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Tony Hadibarata - One of the best experts on this subject based on the ideXlab platform.
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Decolorization and degradation mechanism of Amaranth by Polyporus sp. S133
Bioprocess and Biosystems Engineering, 2014Co-Authors: Tony Hadibarata, Norazman Mohamad NorAbstract:Polyporus sp. S133 decolorized the Amaranth in 72 h (30 mg L(-1)) under static and shaking conditions. Liquid medium containing glucose has shown the highest decolorization of Amaranth by Polyporus sp. S133. When the effect of increasing inoculum concentration on decolorization of Amaranth was studied, maximum decolorization was observed with 15 % inoculum concentration. Significant increase in the enzyme production of laccase (102.2 U L(-1)) was observed over the period of Amaranth decolorization compared to lignin peroxidase and manganese peroxidase. Germination rate of Sorghum vulgare and Triticum aestivum was less with Amaranth treatment as compared to metabolites obtained after its decolorization. Based on the metabolites detected by GC-MS, it was proposed that Amaranth was bio-transformed into two intermediates, 1-hydroxy-2-naphthoic acid and 1,4-naphthaquinone. Overall findings suggested the ability of Polyporus sp. S133 for the decolorization of azo dye and ensured the ecofriendly degradation of Amaranth.
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Characterization of pyrene biodegradation by white‐rot fungus Polyporus sp. S133
Biotechnology and Applied Biochemistry, 2012Co-Authors: Tony Hadibarata, Risky Ayu Kristanti, Mohamad Ali Fulazzaky, Agung Endro NugrohoAbstract:A white-rot fungus of Polyporus sp. S133 was isolated from an oil-polluted soil. The metabolism of pyrene by this fungus was investigated in liquid medium with 5 mg of the compound. Depletion of pyrene was evident during the 30-day growth period and was 21% and 90%, respectively, in cometabolism and metabolism of pyrene alone. Pyrene was absorbed to fungal cells or biodegraded to form simpler structural compounds. Seventy-one percent of eliminated pyrene was transformed by Polyporus sp. S133 into other compounds, whereas only 18% was absorbed in the fungal cell. The effects of pH and temperature on biomass production of Polyporus sp. S133 for pyrene were examined; the properties of laccase and 1,2-dioxygenase produced by Polyporus sp. S133 during pyrene degradation were investigated. The optimal values of pH were 3, 5, and 4 for laccase, 1,2-dioxygenase, and biomass production, respectively, whereas the optimal values of temperature were 25 °C for laccase and 50 °C for 1,2-dioxygenase and biomass production. Under optimal conditions, pyrene was mainly metabolized to 1-hydroxypyrene and gentisic acid. The structure of 1-hydroxypyrene and gentisic acid was determined by gas chromatography-mass spectrometry after identification using thin-layer chromatography.
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identification of metabolites from benzo a pyrene oxidation by ligninolytic enzymes of Polyporus sp s133
Journal of Environmental Management, 2012Co-Authors: Tony Hadibarata, Risky Ayu KristantiAbstract:The biodegradation of benzo[a]pyrene (BaP) by using Polyporus sp. S133, a white-rot fungus isolated from oil-contaminated soil was investigated. Approximately 73% of the initial concentration of Ba Pw as degraded within 30 d of incubation. The isolation and characterization of 3 metabolites by thin layer chromatography, column chromatography, and UVevis spectrophotometry in combination with gas chromatographyemass spectrometry, indicated that Polyporus sp. S133 transformed Ba Pt o B aP1,6-quinone. This quinone was further degraded in 2 ways. First, BaP-1,6-quinone was decarboxylated and oxidized to form coumarin, which was then hydroxylated to hydroxycoumarin, and finally to hydroxyphenyl acetic acid by addition of an epoxide group. Second, Polyporus sp. S133 converted BaP-1,6-quinone into a major product, 1-hydroxy-2-naphthoic acid. During degradation, free extracellular laccase was detected with reduced activity of lignin peroxidase, manganese-dependent peroxidase and 2,3-dioxygenase, suggesting that laccase and 1,2-dioxygenase might play an important role in the transformation of PAHs compounds.
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Acceleration of Anthraquinone-Type Dye Removal by White-Rot Fungus Under Optimized Environmental Conditions
Water Air & Soil Pollution, 2012Co-Authors: Tony Hadibarata, Abdull Rahim Mohd Yusoff, Risky Ayu KristantiAbstract:The decolorization of the recalcitrant dye Remazol Brilliant Blue R (RBBR) by the culture filtrate of Polyporus sp. S133 and the effect of various environmental factors were investigated. Both biodegradation and biosorption were playing an important role in bioremoval mechanisms. The highest biosorption of RBBR in Polyporus sp. S133 was shown by all carbon sources such as sucrose, glucose, fructose, and starch. No biosorption was shown by the addition of aromatic compounds and metal ions; 97.1 % RBBR decolorization was achieved in 120-rpm culture for 96 h, as compared to 49.5 % decolorization in stationary culture. Increasing the shaking rotation of the culture to more than 120 rpm was proven to give a negative effect on decolorization. The highest production of laccase was shown at pH 4 and constantly decreases when the pH level increases. The addition of glucose, ammonium tartrate, Cu^2+, and protocatechuic acid was the suitable environmental condition for RBBR decolorization. There was a positive relationship between all environmental conditions and laccase production in the decolorization of RBBR.
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breakdown products in the metabolic pathway of anthracene degradation by a ligninolytic fungus Polyporus sp s133
Water Air and Soil Pollution, 2012Co-Authors: Tony Hadibarata, Ameer Badr Khudhair, Mohd Razman SalimAbstract:Polyporus sp. S133 fungi were selected based on their ability to degrade anthracene in liquid media. The degradation efficiency of anthracene increased by adding 0.5% Tween 80 to reach 71%; agitation at 120 rev/min increased the degradation to 92% after 30 days of incubation. Enzymes such as manganese peroxidase (MnP), lignin peroxidase (LiP), laccase, 1,2-dioxygenase and 2,3-dioxgenase were produced by Polyporus sp. S133 during incubation, and the highest enzyme activity was 182.3 U l−1 by 1,2-dioxygenase after 20 days of incubation. These results indicate that ligninolytic and dioxygenase enzymes secreted from Polyporus sp. S133 could play an important role in anthracene degradation efficiency. The metabolites detected through the degradation pathway were anthraquinone, phthalic acid, benzoic acid and catechol.
Risky Ayu Kristanti - One of the best experts on this subject based on the ideXlab platform.
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Characterization of pyrene biodegradation by white‐rot fungus Polyporus sp. S133
Biotechnology and Applied Biochemistry, 2012Co-Authors: Tony Hadibarata, Risky Ayu Kristanti, Mohamad Ali Fulazzaky, Agung Endro NugrohoAbstract:A white-rot fungus of Polyporus sp. S133 was isolated from an oil-polluted soil. The metabolism of pyrene by this fungus was investigated in liquid medium with 5 mg of the compound. Depletion of pyrene was evident during the 30-day growth period and was 21% and 90%, respectively, in cometabolism and metabolism of pyrene alone. Pyrene was absorbed to fungal cells or biodegraded to form simpler structural compounds. Seventy-one percent of eliminated pyrene was transformed by Polyporus sp. S133 into other compounds, whereas only 18% was absorbed in the fungal cell. The effects of pH and temperature on biomass production of Polyporus sp. S133 for pyrene were examined; the properties of laccase and 1,2-dioxygenase produced by Polyporus sp. S133 during pyrene degradation were investigated. The optimal values of pH were 3, 5, and 4 for laccase, 1,2-dioxygenase, and biomass production, respectively, whereas the optimal values of temperature were 25 °C for laccase and 50 °C for 1,2-dioxygenase and biomass production. Under optimal conditions, pyrene was mainly metabolized to 1-hydroxypyrene and gentisic acid. The structure of 1-hydroxypyrene and gentisic acid was determined by gas chromatography-mass spectrometry after identification using thin-layer chromatography.
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identification of metabolites from benzo a pyrene oxidation by ligninolytic enzymes of Polyporus sp s133
Journal of Environmental Management, 2012Co-Authors: Tony Hadibarata, Risky Ayu KristantiAbstract:The biodegradation of benzo[a]pyrene (BaP) by using Polyporus sp. S133, a white-rot fungus isolated from oil-contaminated soil was investigated. Approximately 73% of the initial concentration of Ba Pw as degraded within 30 d of incubation. The isolation and characterization of 3 metabolites by thin layer chromatography, column chromatography, and UVevis spectrophotometry in combination with gas chromatographyemass spectrometry, indicated that Polyporus sp. S133 transformed Ba Pt o B aP1,6-quinone. This quinone was further degraded in 2 ways. First, BaP-1,6-quinone was decarboxylated and oxidized to form coumarin, which was then hydroxylated to hydroxycoumarin, and finally to hydroxyphenyl acetic acid by addition of an epoxide group. Second, Polyporus sp. S133 converted BaP-1,6-quinone into a major product, 1-hydroxy-2-naphthoic acid. During degradation, free extracellular laccase was detected with reduced activity of lignin peroxidase, manganese-dependent peroxidase and 2,3-dioxygenase, suggesting that laccase and 1,2-dioxygenase might play an important role in the transformation of PAHs compounds.
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Acceleration of Anthraquinone-Type Dye Removal by White-Rot Fungus Under Optimized Environmental Conditions
Water Air & Soil Pollution, 2012Co-Authors: Tony Hadibarata, Abdull Rahim Mohd Yusoff, Risky Ayu KristantiAbstract:The decolorization of the recalcitrant dye Remazol Brilliant Blue R (RBBR) by the culture filtrate of Polyporus sp. S133 and the effect of various environmental factors were investigated. Both biodegradation and biosorption were playing an important role in bioremoval mechanisms. The highest biosorption of RBBR in Polyporus sp. S133 was shown by all carbon sources such as sucrose, glucose, fructose, and starch. No biosorption was shown by the addition of aromatic compounds and metal ions; 97.1 % RBBR decolorization was achieved in 120-rpm culture for 96 h, as compared to 49.5 % decolorization in stationary culture. Increasing the shaking rotation of the culture to more than 120 rpm was proven to give a negative effect on decolorization. The highest production of laccase was shown at pH 4 and constantly decreases when the pH level increases. The addition of glucose, ammonium tartrate, Cu^2+, and protocatechuic acid was the suitable environmental condition for RBBR decolorization. There was a positive relationship between all environmental conditions and laccase production in the decolorization of RBBR.
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decolorization and metabolism of anthraquionone type dye by laccase of white rot fungi Polyporus sp s133
Water Air and Soil Pollution, 2012Co-Authors: Tony Hadibarata, Abdull Rahim Mohd Yusoff, Risky Ayu KristantiAbstract:Utilization of microbes including white-rot fungi andbacterial strains for decolorizationofsynthetic dyes is one promising strategy of an environmentally friendly and cost-competitive alternative to physico- chemicaldecomposition processesfor treatingindustrial effluents. In this study, the biodegradation ability of the white-rot fungi Polyporus sp. S133 that produce high laccase was investigated in order to decolorize anthraquinone-type dye. Parameter including pH, tem- perature, and non-ionic surfactant were used to compar- atively study the decolorizing effects on Remazol Brilliant Blue R (RBBR). The purified laccase totally decolorized 200 mg L �1 initial concentration of RBBR dye when only 1.5 UL �1 of laccase was used in the reaction mixture. The optimal decolorization rates were achieved at pH 5 and at a temperature of 50°C. N-hydroxybenzotriazole, a small molecular weight redox mediator, was found to accelerate the decolorization. Tween 20 inhibited the decolorization while Tween 80 and Brij 35 showed no inhibition effect. Two compounds were identified as the intermediates (m/z 304.3 and m/z 342.2). These results suggest that laccase from Polyporus sp. S133 is a powerful tool for the decolorization of anthraquinone dyes. A pathway for the metabolism of the RBBR by laccase of Polyporus sp S133 was proposed. These proposed pathways could contribute to a better comprehension of the mechanisms used by oxidative enzymes to transform organic compounds.
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bioremediation of crude oil by white rot fungi Polyporus sp s133
Journal of Microbiology and Biotechnology, 2011Co-Authors: Risky Ayu Kristanti, Tony Hadibarata, Tadashi Toyama, Yasuhiro Tanaka, Kazuhiro MoriAbstract:The bioremediation potential of crude oil by Polyporus sp. S133 pre-grown in wood meal was investigated in two separate experiment trials; liquid medium and soil. The effect of three nutrients (glucose, polypeptone, and wood meal), oxygen flow, and some absorbent on the efficiency of the process was also evaluated. Degradation of crude oil in soil was significantly increased with an addition of oxygen flow and some absorbent (kapok and pulp). The highest degradation rate of crude oil was 93% in the soil with an addition of 10% kapok. The present study clearly demonstrates that, if suitably developed, Polyporus sp. S133 could be used to remediate soil contaminated with crude oil.
Sanro Tachibana - One of the best experts on this subject based on the ideXlab platform.
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identification of metabolites from phenanthrene oxidation by phenoloxidases and dioxygenases of Polyporus sp s133
Journal of Microbiology and Biotechnology, 2011Co-Authors: Tony Hadibarata, Sanro Tachibana, Muhamad AskariAbstract:Phenanthrene degradation by Polyporus sp. S133, a new phenanthrene-degrading strain, was investigated in this work. The analysis of degradation was performed by calculation of the remaining phenanthrene by gas chromatography - mass spectrometry. When cells were grown in phenanthrene culture after 92 h, all but 200 and 250 mg/l of the phenanthrene had been degraded. New metabolic pathways of phenanthrene and a better understanding of the phenoloxidases and dioxygenase mechanism involved in degradation of phenanthrene were explored in this research. The mechanism of degradation was determined through identification of the several metabolites; 9,10-phenanthrenequinone, 2,2'-diphenic acid, salicylic acid, and catechol. 9,10-Oxidation and ring cleavage to give 9,10-phenanthrenequinone is the major fate of phenanthrene in ligninolytic Polyporus sp. S133. The identification of 2,2'-diphenic acid in culture extracts indicates that phenanthrene was initially attacked through dioxigenation at C9 and C10 to give cis-9,10-dihydrodiol. Dehydrogenation of phenanthrene-cis-9,10-dihydrodiol to produce the corresponding diol, followed by ortho-cleavage of the oxygenated ring, produced 2,2'-diphenic acid. Several enzymes (manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase, and 2,3-dioxygenase) produced by Polyporus sp. S133 was detected during the incubation. The highest level of activity was shown at 92 h of culture.
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characterization of phenanthrene degradation by strain Polyporus sp s133
Journal of Environmental Sciences-china, 2010Co-Authors: Tony Hadibarata, Sanro TachibanaAbstract:Abstract Polyporus sp. S133, a fungus collected from contaminated soil, was used to degrade phenanthrene, a polycyclic aromatic hydrocarbon, in a mineral salt broth liquid culture. A maximal degradation rate (92%) was obtained when Polyporus sp. S133 was cultured for 30 days with agitation at 120 r/min, as compared to 44% degradation in non-agitated cultures. Furthermore, the degradation was affected by the addition of surfactants. Tween 80 was the most suitable surfactant for the degradation of phenanthrene by Polyporus sp. S133. The degradation rate increased as the amount of Tween 80 added increased. The rate in agitated cultures was about 2 times that in non-agitated cultures. The mechanism of degradation was determined through the identification of metabolites; 9,10-phenanthrenequinone, 2,2′-diphenic acid, phthalic acid, and protocatechuic acid. Several enzymes (manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase) produced by Polyporus sp. S133 were detected during the incubation. The highest level of activity was shown by 1,2-dioxygenase (187.4 U/L) after 20 days of culture.
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biodegradation of chrysene an aromatic hydrocarbon by Polyporus sp s133 in liquid medium
Journal of Hazardous Materials, 2009Co-Authors: Tony Hadibarata, Sanro Tachibana, Kazutaka ItohAbstract:Abstract Polyporus sp. S133, a fungus collected from contaminated-soil was used to degrade chrysene, a polycyclic aromatic hydrocarbon (PAH) in a mineral salt broth (MSB) liquid culture. Maximal degradation rate of chrysene (65%) was obtained when Polyporus sp. S133 was incubated in the cultures supplemented with polypeptone (10%) for 30 days under agitation of 120 rpm, as compared to just 24% degradation rate in non-agitated culture. Furthermore, the degradation of chrysene was affected by the addition of carbon and nitrogen sources as well as kind of surfactants. The degradation rate was increased with increase in added amount of carbon and nitrogen sources, respectively. The degradation rate in agitated cultures was enhanced about 2 times higher than that in non-agitated cultures. The degradation mechanism of chrysene by Polyporus sp. S133 was determined through identification of several metabolites; chrysenequinone, 1-hydroxy-2-naphthoic acid, phthalic acid, salicylic acid, protocatechuic acid, gentisic acid, and catechol. Several enzymes (manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase) produced by Polyporus sp. S133 were detected during the incubation. The highest enzyme activity was shown by 1,2-dioxygenase (237.5 U l−1) after 20 days of incubation.
Norazman Mohamad Nor - One of the best experts on this subject based on the ideXlab platform.
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Decolorization and degradation mechanism of Amaranth by Polyporus sp. S133
Bioprocess and Biosystems Engineering, 2014Co-Authors: Tony Hadibarata, Norazman Mohamad NorAbstract:Polyporus sp. S133 decolorized the Amaranth in 72 h (30 mg L(-1)) under static and shaking conditions. Liquid medium containing glucose has shown the highest decolorization of Amaranth by Polyporus sp. S133. When the effect of increasing inoculum concentration on decolorization of Amaranth was studied, maximum decolorization was observed with 15 % inoculum concentration. Significant increase in the enzyme production of laccase (102.2 U L(-1)) was observed over the period of Amaranth decolorization compared to lignin peroxidase and manganese peroxidase. Germination rate of Sorghum vulgare and Triticum aestivum was less with Amaranth treatment as compared to metabolites obtained after its decolorization. Based on the metabolites detected by GC-MS, it was proposed that Amaranth was bio-transformed into two intermediates, 1-hydroxy-2-naphthoic acid and 1,4-naphthaquinone. Overall findings suggested the ability of Polyporus sp. S133 for the decolorization of azo dye and ensured the ecofriendly degradation of Amaranth.
Yingyong Zhao - One of the best experts on this subject based on the ideXlab platform.
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traditional uses phytochemistry pharmacology pharmacokinetics and quality control of Polyporus umbellatus pers fries a review
Journal of Ethnopharmacology, 2013Co-Authors: Yingyong ZhaoAbstract:Abstract Ethnopharmacological relevance Polyporus umbellatus (Pers.) Fries (Polyporaceae, Zhuling ) has been commonly used in medicine for a wide range of ailments related to the edema, scanty urine, vaginal discharge, urinary dysfunction, as well as jaundice and diarrhea. Aim of the review The present paper reviewed the traditional uses, propagation, phytochemistry, pharmacology, pharmacokinetics and quality control of Polyporus umbellatus. Materials and methods All the available information on Polyporus umbellatus was collected via a library and electronic search (using Web of Science, Pubmed, ScienceDirect, Splinker, Google Scholar, etc.). Results Phytochemical studies showed the presence of many valuable secondary metabolites such as steroids, polysaccharides, anthraquinones and nucleosides. Crude extracts and isolated compounds showed a wide spectrum of pharmacological activities including diuretic, nephroprotective, anti-cancer, immuno-enhancing, hepatoprotective, anti-inflammatory and antioxidative activities. The pharmacokinetic studies demonstrated that the ergosterol and ergone had a high distribution and absorption in the plasma and the two main components of Polyporus umbellatus were mainly excreted by faeces. The determination of multiple chemical components was successfully applied to the quality control of Polyporus umbellatus. Conclusions Modern phytochemical, pharmacological and metabonomic investigations showed that the crude extracts and isolated compounds from Polyporus umbellatus possess many kinds of biological functions, especially in the diuretic activities and the treatment of kidney diseases as well as anti-cancer, immuno-enhancing and hepatoprotective activities. The pathways of the distribution, absorption, metabolism and excretion of main steroidal compounds were clarified by pharmacokinetic studies. Most of the pharmacological studies were conducted using crude and poorly characterized extracts of Polyporus umbellatus in animals especially in case of diuretic activities and the treatment of kidney diseases. Thus, more bioactive components especially diuretic compounds should be identified using bioactivity-guided isolation strategies and the possible mechanism of action as well as potential synergistic or antagonistic effects of multi-component mixtures derived from Polyporus umbellatus need to be evaluated integrating pharmacological, pharmacokinetic, bioavailability-centered and physiological approaches. In addition, more experiments including in vitro, in vivo and clinical studies should be encouraged to identify any side effects or toxicity. These achievements will further expand the existing therapeutic potential of Polyporus umbellatus and provide a beneficial support to its future further clinical use in modern medicine.
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bioactivity directed isolation identification of diuretic compounds from Polyporus umbellatus
Journal of Ethnopharmacology, 2009Co-Authors: Yingyong Zhao, Xu Chao, Yongmin ZhangAbstract:Abstract Aim of the study Polyporus umbellatus is a fungus used as a diuretic medicine. The objective of this study was to isolate and elucidate the diuretic constituents of n-hexane, ethyl acetate, n-butanol and water extracts of Polyporus umbellatus and to evaluate their diuretic activity. Materials and methods The n-hexane, ethyl acetate, n-butanol and water extracts of Polyporus umbellatus were tested by diuretic experiment of normal rats in metabolic cage. The n-hexane extract and n-butanol extract were prepared separately by the bioassay-guided approach. Three isolated compounds doses (5, 10 and 20 mg/kg BW) were orally administered to normal rats. Water excretion rate, pH and content of Na+, K+ and Cl− were measured in the urine of saline-loaded rats. Results n-Hexane extract (P d -mannitol) displayed diuretic activity. Conclusions The ergosta-4,6,8(14),22-tetraen-3-one was the strongest diuretic constituent in the three compounds. Ergosterol and d -mannitol were found to be also responsible for duiretic effects in Polyporus umbellatus for the first time. Data show that 20 mg/kg dose of the ergosterol for urine out put became significantly higher than in the control rats, but the ratio of Na+/K+ almost unaltered in the three doses. The highest dose of the d -mannitol was significant and increased the cumulative urine output. Regarding the electrolyte excretion, data show that the doses 10 and 20 mg/kg produce significant increase for excretion of Na+ and Cl−. The present results provide a quantitative basis explaining application of Polyporus umbellatus as a diuretic medicine. The result proved that its diuretic effects were also due to the contribution of multi-components in clinical application.
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simultaneous determination of eight major steroids from Polyporus umbellatus by high performance liquid chromatography coupled with mass spectrometry detections
Biomedical Chromatography, 2009Co-Authors: Yingyong Zhao, Yongmin Zhang, Xianlong Cheng, Ye ZhaoAbstract:Polyporus umbellatus is a widely used diuretic herbal medicine. In this study, a high-performance liquid chroma- tography coupled with atmospheric pressure chemical ionization-mass spectrometric detection (HPLC-APCI-MS) method was developed for qualitative and quantitative analysis of steroids, as well as for the quality control of Polyporus umbellatus. The selectivity, reproducibility and sensitivity were compared with HPLC with photodiode array detection and evaporative light scattering detection (ELSD). Selective ion monitoring in positive mode was used for qualitative and quantitative analysis of eight major components and b-ecdysterone was used as the internal standard. Limits of detection and quantifi cation fell in the ranges 7-21 and 18-63 ng/mL for the eight analytes with an injection of 10 mL samples, and all calibration curves showed good linear regression (r 2 > 0.9919) within the test range. The quantitative results demonstrated that samples from diff erent localities showed diff erent qualities. Advantages, in comparison with conventional HPLC-diode array detection and HPLC- ELSD, are that reliable identifi cation of target compounds could be achieved by accurate mass measurements along with characteristic retention time, and the great enhancement in selectivity and sensitivity allows identifi cation and quantifi cation of low levels of constituents in complex Polyporus umbellatus matrixes. Copyright © 2009 John Wiley & Sons, Ltd.
