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Pewpan M. Intapan - One of the best experts on this subject based on the ideXlab platform.
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Development and usefulness of an immunochromatographic device to detect antibodies for rapid diagnosis of human Gnathostomiasis
Parasites & vectors, 2016Co-Authors: Penchom Janwan, Porntip Laummaunwai, Pewpan M. Intapan, Oranuch Sanpool, Rutchanee Rodpai, Tongjit Thanchomnang, Hiroshi Yamasaki, Katsuyoshi Takayama, Kobayashi Kaoru, Yukuharu KobayashiAbstract:Background Human Gnathostomiasis is a serious tropical disease, which is often overlooked. There is an urgent need to improve tools to aid the potential diagnosis of the disease in endemic regions. To overcome this, we produced the immunochromatographic test (ICT) kit for a rapid and simple diagnosis of human Gnathostomiasis.
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Development and usefulness of an immunochromatographic device to detect antibodies for rapid diagnosis of human Gnathostomiasis
Parasites & Vectors, 2016Co-Authors: Penchom Janwan, Porntip Laummaunwai, Pewpan M. Intapan, Oranuch Sanpool, Rutchanee Rodpai, Tongjit Thanchomnang, Hiroshi Yamasaki, Kaoru Kobayashi, Katsuyoshi Takayama, Yukuharu KobayashiAbstract:Background Human Gnathostomiasis is a serious tropical disease, which is often overlooked. There is an urgent need to improve tools to aid the potential diagnosis of the disease in endemic regions. To overcome this, we produced the immunochromatographic test (ICT) kit for a rapid and simple diagnosis of human Gnathostomiasis. Findings The recombinant protein (named rGslic18) was applied to ICT kit as the antigen. The diagnostic value of ICT kit was evaluated using serum samples from parasitologically proven and clinically suspected Gnathostomiasis patients, healthy volunteers and patients with other parasitic diseases. The ICT kit exhibited quite high sensitivity (93.75 %) and specificity (97.01 %). Conclusions The ICT kit is simple, convenient and easy to implement and expected to provide reliable diagnostic results for human Gnathostomiasis. It also will be a promising diagnostic tool not only for large-scale epidemiological surveys in endemic or remote areas where diagnostic facilities are poor but also for a rapid clinical diagnosis in the bedside laboratory.
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proteomic analysis identification of antigenic proteins in gnathostoma spinigerum larvae
Experimental Parasitology, 2015Co-Authors: Tonkla Insawang, Porntip Laummaunwai, Chaisiri Wongkham, Pewpan M. Intapan, Rutchanee Rodpai, Tongjit Thanchomnang, Penchom Janwan, Oranuch SanpoolAbstract:Abstract Gnathostoma spinigerum is the causative agent of human Gnathostomiasis. The advanced third stage larva (AL3) of this nematode can migrate into the subcutaneous tissues, including vital organs, often producing severe pathological effects. This study performed immuno-proteomic analysis of antigenic spots, derived from G. spinigerum advanced third stage larva (GSAL3) and recognized by human Gnathostomiasis sera, using two-dimensional (2-DE) gel electrophoresis based-liquid chromatography/tandem mass spectrometry (LC/MS–MS), and followed by the aid of a database search. The crude GSAL3 extract was fractionated using IPG strips (pH 3-11NL) and followed by SDS-PAGE in the second dimension. Each gel was stained with colloidal Coomassie blue or was electro-transferred onto a nitrocellulose membrane and probed with Gnathostomiasis human sera by immunoblotting. Individual Coomassie-stained protein spots corresponding to the antigenic spots recognized by immunoblotting were excised and processed using LC/MS–MS. Of the 93 antigenic spots excised, 87 were identified by LC/MS–MS. Twenty-seven protein types were found, the most abundant being Ascaris suum37. Six spots showed good quality spectra, but could not be identified. This appears to be the first attempt to characterize antigenic proteins from GSAL3 using a proteomic approach. Immuno-proteomics shows promise to assist the search for candidate proteins for diagnosis and vaccine/drug design and may provide better understand of the host-parasite relationship in human Gnathostomiasis.
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ehmti 0025 clinical manifestations of subarachnoid hemorrhage from gnathostoma spinigerum in srinagarind hospital
Journal of Headache and Pain, 2014Co-Authors: Kittisak Sawanyawisuth, Jaturat Kanpittaya, Verajit Chotmongkol, Keetapong Pongtipakorn, Kannikar Kongbunkiat, Panita Limpawattana, Vichai Senthong, Jarin Chindaprasirt, W Waranon, Pewpan M. IntapanAbstract:Subarachnoid hemorrhage (SAH) is a serious neurological condition. Common cause of SAH is vascular origin. Gnathostomiasis is also a common disease in Thailand and may cause SAH.
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SUBARACHNOID HEMORRHAGE DUE TO VASCULAR CAUSES AND Gnathostomiasis: CLINICAL FEATURES AND LABORATORY FINDINGS
2014Co-Authors: Waranon Munkong, Jaturat Kanpittaya, Verajit Chotmongkol, Kittisak Sawanyawisuth, Keetapong Pongtipakorn, Kannikar Kongbunkiat, Panita Limpawattana, Vichai Senthong, Jarin Chindaprasirt, Pewpan M. IntapanAbstract:Subarachnoid hemorrhage (SAH) is a serious neurological condition, commonly of vascular etiology. Gnathostomiasis is a common parasitic disease in Thailand and may also cause SAH. The purpose of this study was to find clini - cal differences between SAH due to these two causes. This was a retrospective study and collected data from medical charts of patients diagnosed with SAH at Srinagarind Hospital, Khon Kaen, during 2009 and 2011. SAH due to vascular causes was diagnosed by cerebral angiogram, while cerebral Gnathostomiasis, in which cerebral angiograms were negative, was diagnosed immunologically. Differences in clinical features between the two groups were compared using de - scriptive statistics. Eighteen patients had SAH due to vascular causes and ten had Gnathostomiasis. Most parameters were similar between the two groups. However, the cerebrospinal fluid glucose /plasma glucose ratio in the Gnathostomiasis group was significantly higher than in the vascular group (80% vs 16.67%, respectively). In conclusion, cerebrospinal fluid glucose /plasma glucose ratio was significantly higher in SAH patients caused by Gnathostomiasis than vascular group and may provide a diagnostic tool for distinguishing between these two etiologies.
Kittisak Sawanyawisuth - One of the best experts on this subject based on the ideXlab platform.
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Ocular Gnathostomiasis-Update of Earlier Survey.
The American journal of tropical medicine and hygiene, 2017Co-Authors: Yukifumi Nawa, Verajit Chotmongkol, Kittisak Sawanyawisuth, Masahide Yoshikawa, Sandra Fernandez Figueiras, Maria D Benavides, Sylvia Páz Díaz CamachoAbstract:Ocular Gnathostomiasis is rather a rare food-borne zoonosis caused by infection with the larvae of several species of genus Gnathostoma and is a representative ocular larva migrans syndrome. In our previous literature survey, we found 73 cases of ocular Gnathostomiasis reported up to and including 2009, though additional sporadic cases have been reported in Asia and the Americas since that report. Here, we review 10 additional cases reported since 2010, and also update current findings regarding epidemiological and clinical features in affected patients.
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MR Imaging Findings in Cerebrospinal
2015Co-Authors: Kittisak Sawanyawisuth, Jaturat Kanpittaya, Paron Dekumyoy, Somsak Tiamkao, Suthipun JitpimolmardAbstract:Summary: Human Gnathostomiasis is an infection caused mainly by Gnathostoma spinigerum, a nematode. Infected humans can present with various clinical manifestations. Serology is the criterion standard for diagnosing gnathos-tomiasis, whereas MR imaging represents a complemen-tary tool for assessing severity and extent of disease. We report two definite cases of Gnathostomiasis that were con-firmed by the immunoblotting technique. MR imaging of the cervical cords showed cord enlargement and diffuse high signal intensity, mainly of the gray-white matter re-gions. MR imaging of the brain showed hemorrhagic tract and scattered deep intracerebral hemorrhage with diffuse, fuzzy white matter lesions with nodular enhancement. Se-vere Gnathostomiasis was unresponsive to treatment. In northeast Thailand, consumption of raw fish, snake, or other intermediate hosts, such as chicken and duck, has made infection with Gnathostoma spini-gerum a common cause of asymmetrical paraparesis with eosinophilic CSF (1). Infection can also occur from contact with parasite-infested meats, unwashed utensils, and prenatal transmission. After exiting the intestine, the parasite may present as migratory swell-ing, ocular Gnathostomiasis, radiculitis, myelitis, sub-arachnoid hemorrhage, and/or intracerebral hemor-rhage. Clinical manifestations might involve one or several organs, with or without disease progression, depending on the route taken by the parasite. MR imaging of the cervical cord in Gnathostomiasis was first reported in France (2), in a case in which a patient presented with a cord lesion and MR imaging was helpful in diagnosing and assessing the severity of disease. Here we report the MR findings of two cases of clinically diagnosed Gnathostomiasis that were con-firmed by serology
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ehmti 0025 clinical manifestations of subarachnoid hemorrhage from gnathostoma spinigerum in srinagarind hospital
Journal of Headache and Pain, 2014Co-Authors: Kittisak Sawanyawisuth, Jaturat Kanpittaya, Verajit Chotmongkol, Keetapong Pongtipakorn, Kannikar Kongbunkiat, Panita Limpawattana, Vichai Senthong, Jarin Chindaprasirt, W Waranon, Pewpan M. IntapanAbstract:Subarachnoid hemorrhage (SAH) is a serious neurological condition. Common cause of SAH is vascular origin. Gnathostomiasis is also a common disease in Thailand and may cause SAH.
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SUBARACHNOID HEMORRHAGE DUE TO VASCULAR CAUSES AND Gnathostomiasis: CLINICAL FEATURES AND LABORATORY FINDINGS
2014Co-Authors: Waranon Munkong, Jaturat Kanpittaya, Verajit Chotmongkol, Kittisak Sawanyawisuth, Keetapong Pongtipakorn, Kannikar Kongbunkiat, Panita Limpawattana, Vichai Senthong, Jarin Chindaprasirt, Pewpan M. IntapanAbstract:Subarachnoid hemorrhage (SAH) is a serious neurological condition, commonly of vascular etiology. Gnathostomiasis is a common parasitic disease in Thailand and may also cause SAH. The purpose of this study was to find clini - cal differences between SAH due to these two causes. This was a retrospective study and collected data from medical charts of patients diagnosed with SAH at Srinagarind Hospital, Khon Kaen, during 2009 and 2011. SAH due to vascular causes was diagnosed by cerebral angiogram, while cerebral Gnathostomiasis, in which cerebral angiograms were negative, was diagnosed immunologically. Differences in clinical features between the two groups were compared using de - scriptive statistics. Eighteen patients had SAH due to vascular causes and ten had Gnathostomiasis. Most parameters were similar between the two groups. However, the cerebrospinal fluid glucose /plasma glucose ratio in the Gnathostomiasis group was significantly higher than in the vascular group (80% vs 16.67%, respectively). In conclusion, cerebrospinal fluid glucose /plasma glucose ratio was significantly higher in SAH patients caused by Gnathostomiasis than vascular group and may provide a diagnostic tool for distinguishing between these two etiologies.
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application of recombinant gnathostoma spinigerum matrix metalloproteinase like protein for serodiagnosis of human Gnathostomiasis by immunoblotting
American Journal of Tropical Medicine and Hygiene, 2013Co-Authors: Penchom Janwan, Porntip Laummaunwai, Chaisiri Wongkham, Pewpan M. Intapan, Viraphong Lulitanond, Chatchai Tayapiwatana, Hiroshi Yamasaki, Amnat Kitkhuandee, Kittisak Sawanyawisuth, Yukifumi NawaAbstract:Matrix metalloproteinase (MMPs) is the extracellular zinc-dependent endopeptidase and is secreted for degrading extracellular matrix molecules of host tissues. A cDNA encoding MMP-like protein of Gnathostoma spinigerum larvae was amplified by reverse transcription-polymerase chain reaction, and was cloned into a prokaryotic expression vector, and expressed in Escherichia coli. Total immunoglobulin G class (total IgG) antibody responses to the recombinant MMP-like protein were analyzed by immunoblot diagnosis of human Gnathostomiasis. Serum samples from proven and clinically suspected cases of Gnathostomiasis, other parasitic diseases patients, and from healthy volunteers were tested. The immunoblotting gave high sensitivity (100%) and specificity (94.7%). Positive and negative predictive values were 85.4% and 100%, respectively. Recombinant MMP-like protein can be used as a diagnostic antigen and potentially replace native parasite antigens to develop a Gnathostomiasis diagnostic kit.
Paron Dekumyoy - One of the best experts on this subject based on the ideXlab platform.
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MR Imaging Findings in Cerebrospinal
2015Co-Authors: Kittisak Sawanyawisuth, Jaturat Kanpittaya, Paron Dekumyoy, Somsak Tiamkao, Suthipun JitpimolmardAbstract:Summary: Human Gnathostomiasis is an infection caused mainly by Gnathostoma spinigerum, a nematode. Infected humans can present with various clinical manifestations. Serology is the criterion standard for diagnosing gnathos-tomiasis, whereas MR imaging represents a complemen-tary tool for assessing severity and extent of disease. We report two definite cases of Gnathostomiasis that were con-firmed by the immunoblotting technique. MR imaging of the cervical cords showed cord enlargement and diffuse high signal intensity, mainly of the gray-white matter re-gions. MR imaging of the brain showed hemorrhagic tract and scattered deep intracerebral hemorrhage with diffuse, fuzzy white matter lesions with nodular enhancement. Se-vere Gnathostomiasis was unresponsive to treatment. In northeast Thailand, consumption of raw fish, snake, or other intermediate hosts, such as chicken and duck, has made infection with Gnathostoma spini-gerum a common cause of asymmetrical paraparesis with eosinophilic CSF (1). Infection can also occur from contact with parasite-infested meats, unwashed utensils, and prenatal transmission. After exiting the intestine, the parasite may present as migratory swell-ing, ocular Gnathostomiasis, radiculitis, myelitis, sub-arachnoid hemorrhage, and/or intracerebral hemor-rhage. Clinical manifestations might involve one or several organs, with or without disease progression, depending on the route taken by the parasite. MR imaging of the cervical cord in Gnathostomiasis was first reported in France (2), in a case in which a patient presented with a cord lesion and MR imaging was helpful in diagnosing and assessing the severity of disease. Here we report the MR findings of two cases of clinically diagnosed Gnathostomiasis that were con-firmed by serology
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diagnosis of Gnathostomiasis by skin testing using partially purified specific antigen and total ige levels
Transactions of The Royal Society of Tropical Medicine and Hygiene, 2014Co-Authors: Wirach Maekanantawat, Supaporn Nuamtanong, Valai Bussaratid, Benjaluck Phonrat, Wallop Pakdee, Paron DekumyoyAbstract:BACKGROUND: A finding of antibodies to Gnathostoma spinigerum 24-kDa antigen by immunoblot analysis is currently used to confirm a diagnosis of Gnathostomiasis. A simple skin test for the diagnosis of Gnathostomiasis was developed, and the results were evaluated and compared with the standard Western blot (WB) test. METHODS: This cross-sectional study was conducted at the Hospital for Tropical Diseases, Bangkok, Thailand, in 2008-2011. All eligible patients were tested with partially purified proteins of mAb-detected fractions pooled and sterilized by 0.2 μm diameter syringe filter, with a phenol saline solution of 1:10 w/v. RESULTS: A total of 69 cases, 39 Gnathostomiasis cases and 30 controls, were enrolled into the study; the median age (IQR) was 40 (30.5-52.5) years. The most common presenting symptom was edema (56/69, 81%). Gnathostomiasis cases having strong cutaneous reactions to the intradermal test (81%) were also positive by immunoblot. A significant correlation between skin and immunoblot tests was detected (p<0.001). The difference in total IgE levels between cases and controls was not statistically significant (p=0.51). Logistic regression models showed that positive WB and skin-test results were significantly associated with Gnathostomiasis (p=0.001 and p=0.007, respectively). CONCLUSION: Gnathostoma skin testing, using prepared fractionated antigen solution of Gnathostoma spinigerum, yields good reactivity and significantly correlates with the results of immunoblot testing.
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Diagnosis of Gnathostomiasis by skin testing using partially purified specific antigen and total IgE levels.
Transactions of The Royal Society of Tropical Medicine and Hygiene, 2014Co-Authors: Wirach Maek-a-nantawat, Supaporn Nuamtanong, Valai Bussaratid, Benjaluck Phonrat, Wallop Pakdee, Paron DekumyoyAbstract:BACKGROUND: A finding of antibodies to Gnathostoma spinigerum 24-kDa antigen by immunoblot analysis is currently used to confirm a diagnosis of Gnathostomiasis. A simple skin test for the diagnosis of Gnathostomiasis was developed, and the results were evaluated and compared with the standard Western blot (WB) test. METHODS: This cross-sectional study was conducted at the Hospital for Tropical Diseases, Bangkok, Thailand, in 2008-2011. All eligible patients were tested with partially purified proteins of mAb-detected fractions pooled and sterilized by 0.2 μm diameter syringe filter, with a phenol saline solution of 1:10 w/v. RESULTS: A total of 69 cases, 39 Gnathostomiasis cases and 30 controls, were enrolled into the study; the median age (IQR) was 40 (30.5-52.5) years. The most common presenting symptom was edema (56/69, 81%). Gnathostomiasis cases having strong cutaneous reactions to the intradermal test (81%) were also positive by immunoblot. A significant correlation between skin and immunoblot tests was detected (p
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Diagnosis of Gnathostomiasis by skin testing using partially purified specific antigen and total IgE levels.
Transactions of the Royal Society of Tropical Medicine and Hygiene, 2014Co-Authors: Wirach Maek-a-nantawat, Supaporn Nuamtanong, Valai Bussaratid, Benjaluck Phonrat, Wallop Pakdee, Paron DekumyoyAbstract:BACKGROUND: A finding of antibodies to Gnathostoma spinigerum 24-kDa antigen by immunoblot analysis is currently used to confirm a diagnosis of Gnathostomiasis. A simple skin test for the diagnosis of Gnathostomiasis was developed, and the results were evaluated and compared with the standard Western blot (WB) test. METHODS: This cross-sectional study was conducted at the Hospital for Tropical Diseases, Bangkok, Thailand, in 2008-2011. All eligible patients were tested with partially purified proteins of mAb-detected fractions pooled and sterilized by 0.2 μm diameter syringe filter, with a phenol saline solution of 1:10 w/v. RESULTS: A total of 69 cases, 39 Gnathostomiasis cases and 30 controls, were enrolled into the study; the median age (IQR) was 40 (30.5-52.5) years. The most common presenting symptom was edema (56/69, 81%). Gnathostomiasis cases having strong cutaneous reactions to the intradermal test (81%) were also positive by immunoblot. A significant correlation between skin and immunoblot tests was detected (p
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Gnathostomiasis: An Emerging Imported Disease
2013Co-Authors: David A. J. Moore, Paron Dekumyoy, Janice Mccroddan, Peter L. ChiodiniAbstract:As the scope of international travel expands, an increasing number of travelers are coming into contact with helminthic parasites rarely seen outside the tropics. As a result, the occurrence of Gnathostoma spinigerum infection leading to the clinical syndrome Gnathostomiasis is increasing. In areas where Gnathostoma is not endemic, few clinicians are familiar with this disease. To highlight this underdiagnosed parasitic infection, we describe a case series of patients with Gnathostomiasis who were treated during a 12-month period at the Hospital for Tropica
Yukifumi Nawa - One of the best experts on this subject based on the ideXlab platform.
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Ocular Gnathostomiasis-Update of Earlier Survey.
The American journal of tropical medicine and hygiene, 2017Co-Authors: Yukifumi Nawa, Verajit Chotmongkol, Kittisak Sawanyawisuth, Masahide Yoshikawa, Sandra Fernandez Figueiras, Maria D Benavides, Sylvia Páz Díaz CamachoAbstract:Ocular Gnathostomiasis is rather a rare food-borne zoonosis caused by infection with the larvae of several species of genus Gnathostoma and is a representative ocular larva migrans syndrome. In our previous literature survey, we found 73 cases of ocular Gnathostomiasis reported up to and including 2009, though additional sporadic cases have been reported in Asia and the Americas since that report. Here, we review 10 additional cases reported since 2010, and also update current findings regarding epidemiological and clinical features in affected patients.
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cross reactivity pattern of asian and american human Gnathostomiasis in western blot assays using crude antigens prepared from gnathostoma spinigerum and gnathostoma binucleatum third stage larvae
American Journal of Tropical Medicine and Hygiene, 2016Co-Authors: Andreas Neumayr, Jose Ollague, Pham Ngoc Doanh, Eduardo Gotuzzo, Pedro Jimenez, Scott A. Norton, Francisco Bravo, Yukifumi Nawa, Yoichiro HoriiAbstract:Gnathostomiasis is a zoonotic parasitosis endemic in many Asian and some Latin American countries. Most human infections are caused by Gnathostoma spinigerum in Asia and Gnathostoma binucleatum in the Americas, and recently, imported cases have been increasing among travelers returning from endemic regions. Confirmation of the clinical diagnosis relies largely on serologic tests, with a G. spinigerum-antigen-based immunoblot currently being the diagnostic method of choice. However, we repeatedly experienced that sera from patients with clinically suspected American Gnathostomiasis gave negative results in this assay. Therefore, we used homologous methods to prepare G. spinigerum- and G. binucleatum-antigen-based immunoblot assays, and evaluated the cross-reactivity of the two assays. The results show incomplete cross-reactivity between the two assays: the G. spinigerum-antigen-based immunoblot apparently only detects Asian Gnathostomiasis caused by G. spinigerum, whereas the G. binucleatum-antigen-based immunoblot is apparently capable of detecting American as well as Asian Gnathostomiasis.
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AN OVERVIEW OF Gnathostomiasis IN THE WORLD
2015Co-Authors: Yukifumi Nawa, Fukumi Nakamura-uchiyamaAbstract:Abstract. Gnathostomiasis is a typical fish-borne parasitic zoonosis. Gnathostoma spinigerum has long been considered the only species to cause human infection. Because of the distribution of this species and the eating habits of the people, Gnathostomiasis was thought an unique parasitic disease in Asia, especially in Thailand and Japan. Around the 1970s, however, the presence of this disease was reported from Ecuador and Mexico, and now the endemicity in Latin America is considered the worst in the world. The causative species was recently proven to be G. binucleatum. In Japan, while Gnathostomiasis due to G. spinigerum had almost disappeared by the 1970s, small outbreaks and/or sporadic cases caused by infection with G. hispidum, G. doloresi, and G. nipponicum were, one after another, discovered during the 1980s-1990s, suggesting that any Gnathostoma spp would cause human disease. Apart from those known endemics, an outbreak of Gnathostomiasis among Korean emigrants in Yangon, Myanmar, was reported. Furthermore, collaborative study with Danish and Vietnamese parasitologists revealed that G. spinigerum infection is common in freshwater fishes in Vietnam, suggesting that Gnathostomiasis would occur all over the Indochina peninsula. In fact, this expectation will be substantiated by the country report from Vietnam in this meeting. In addition to those known or predicted endemic areas, literature survey revealed that Gnathostomiasis patients have been seen sporadically in Europe and North America. Those patients seen in non-endemic areas were mostly emigrants from endemic areas, but a few travelers from non-endemic areas als
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application of recombinant gnathostoma spinigerum matrix metalloproteinase like protein for serodiagnosis of human Gnathostomiasis by immunoblotting
American Journal of Tropical Medicine and Hygiene, 2013Co-Authors: Penchom Janwan, Porntip Laummaunwai, Chaisiri Wongkham, Pewpan M. Intapan, Viraphong Lulitanond, Chatchai Tayapiwatana, Hiroshi Yamasaki, Amnat Kitkhuandee, Kittisak Sawanyawisuth, Yukifumi NawaAbstract:Matrix metalloproteinase (MMPs) is the extracellular zinc-dependent endopeptidase and is secreted for degrading extracellular matrix molecules of host tissues. A cDNA encoding MMP-like protein of Gnathostoma spinigerum larvae was amplified by reverse transcription-polymerase chain reaction, and was cloned into a prokaryotic expression vector, and expressed in Escherichia coli. Total immunoglobulin G class (total IgG) antibody responses to the recombinant MMP-like protein were analyzed by immunoblot diagnosis of human Gnathostomiasis. Serum samples from proven and clinically suspected cases of Gnathostomiasis, other parasitic diseases patients, and from healthy volunteers were tested. The immunoblotting gave high sensitivity (100%) and specificity (94.7%). Positive and negative predictive values were 85.4% and 100%, respectively. Recombinant MMP-like protein can be used as a diagnostic antigen and potentially replace native parasite antigens to develop a Gnathostomiasis diagnostic kit.
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neuroGnathostomiasis a neglected parasitosis of the central nervous system
Emerging Infectious Diseases, 2011Co-Authors: Juri Katchanov, Verajit Chotmongkol, Kittisak Sawanyawisuth, Yukifumi NawaAbstract:Gnathostomiasis is a foodborne zoonotic helminthic infection caused by the third-stage larvae of Gnathostoma spp. nematodes. The most severe manifestation involves infection of the central nervous system, neuroGnathostomiasis. Although Gnathostomiasis is endemic to Asia and Latin America, almost all neuroGnathostomiasis cases are reported from Thailand. Despite high rates of illness and death, neuroGnathostomiasis has received less attention than the more common cutaneous form of Gnathostomiasis, possibly because of the apparent geographic confinement of the neurologic infection to 1 country. Recently, however, the disease has been reported in returned travelers in Europe. We reviewed the English-language literature on neuroGnathostomiasis and analyzed epidemiology and geographic distribution, mode of central nervous system invasion, pathophysiology, clinical features, neuroimaging data, and treatment options. On the basis of epidemiologic data, clinical signs, neuroimaging, and laboratory findings, we propose diagnostic criteria for neuroGnathostomiasis.
Verajit Chotmongkol - One of the best experts on this subject based on the ideXlab platform.
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Ocular Gnathostomiasis-Update of Earlier Survey.
The American journal of tropical medicine and hygiene, 2017Co-Authors: Yukifumi Nawa, Verajit Chotmongkol, Kittisak Sawanyawisuth, Masahide Yoshikawa, Sandra Fernandez Figueiras, Maria D Benavides, Sylvia Páz Díaz CamachoAbstract:Ocular Gnathostomiasis is rather a rare food-borne zoonosis caused by infection with the larvae of several species of genus Gnathostoma and is a representative ocular larva migrans syndrome. In our previous literature survey, we found 73 cases of ocular Gnathostomiasis reported up to and including 2009, though additional sporadic cases have been reported in Asia and the Americas since that report. Here, we review 10 additional cases reported since 2010, and also update current findings regarding epidemiological and clinical features in affected patients.
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ehmti 0025 clinical manifestations of subarachnoid hemorrhage from gnathostoma spinigerum in srinagarind hospital
Journal of Headache and Pain, 2014Co-Authors: Kittisak Sawanyawisuth, Jaturat Kanpittaya, Verajit Chotmongkol, Keetapong Pongtipakorn, Kannikar Kongbunkiat, Panita Limpawattana, Vichai Senthong, Jarin Chindaprasirt, W Waranon, Pewpan M. IntapanAbstract:Subarachnoid hemorrhage (SAH) is a serious neurological condition. Common cause of SAH is vascular origin. Gnathostomiasis is also a common disease in Thailand and may cause SAH.
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SUBARACHNOID HEMORRHAGE DUE TO VASCULAR CAUSES AND Gnathostomiasis: CLINICAL FEATURES AND LABORATORY FINDINGS
2014Co-Authors: Waranon Munkong, Jaturat Kanpittaya, Verajit Chotmongkol, Kittisak Sawanyawisuth, Keetapong Pongtipakorn, Kannikar Kongbunkiat, Panita Limpawattana, Vichai Senthong, Jarin Chindaprasirt, Pewpan M. IntapanAbstract:Subarachnoid hemorrhage (SAH) is a serious neurological condition, commonly of vascular etiology. Gnathostomiasis is a common parasitic disease in Thailand and may also cause SAH. The purpose of this study was to find clini - cal differences between SAH due to these two causes. This was a retrospective study and collected data from medical charts of patients diagnosed with SAH at Srinagarind Hospital, Khon Kaen, during 2009 and 2011. SAH due to vascular causes was diagnosed by cerebral angiogram, while cerebral Gnathostomiasis, in which cerebral angiograms were negative, was diagnosed immunologically. Differences in clinical features between the two groups were compared using de - scriptive statistics. Eighteen patients had SAH due to vascular causes and ten had Gnathostomiasis. Most parameters were similar between the two groups. However, the cerebrospinal fluid glucose /plasma glucose ratio in the Gnathostomiasis group was significantly higher than in the vascular group (80% vs 16.67%, respectively). In conclusion, cerebrospinal fluid glucose /plasma glucose ratio was significantly higher in SAH patients caused by Gnathostomiasis than vascular group and may provide a diagnostic tool for distinguishing between these two etiologies.
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A comparative study of neuroimaging features between human neuro-Gnathostomiasis and angiostrongyliasis
Neurological Sciences, 2012Co-Authors: Jaturat Kanpittaya, Pewpan M. Intapan, Verajit Chotmongkol, Kittisak Sawanyawisuth, Piyarat Khotsri, Wanchai MaleewongAbstract:Gnathostoma spinigerum and Angiostrongylus cantonensis are human parasites that can cause neurological symptoms. The human diseases produced by these parasites can usually be differentiated by clinical symptoms. The aim of this study was to report neuroimaging abnormalities detected with computed tomography (CT) and MR in patients with Gnathostomiasis and angiostrongyliasis. We enrolled 15 and 12 patients with serologically proven Gnathostomiasis and angiostrongyliasis, respectively, who had brain or spinal imaging done. The neuro-Gnathostomiasis group had significantly more patients with intracerebral hemorrhage and myelitis patterns. The angiostrongyliasis group had no specific findings and most patients had normal CT brain images. The variety of neuroimaging findings is shown here. This study emphasizes that neuroimaging studies may be useful to differentiate Gnathostomiasis and angiostrongyliasis particularly in patients with indistinct clinical presentations.
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specificity of immunoblotting analyses in eosinophilic meningitis
Memorias Do Instituto Oswaldo Cruz, 2011Co-Authors: Jaturat Kanpittaya, Pewpan M. Intapan, Verajit Chotmongkol, Kittisak Sawanyawisuth, Piyarat Khotsri, Wanchai MaleewongAbstract:Angiostrongylus cantonensis and Gnathostoma spinigerum are the two most common causative parasites of eosinophilic meningitis (EOM). Serological tests are helpful tools for confirming the identity of the pathogen. Recent reports determined the specificity of such tests by using normal healthy controls. There have been limited studies done to rule out the cross-reactivity between these two causative parasites of EOM. This study aims to assess the specificity of the serological test in EOM by using each condition as a control for the other. Thirty-three patients with a diagnosis of EOM were enrolled. Sera from 22 patients with a positive 29-kDa antigenic diagnostic band of A. cantonensis were tested for the 21 and 24-kDa antigenic bands of G. spinigerum. Similarly, sera of 11 Gnathostomiasis patients were tested for the 29-kDa diagnostic band for A. cantonensis. Only one patient in the angiostrongyliasis group had a positive result for the 21 and 24-kDa antigenic bands of G. spinigerum, while no Gnathostomiasis patients showed a positive result for the 29-kDa antigenic band of A. cantonensis. The specificity of the 21 and 24-kDa antigenic bands for Gnathostomiasis and the 29-kDa antigenic band for A. cantonensis was 95.5% and 100%, respectively. The antigenic bands for the diagnosis of Gnathostomiasis and angiostrongyliasis in EOM were highly specific.