    Co-Authors: Yedidya Hovav, Mary Dan-goor, Haim Yaffe, Miriam Almagor
    Abstract:

    Abstract Objective: To evaluate sperm characteristics and fertilization potential in sperm obtained from nocturnal emission in men with psychogenic anejaculation. Design: Retrospective study. Setting: In Vitro Fertilization Unit, Bikur Cholim Hospital, Jerusalem, Israel. Patient(s): Six men with psychogenic anejaculation. Intervention(s): Nocturnal emission, Electroejaculation, sperm cryopreservation, and assisted reproduction technologies. Main Outcome Measure(s): Semen analysis, intracytoplasmic sperm injection (ICSI), fertilization rates. Result(s): In four patients, the concentration and motility of sperm obtained from freeze-thawed nocturnal emission were decreased compared with sperm from Electroejaculation. Fertilization rates after ICSI using the nocturnal emission sperm were relatively low (45%). One clinical pregnancy was achieved after intrauterine insemination. Conclusion(s): The quality of sperm from nocturnal emissions is variable, but it can be used in assisted reproduction procedures to avoid aggressive procedures such as Electroejaculation or testis biopsy.

  • Electroejaculation and assisted fertility in men with psychogenic anejaculation
    Fertility and Sterility, 1996
    Co-Authors: Yedidya Hovav, Haim Yaffe, Y Shotland, Miriam Almagor
    Abstract:

    Objectives To evaluate sperm characteristics and fertility potential in ejaculates obtained after Electroejaculation in men with psychogenic anejaculation. Design Retrospective clinical study. Setting In Vitro Fertilization Unit, Bikur Cholim Hospital, Jerusalem, Israel. Patients Twenty men with psychogenic anejaculation who underwent 55 sessions of Electroejaculation and their spouses. Interventions Electroejaculation, assisted reproduction technologies. Main Outcome Measures Semen analysis, IVF, intracytoplasmic injection (ICSI), fertilization rates, and pregnancy rates. Results In all patients, sperm density and motility rates were unsatisfactory (98±127 × 10 6 with 14.6%±15% motility in the antegrade portions and 42±42 X 10 6 with 9.7%±15.6% motility in the retrograde samples). Intrauterine inseminations performed in eight couples did not result in a pregnancy. Four couples underwent IVF-ET treatments. Two pregnancies were achieved with overall success rates of 22% per cycle. Five couples were treated using the ICSI procedure. Although good quality embryos were transferred, none of the treatments resulted in a pregnancy. Conclusions Psychogenic failure to ejaculate may be treated by Electroejaculation. However, the average motility of the sperm obtained is diminished. The combination of Electroejaculation with IVF, including the ICSI procedure, should improve chances of fertilization and pregnancy in these cases.

Yedidya Hovav - One of the best experts on this subject based on the ideXlab platform.

  • comparison between the first and the second electroejaculate qualities obtained from neurologically intact men suffering from anejaculation
    Human Reproduction, 2005
    Co-Authors: Yedidya Hovav, Irit Kafka, Ohn Sibirsky, Rafael N Pollack, Galit Elgavish, Haim Yaffe
    Abstract:

    BACKGROUND: Electroejaculation is an artificial method used to procure semen from neurologically intact men suffering from anejaculation that have failed other treatments. In order to establish the consistency of semen parameters in repeated Electroejaculations, we compared retrospectively the quality of the first and the second electroejaculates of anejaculatory men who were not suffering from any known neurological problems. METHODS: Between 1995 and 2004, 59 neurologically intact men suffering from anejaculation underwent multiple Electroejaculations. Sperm quality of the first and the second ejaculates was compared. RESULTS: A significant difference of 0.33 ± 0.16 ml in the volume of the antegrade portion was found (P = 0.023). The results showed no significant difference in the concentration, motility, count and total motile count of the antegrade ejaculates. In retrograde ejaculates there were no significant differences in the count, motility and total motile count. Neither was there any difference in the total count and the total motile count of both fractions. CONCLUSIONS: Electroejaculation is a reliable method for semen procurement in men suffering from anejaculation. Since semen parameters are consistent, repeated procedures are not justified for improving the sperm quality in anejaculatory, neurologically intact men.

  • short communication comparison between the first and the second electroejaculate qualities obtained from neurologically intact men suffering from anejaculation
    2005
    Co-Authors: Yedidya Hovav, Irit Kafka, Ohn Sibirsky, Rafael N Pollack, Galit Elgavish, Haim Yaffe
    Abstract:

    BACKGROUND: Electroejaculation is an artificial method used to procure semen from neurologically intact men suffering from anejaculation that have failed other treatments. In order to establish the consistency of semen parameters in repeated Electroejaculations, we compared retrospectively the quality of the first and the second electroejaculates of anejaculatory men who were not suffering from any known neurological problems. METHODS: Between 1995 and 2004, 59 neurologically intact men suffering from anejaculation underwent multiple Electroejaculations. Sperm quality of the first and the second ejaculates was compared. RESULTS: A significant difference of 0.33 6 0.16ml in the volume of the antegrade portion was found (P 5 0.023). The results showed no significant difference in the concentration, motility, count and total motile count of the antegrade ejaculates. In retrograde ejaculates there were no significant differences in the count, motility and total motile count. Neither was there any difference in the total count and the total motile count of both fractions. CONCLUSIONS: Electroejaculation is a reliable method for semen procurement in men suffering from anejaculation. Since semen parameters are consistent, repeated procedures are not justified for improving the sperm quality in anejaculatory, neurologically intact men.

  • comparison of semen quality obtained by Electroejaculation and spontaneous ejaculation in men suffering from ejaculation disorder
    Human Reproduction, 2002
    Co-Authors: Yedidya Hovav, Miriam Almagor, Haim Yaffe
    Abstract:

    Background Electroejaculation has become an accepted form of semen procurement in men suffering from anejaculation. However, sperm in these ejaculates often exhibit low motility. The aetiology of asthenozoospermia remains uncertain. It may be related to primary low-grade motility of sperm from anejaculatory men or to detrimental effects of the Electroejaculation process itself. The aim of this study was to determine whether the procedure of Electroejaculation alters the quality of the semen by comparing the characteristics of the electroejaculates with those of the normal ejaculates of the same patients. Methods Nine men suffering from ejaculation disorders underwent 14 procedures of Electroejaculation. The sperm concentration and motility of the 14 samples from Electroejaculation were compared with 20 collected at a later date after successful ejaculation by the same patients. Results The mean +/- SD concentration of the electroejaculates was (52.3 +/- 42.1)x10(6)/ml and of the natural samples (67.4 +/- 38.1)x10(6)/ml. No statistical difference was found between the two groups. The mean percentage of sperm showing progressive motility following Electroejaculation was 23.1 +/- 18.8% and 36.3 +/- 16.7% after spontaneous ejaculation. No statistical difference was found between the groups (P = 0.082). Likewise, the quality of sperm motility was similar after both methods of procurement. Conclusions In our small-sized study group (nine men with ejaculation disorders), the concentration and the motility of sperm obtained by Electroejaculation were not significantly different from sperm obtained naturally.

  • Nocturnal sperm emission in men with psychogenic anejaculation.
    Fertility and sterility, 1999
    Co-Authors: Yedidya Hovav, Mary Dan-goor, Haim Yaffe, Miriam Almagor
    Abstract:

    Abstract Objective: To evaluate sperm characteristics and fertilization potential in sperm obtained from nocturnal emission in men with psychogenic anejaculation. Design: Retrospective study. Setting: In Vitro Fertilization Unit, Bikur Cholim Hospital, Jerusalem, Israel. Patient(s): Six men with psychogenic anejaculation. Intervention(s): Nocturnal emission, Electroejaculation, sperm cryopreservation, and assisted reproduction technologies. Main Outcome Measure(s): Semen analysis, intracytoplasmic sperm injection (ICSI), fertilization rates. Result(s): In four patients, the concentration and motility of sperm obtained from freeze-thawed nocturnal emission were decreased compared with sperm from Electroejaculation. Fertilization rates after ICSI using the nocturnal emission sperm were relatively low (45%). One clinical pregnancy was achieved after intrauterine insemination. Conclusion(s): The quality of sperm from nocturnal emissions is variable, but it can be used in assisted reproduction procedures to avoid aggressive procedures such as Electroejaculation or testis biopsy.

  • antisperm antibodies in men with psychogenic anejaculation
    Archives of Andrology, 1998
    Co-Authors: Miriam Almagor, Yedidya Hovav, Mary Dangoor, Irit Kafka, Y Shotland
    Abstract:

    Antisperm autoantibodies were determined in 16 men suffering from psychogenic anejaculation who underwent assisted reproduction treatments. Blood and semen samples were collected after transrectal Electroejaculation and antisperm antibodies in serum and on the surface of motile spermatozoa were measured using the direct and indirect immunobead binding test. Five men (31%) were found positive for antisperm antibodies. The majority of antibodies were directed against the sperm heads. Surface antibodies were mainly IgA isotype whereas serum antibodies were IgG isotype. These results suggest that psychogenic anejaculation might be associated with increased incidence of antisperm autoimmunity.

Miriam Almagor - One of the best experts on this subject based on the ideXlab platform.

  • comparison of semen quality obtained by Electroejaculation and spontaneous ejaculation in men suffering from ejaculation disorder
    Human Reproduction, 2002
    Co-Authors: Yedidya Hovav, Miriam Almagor, Haim Yaffe
    Abstract:

    Background Electroejaculation has become an accepted form of semen procurement in men suffering from anejaculation. However, sperm in these ejaculates often exhibit low motility. The aetiology of asthenozoospermia remains uncertain. It may be related to primary low-grade motility of sperm from anejaculatory men or to detrimental effects of the Electroejaculation process itself. The aim of this study was to determine whether the procedure of Electroejaculation alters the quality of the semen by comparing the characteristics of the electroejaculates with those of the normal ejaculates of the same patients. Methods Nine men suffering from ejaculation disorders underwent 14 procedures of Electroejaculation. The sperm concentration and motility of the 14 samples from Electroejaculation were compared with 20 collected at a later date after successful ejaculation by the same patients. Results The mean +/- SD concentration of the electroejaculates was (52.3 +/- 42.1)x10(6)/ml and of the natural samples (67.4 +/- 38.1)x10(6)/ml. No statistical difference was found between the two groups. The mean percentage of sperm showing progressive motility following Electroejaculation was 23.1 +/- 18.8% and 36.3 +/- 16.7% after spontaneous ejaculation. No statistical difference was found between the groups (P = 0.082). Likewise, the quality of sperm motility was similar after both methods of procurement. Conclusions In our small-sized study group (nine men with ejaculation disorders), the concentration and the motility of sperm obtained by Electroejaculation were not significantly different from sperm obtained naturally.

  • Nocturnal sperm emission in men with psychogenic anejaculation.
    Fertility and sterility, 1999
    Co-Authors: Yedidya Hovav, Mary Dan-goor, Haim Yaffe, Miriam Almagor
    Abstract:

    Abstract Objective: To evaluate sperm characteristics and fertilization potential in sperm obtained from nocturnal emission in men with psychogenic anejaculation. Design: Retrospective study. Setting: In Vitro Fertilization Unit, Bikur Cholim Hospital, Jerusalem, Israel. Patient(s): Six men with psychogenic anejaculation. Intervention(s): Nocturnal emission, Electroejaculation, sperm cryopreservation, and assisted reproduction technologies. Main Outcome Measure(s): Semen analysis, intracytoplasmic sperm injection (ICSI), fertilization rates. Result(s): In four patients, the concentration and motility of sperm obtained from freeze-thawed nocturnal emission were decreased compared with sperm from Electroejaculation. Fertilization rates after ICSI using the nocturnal emission sperm were relatively low (45%). One clinical pregnancy was achieved after intrauterine insemination. Conclusion(s): The quality of sperm from nocturnal emissions is variable, but it can be used in assisted reproduction procedures to avoid aggressive procedures such as Electroejaculation or testis biopsy.

  • antisperm antibodies in men with psychogenic anejaculation
    Archives of Andrology, 1998
    Co-Authors: Miriam Almagor, Yedidya Hovav, Mary Dangoor, Irit Kafka, Y Shotland
    Abstract:

    Antisperm autoantibodies were determined in 16 men suffering from psychogenic anejaculation who underwent assisted reproduction treatments. Blood and semen samples were collected after transrectal Electroejaculation and antisperm antibodies in serum and on the surface of motile spermatozoa were measured using the direct and indirect immunobead binding test. Five men (31%) were found positive for antisperm antibodies. The majority of antibodies were directed against the sperm heads. Surface antibodies were mainly IgA isotype whereas serum antibodies were IgG isotype. These results suggest that psychogenic anejaculation might be associated with increased incidence of antisperm autoimmunity.

  • Electroejaculation in combination with intracytoplasmic sperm injection in patients with psychogenic anejaculation results in lower fertilization rates
    Fertility and Sterility, 1998
    Co-Authors: M Yedidya D Hovav, Mary Dangoor, M Irit D Kafka, M Haim D Yaffe, Miriam Almagor
    Abstract:

    Abstract Objective: To evaluate the outcome of intracytoplasmic sperm injection (ICSI) with sperm obtained by Electroejaculation in men with psychogenic anejaculation. Design: Retrospective clinical study. Setting: In Vitro Fertilization Unit, Bikur Cholim Hospital, Jerusalem, Israel. Patient(s): Seven men with psychogenic anejaculation who underwent 16 sessions of Electroejaculation in combination with ICSI. Intervention(s): Electroejaculation, ICSI. Main Outcome Measure(s): Semen analysis, ICSI, fertilization rates. Result(s): All patients had poor sperm motility. One hundred forty-seven oocytes were injected, with a fertilization rate of 27% (39/142). One ongoing pregnancy was achieved. Conclusion(s): Sperm obtained by Electroejaculation have low motility and reduced fertilization potential. Nevertheless, ICSI should be offered to improve the possibility of successful pregnancy.

  • Electroejaculation and assisted fertility in men with psychogenic anejaculation
    Fertility and Sterility, 1996
    Co-Authors: Yedidya Hovav, Haim Yaffe, Y Shotland, Miriam Almagor
    Abstract:

    Objectives To evaluate sperm characteristics and fertility potential in ejaculates obtained after Electroejaculation in men with psychogenic anejaculation. Design Retrospective clinical study. Setting In Vitro Fertilization Unit, Bikur Cholim Hospital, Jerusalem, Israel. Patients Twenty men with psychogenic anejaculation who underwent 55 sessions of Electroejaculation and their spouses. Interventions Electroejaculation, assisted reproduction technologies. Main Outcome Measures Semen analysis, IVF, intracytoplasmic injection (ICSI), fertilization rates, and pregnancy rates. Results In all patients, sperm density and motility rates were unsatisfactory (98±127 × 10 6 with 14.6%±15% motility in the antegrade portions and 42±42 X 10 6 with 9.7%±15.6% motility in the retrograde samples). Intrauterine inseminations performed in eight couples did not result in a pregnancy. Four couples underwent IVF-ET treatments. Two pregnancies were achieved with overall success rates of 22% per cycle. Five couples were treated using the ICSI procedure. Although good quality embryos were transferred, none of the treatments resulted in a pregnancy. Conclusions Psychogenic failure to ejaculate may be treated by Electroejaculation. However, the average motility of the sperm obtained is diminished. The combination of Electroejaculation with IVF, including the ICSI procedure, should improve chances of fertilization and pregnancy in these cases.

J Santiagomoreno - One of the best experts on this subject based on the ideXlab platform.

  • using transrectal ultrasound guided massage of the accessory sex glands for buck semen collection yields semen with greater cryoresistance than Electroejaculation alone during the breeding season
    Theriogenology, 2021
    Co-Authors: Madeleine Guerrerogutierrez, J Santiagomoreno, Rodolfo Ungerfeld, Mariana Garcia Kako Rodriguez, Julia Giriboni
    Abstract:

    Abstract The cryoresistance of bucks’ semen collected by transrectal ultrasound-guided massage of the accessory sex glands (TUMASG) and Electroejaculation (EE) was compared during the breeding season (Study 1) or the late non-breeding season (Study 2). Semen was collected from 10 Gabon bucks with both methods in each season and then frozen according to a standardized protocol. The time required for ejaculation, the number of electrical pulses applied, the sum of the electrical pulses applied∗voltage and the total number of vocalizations emitted during the collection by each animal were recorded. In Study 1, TUMASG required a longer time (P = 0.0006) but fewer electrical pulses and a lower sum of pulses∗voltage than EE (P

  • sperm collection by transrectal ultrasound guided massage of the accessory sex glands is less stressful than Electroejaculation without altering sperm characteristics in conscious goat bucks
    Theriogenology, 2017
    Co-Authors: S Abrilsanchez, J Santiagomoreno, Julia Giriboni, Florencia Beracochea, Aline Freitasdemelo, Juan Pablo Damian, Rodolfo Ungerfeld
    Abstract:

    Abstract In anesthetized non-domestic ruminants transrectal ultrasound-guided massage of the accessory sex glands (TUMASG) is an alternative method to collect semen slightly less stressful than Electroejaculation (EE). However, some sperm characteristics are better when semen is collected with EE than with TUMASG. As anesthesia reduces the response to stressors, the advantages of TUMASG may be reduced in anesthetized animals, and thus, TUMASG may be even more advantageous in conscious animals. Therefore, the aim of the present study was to compare the stress response and the characteristics of the sperm collected with TUMASG and EE in conscious goat bucks. Semen was collected in 10 bucks with both procedures. During each procedure, the time required for ejaculation, the number of electric pulses applied and the number of vocalizations were recorded. Rectal temperature, heart rate, serum cortisol concentration, biochemical and hematological parameters were measured before and after each procedure. Sperm characteristics [ejaculated volume, sperm concentration, sperm mass motility (scale 0–5), sperm vigor (scale 0–5), the percentages of motile and progressive motile sperm, of sperm vitality, of sperm with plasma membrane integrity, and with acrosome damage and morphological abnormalities] were also determined. Electroejaculation required more electric pulses than TUMASG (P

  • oxytocin administration before sperm collection by transrectal ultrasonic guided massage of the accessory sex glands in mouflons and bucks
    Animal Reproduction Science, 2016
    Co-Authors: Rodolfo Ungerfeld, A Toledanodiaz, C Castano, Julia Giriboni, S Abrilsanchez, Florencia Beracochea, J Santiagomoreno
    Abstract:

    Transrectal ultrasonic-guided massage of the accessory sex glands (TUMASG) is an alternative technique for sperm collection which decreases negative animal welfare concerns of Electroejaculation. However, quality of collected sperm with TUMASG is less than that collected by Electroejaculation. Utilisation of TUMASG may not, however, induce strong muscular contractions, so administration of oxytocin before the procedure may improve sperm quality. Therefore, two studies were designed to determine if administration of oxytocin before TUMASG improves the procedure and quality of the collected semen. The first study was performed with anaesthetised mouflons, and the second with non-anaesthetised Gabon bucks. Time length, number of electric pulses applied and numbers of vocalizations were recorded, and sperm characteristics were analysed. The number of electrical pulses and sperm characteristics did not differ with oxytocin administration in mouflons. However, oxytocin administration decreased time length (P=0.02) and tended to decrease the number of electric pulses needed for induction of ejaculation (P=0.08) in bucks. The number of vocalizations and sperm variables did not differ when the two procedures were used. In conclusion, oxytocin administration before TUMASG shortened the procedure and tended to decrease the number of electrical pulses needed to induce ejaculation in conscious bucks, thus, decreasing welfare concerns for the animals in which TUMASG is used. Therefore, its routine inclusion in this species should be considered.

  • giant panda ailuropoda melanoleuca sperm morphometry and function after repeated freezing and thawing
    Andrologia, 2016
    Co-Authors: J Santiagomoreno, J Pradiee, E Obrien, Eva Martineznevado, M Delclaux, Cristina Castaño, M C Esteso, A Toledanodiaz, A Lopezsebastian, J Fernandezmoran
    Abstract:

    Summary This work examines the effects of subsequent cycles of freezing–thawing on giant panda (Ailuropoda melanoleuca) sperm morphometry and function, and assesses whether density-gradient centrifugation (DGC) can increase the number of freezing–thawing cycles this sperm can withstand. A sperm sample was collected by Electroejaculation from a mature giant panda and subjected to five freezing–thawing cycles. Although repeated freezing–thawing negatively affected (P  60% of the sperm cells in both treatments showed acrosome integrity even after the fifth freezing cycle. In fresh semen, the sperm head length was 4.7 μm, the head width 3.6 μm, area 14.3 μm2 and perimeter length 14.1 μm. The present results suggest that giant panda sperm trends to be resistant to repeated freezing–thawing, even without DGC selection.

  • physiological responses and characteristics of sperm collected after Electroejaculation or transrectal ultrasound guided massage of the accessory sex glands in anesthetized mouflons ovis musimon and iberian ibexes capra pyrenaica
    Theriogenology, 2015
    Co-Authors: Rodolfo Ungerfeld, J Pradiee, M C Esteso, A Toledanodiaz, A Lopezsebastian, C Castano, Beatriz Labrador, J Santiagomoreno
    Abstract:

    The objective was to characterize the stress response and the seminal parameters obtained with Electroejaculation (EE) or transrectal ultrasound-guided massage of the accessory sex glands (TUMASG) in two captive but nondomestic ruminants, the mouflons and the Iberian ibex under general anesthesia. In mouflons, the physiological responses (heart and respiratory rate, rectal temperature, cortisol, creatine kinase, potassium and glucose concentrations) changed similarly with both procedures. The TUMASG procedure was faster than EE in mouflons (21.7 ± 1.4 vs. 12.4 ± 1.2 minutes, P < 0.01). In ibexes, respiratory rate, cortisol and creatine kinase concentration changes were greater with EE than with TUMASG (final respiratory rate: 62.7 ± 5.5 vs. 38.1 ± 5.6 breaths/min [P < 0.05]; final cortisol: 51.4 ± 5.1 vs. 25.3 ± 5.6 ng/mL [P < 0.001]; and final creatine kinase: 300.9 ± 99.9 vs. 87.1 ± 16.9 U/L [P < 0.001]). Electroejaculation provided better results in some sperm parameters (mouflons: sperm score: 3.4 ± 0.3 vs. 2.6 ± 0.2 [P < 0.01]; total number of sperm ejaculated: 982.4 ± 299 vs. 710.0 ± 542.2 [P < 0.05]; ibexes: sperm with progressive motility: 47.7 ± 6.2 vs. 20.5 ± 8.3 [P < 0.05]). The transrectal ultrasound-guided massage of the accessory sex glands appears to be an alternative technique to collect sperm from wild ruminants, reducing the need for electrical stimuli and thus decreasing the undesired responses of EE in the more sensitive species. On the other hand, better fresh sperm may be collected with EE. However, TUMASG provides practical advantages in animal welfare, firstly in these wild species more sensible to stress management and capture myopathy.

A Lopezsebastian - One of the best experts on this subject based on the ideXlab platform.

  • giant panda ailuropoda melanoleuca sperm morphometry and function after repeated freezing and thawing
    Andrologia, 2016
    Co-Authors: J Santiagomoreno, J Pradiee, E Obrien, Eva Martineznevado, M Delclaux, Cristina Castaño, M C Esteso, A Toledanodiaz, A Lopezsebastian, J Fernandezmoran
    Abstract:

    Summary This work examines the effects of subsequent cycles of freezing–thawing on giant panda (Ailuropoda melanoleuca) sperm morphometry and function, and assesses whether density-gradient centrifugation (DGC) can increase the number of freezing–thawing cycles this sperm can withstand. A sperm sample was collected by Electroejaculation from a mature giant panda and subjected to five freezing–thawing cycles. Although repeated freezing–thawing negatively affected (P  60% of the sperm cells in both treatments showed acrosome integrity even after the fifth freezing cycle. In fresh semen, the sperm head length was 4.7 μm, the head width 3.6 μm, area 14.3 μm2 and perimeter length 14.1 μm. The present results suggest that giant panda sperm trends to be resistant to repeated freezing–thawing, even without DGC selection.

  • physiological responses and characteristics of sperm collected after Electroejaculation or transrectal ultrasound guided massage of the accessory sex glands in anesthetized mouflons ovis musimon and iberian ibexes capra pyrenaica
    Theriogenology, 2015
    Co-Authors: Rodolfo Ungerfeld, J Pradiee, M C Esteso, A Toledanodiaz, A Lopezsebastian, C Castano, Beatriz Labrador, J Santiagomoreno
    Abstract:

    The objective was to characterize the stress response and the seminal parameters obtained with Electroejaculation (EE) or transrectal ultrasound-guided massage of the accessory sex glands (TUMASG) in two captive but nondomestic ruminants, the mouflons and the Iberian ibex under general anesthesia. In mouflons, the physiological responses (heart and respiratory rate, rectal temperature, cortisol, creatine kinase, potassium and glucose concentrations) changed similarly with both procedures. The TUMASG procedure was faster than EE in mouflons (21.7 ± 1.4 vs. 12.4 ± 1.2 minutes, P < 0.01). In ibexes, respiratory rate, cortisol and creatine kinase concentration changes were greater with EE than with TUMASG (final respiratory rate: 62.7 ± 5.5 vs. 38.1 ± 5.6 breaths/min [P < 0.05]; final cortisol: 51.4 ± 5.1 vs. 25.3 ± 5.6 ng/mL [P < 0.001]; and final creatine kinase: 300.9 ± 99.9 vs. 87.1 ± 16.9 U/L [P < 0.001]). Electroejaculation provided better results in some sperm parameters (mouflons: sperm score: 3.4 ± 0.3 vs. 2.6 ± 0.2 [P < 0.01]; total number of sperm ejaculated: 982.4 ± 299 vs. 710.0 ± 542.2 [P < 0.05]; ibexes: sperm with progressive motility: 47.7 ± 6.2 vs. 20.5 ± 8.3 [P < 0.05]). The transrectal ultrasound-guided massage of the accessory sex glands appears to be an alternative technique to collect sperm from wild ruminants, reducing the need for electrical stimuli and thus decreasing the undesired responses of EE in the more sensitive species. On the other hand, better fresh sperm may be collected with EE. However, TUMASG provides practical advantages in animal welfare, firstly in these wild species more sensible to stress management and capture myopathy.

  • cryopreservation of aoudad ammotragus lervia sahariensis sperm obtained by transrectal ultrasound guided massage of the accessory sex glands and Electroejaculation
    Theriogenology, 2013
    Co-Authors: J Santiagomoreno, M C Esteso, A Toledanodiaz, A Lopezsebastian, C Castano, R Guerra, M J Ruiz, N Mendoza, C Luna, J A Cebrianperez
    Abstract:

    This study examines (1) the effectiveness of transrectal, ultrasound-guided massage of the accessory sex glands (TUMASG) combined with Electroejaculation for obtaining aoudad (Ammotragus lervia sahariensis) sperm samples for cryopreservation, and (2) the effectiveness of a Tris-citric acid–glucose-based medium (TCG; usually used for freezing ibex sperm) and a TES-Tris–glucose-based medium (TTG; typically used in the cryopreservation of mouflon sperm) as sperm extenders. After TUMASG, just one to three electrical pulses were required for ejaculation to occur in five of the six animals studied; one ejaculated after TUMASG alone. Transrectal, ultrasound-guided massage of the accessory sex glands would therefore appear to be useful in obtaining sperm samples from this species, requiring few subsequent electrical Electroejaculation stimuli and sometimes none at all. After thawing, the membrane integrity (assessed by nigrosin-eosin staining) of sperm extended with TTG was greater than that of sperm extended with TCG (P < 0.05). The total percentage of sperm showing an intact acrosome, as assessed by fluorescein isothiocyanate-conjugated peanut (Arachis hypogea) agglutinin, was also higher in the TTG-extended sperm (P < 0.05), and the percentage of dead sperm with a damaged acrosome was lower (P < 0.05). No differences were seen between TCG and TTG in terms of apoptotic manifestations (DNA damage, caspase activity, mitochondrial membrane potential, and plasmalemma stability). Therefore, TTG appears to be a better extender than TCG for cryopreserving aoudad sperm.

  • effects of anesthetic protocols on Electroejaculation variables of iberian ibex capra pyrenaica
    Research in Veterinary Science, 2011
    Co-Authors: J Santiagomoreno, A Toledanodiaz, A Sookhthezary, Felix Gomezguillamon, Salas R De La Vega, Antonio Pulidopastor, A Lopezsebastian
    Abstract:

    Abstract The effects of three intramuscular anesthesia protocols – detomidine 190 μg/kg plus ketamine 2 mg/kg, detomidine 270 μg/kg plus ketamine 1.4 mg/kg, and tiletamine 3.4 mg/kg plus zolazepam 3.4 mg/kg – on penis protrusion and ejaculation variables were compared in nine captive Spanish ibex ( Capra pyrenaica ) subjected to Electroejaculation. Body temperature, heart, and respiratory rates, as well as a number of plasma biochemical variables were also recorded prior to and during anesthesia. The detomidine plus ketamine protocols induced bradycardia and increased respiratory rate. However, the tiletamine/zolazepam protocol did not affect heart and respiratory rates. None of the three protocols caused a substantial change in rectal temperature, yet all protocols caused a significant increase in plasma glucose levels. Differences in anesthetic protocols did not affect sperm quality or quantity. However, choice of anesthetic protocol affected ( P

  • cryopreservation of spanish ibex capra pyrenaica sperm obtained by Electroejaculation outside the rutting season
    Theriogenology, 2009
    Co-Authors: J Santiagomoreno, Felix Gomezguillamon, Antonio Pulidopastor, Miguel Angel Coloma, J Dorado, Ricardo Salasvega, A Gomezbrunet, A Lopezsebastian
    Abstract:

    Abstract The effectiveness of Electroejaculation for obtaining Spanish ibex sperm samples for freeze preserving outside the rutting season was evaluated—the aim being to optimise biological resources for the establishment of germplasm banks. The effect of different egg yolk concentrations (6% or 12%, v/v) in diluents of different buffer composition (Tris–citric acid buffer or Tes–Tris buffer) on frozen-thawed samples of the above also investigated. Experiments were undertaken with six ibex males in February–May, and involved four different semen samples from each animal with four combination of extender, respectively: Tes–Tris–glucose (TTG)–6% egg yolk, TTG–12% egg yolk, Tris–citric acid–glucose (TCG)–12% egg yolk, TCG–6% egg yolk. The results show that Electroejaculation is a useful way of obtaining sperm samples from Spanish ibex outside the rutting season (i.e., at a time coinciding with plasma testosterone levels of