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David H Pashley - One of the best experts on this subject based on the ideXlab platform.
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Dislocation resistance of ProRoot Endo Sealer, a calcium silicate‐based root canal Sealer, from radicular dentine
International Endodontic Journal, 2020Co-Authors: Bradford P. Huffman, R N Weller, Carolyn M Primus, James L Gutmann, Luigi Pinna, David H PashleyAbstract:Aim To examine the dislocation resistance of three root canal Sealers from radicular dentine with and without immersion in a simulated body fluid (SBF), using a modified push-out test design that produced simulated canal spaces of uniform dimensions under identical cleaning and shaping conditions. Methodology Sixty single-rooted caries-free human canine teeth were used. Standardized simulated canal spaces were created using 0.04 taper ProFile instruments along the coronal, middle and apical thirds of longitudinal tooth slabs. Following NaOCl/ethylenediamine tetra-acetic acid cleaning, the cavities were filled with ProRoot Endo Sealer, AH Plus Jet or Pulp Canal Sealer. After setting, half of the cavities were tested with a fibre-optic light-illuminated push-out testing device. The rest were immersed in SBF for 4 weeks before push-out evaluation. Failure modes were examined with stereomicroscopy and field emission (FE)-scanning electron microscopy. Results Location of the Sealer-filled cavities did not affect push-out strengths. ProRoot Endo Sealer exhibited higher push-out strengths than the other two Sealers particularly after SBF storage (P
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setting properties and cytotoxicity evaluation of a premixed bioceramic root canal Sealer
Journal of Endodontics, 2011Co-Authors: Bethany A Loushine, Robert J Loushine, Thomas E Bryan, Norman R Weller, Stephen W Looney, Brian M Gillen, David H PashleyAbstract:Abstract Introduction This study investigated the setting time and micohardness of a premixed calcium phosphate silicate–based Sealer (EndoSequence BC Sealer; Brasseler USA, Savannah, GA) in the presence of different moisture contents (0–9 wt%). The moisture content that produced the most optimal setting properties was used to prepare set EndoSequence BC Sealer for cytotoxicity comparison with an epoxy resin–based Sealer (AH Plus; Dentsply Caulk, Milford, DE). Methods Standardized disks were created with BC Sealer, AH Plus, Pulp Canal Sealer EWT (positive control) (SybronEndo, Orange CA), and Teflon (Small Parts Inc., Miami Lakes, FL; negative control). Disks were placed in Transwell Inserts, providing indirect contact with MC3T3-E1 cells. Succinate dehydrogenase activity of the cells was evaluated over a 6-week period using MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Cytotoxicity profiles of BC Sealer and AH Plus were fitted with polynomial regression models. The time for 50% of the cells to survive ( T 0.5 ) was analyzed using the Wald statistic with a two-tailed significance level of 0.05. Results BC Sealer required at least 168 hours to reach the final setting using the Gilmore needle method, and its microhardeness significantly declined when water was included in the Sealer ( P = .004). All set Sealers exhibited severe cytotoxicity at 24 hours. The cytotoxicity of AH Plus gradually decreased and became noncytotoxic, whereas BC Sealer remained moderately cytotoxic over the 6-week period. A significant difference ( P T 0.5 of BC Sealer (5.10 weeks; 95% confidence interval [CI], 4.69–5.42, standard error [SE] = 0.09) and T 0.5 of AH Plus (0.86 weeks; 95% CI, 0.68–1.05; SE=0.18). Conclusions Further studies are required to evaluate the correlation between the length of setting time of BC Sealer and its degree of cytotoxicity.
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the efficacy of different Sealer removal protocols on bonding of self etching adhesives to ah plus contaminated dentin
Journal of Endodontics, 2009Co-Authors: Steven Roberts, Li Sha Gu, Quinn M Mitchell, David H PashleyAbstract:Abstract Introduction Smearing of unset root canal Sealers over the pulp chamber dentin may adversely affect bonding of self-etching adhesives and jeopardize their coronal sealing potential. This study examined the influence of different Sealer removal protocols on the microtensile bond strengths of two self-etching adhesives to AH Plus-contaminated dentin. Methods Coronal dentin surfaces were prepared from extracted human third molars. In the positive control groups, these surfaces were not contaminated with Sealer and were bonded with Clearfil SE Bond or Clearfil Tri-S Bond. For the experimental groups, dentin surfaces were contaminated with AH Plus and wiped with either dry cotton pellets, cotton pellets saturated with ethanol, or cotton pellets saturated with Endosolv R followed by rinsing the dissolved Sealer with water prior to bonding with the two adhesives. Bonded specimens were sectioned into resin-dentin beams for microtensile bond strength evaluation. Additional specimens were prepared for transmission electron microscopy to examine the ultrastructure and nanoleakage within the hybrid layers. Results For both adhesives, microtensile bond strengths significantly declined when the Sealer was removed with dry cotton pellets or cotton pellets saturated with ethanol. Only the Endosolv R/water Sealer removal protocol restored tensile bond strengths to those of the uncontaminated positive controls without adversely affecting hybrid layer formation in intact dentin or increasing nanoleakage within the resin-dentin interfaces. Conclusion The Endosolv R Sealer removal protocol appears to be effective in preventing the deterioration of bond strengths of the two self-etching adhesives to AH Plus–contaminated dentin and warrants further clinical investigation.
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dislocation resistance of proroot endo Sealer a calcium silicate based root canal Sealer from radicular dentine
International Endodontic Journal, 2009Co-Authors: Bradford P. Huffman, R N Weller, Carolyn M Primus, James L Gutmann, Luigi Pinna, David H PashleyAbstract:Aim To examine the dislocation resistance of three root canal Sealers from radicular dentine with and without immersion in a simulated body fluid (SBF), using a modified push-out test design that produced simulated canal spaces of uniform dimensions under identical cleaning and shaping conditions. Methodology Sixty single-rooted caries-free human canine teeth were used. Standardized simulated canal spaces were created using 0.04 taper ProFile instruments along the coronal, middle and apical thirds of longitudinal tooth slabs. Following NaOCl/ethylenediamine tetra-acetic acid cleaning, the cavities were filled with ProRoot Endo Sealer, AH Plus Jet or Pulp Canal Sealer. After setting, half of the cavities were tested with a fibre-optic light-illuminated push-out testing device. The rest were immersed in SBF for 4 weeks before push-out evaluation. Failure modes were examined with stereomicroscopy and field emission (FE)-scanning electron microscopy. Results Location of the Sealer-filled cavities did not affect push-out strengths. ProRoot Endo Sealer exhibited higher push-out strengths than the other two Sealers particularly after SBF storage (P < 0.001). Failure modes were predominantly adhesive and mixed for Pulp Canal Sealer and AH Plus Jet, and predominantly cohesive for ProRoot Endo Sealer. Spherical amorphous calcium phosphate-like phases that spontaneously transformed into apatite-like phases were seen in the fractured specimens of ProRoot Endo Sealer after SBF storage. Conclusions When tested in bulk without a main core, both ‘Sealer type’ and ‘SBF storage’ were significant in affecting push-out results. The ProRoot Endo Sealer demonstrated the presence of spherical amorphous calcium phosphate-like phases and apatite-like phases (i.e. ex vivo bioactivity) after SBF storage.
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microscopic appearance and apical seal of root canals filled with gutta percha and proroot endo Sealer after immersion in a phosphate containing fluid
International Endodontic Journal, 2008Co-Authors: R N Weller, L V Garrett, Carolyn M Primus, James L Gutmann, David H PashleyAbstract:Aim To investigate the sealing quality of ProRoot Endo Sealer, a calcium silicate-based Sealer and its morphologic characteristics after immersion in a phosphate-containing fluid (PCF). Methodology Single-rooted canals were filled with gutta-percha and either ProRoot Endo Sealer or two commercially available zinc oxide eugenol (ZOE)-based and epoxy resin-based Sealers. The Sealers were allowed to set for 6 days and the filled teeth were immersed in PCF for 24 h before fluid leakage evaluation. After initial leakage evaluation at the 7th day, each filled root was restored and reimmersed in PCF for 28 days before the second phase of leakage evaluation at 35 days. Cryofractured specimens of additional teeth filled with the three Sealers were examined using scanning electron microscopy after immersion in PCF for the two periods. Results One-way repeated measures anova and Tukey test revealed significant differences between the ZOE-based Sealer at 35 days and the calcium silicate-based Sealer at 35 days (P < 0.001), and between the ZOE-based Sealer at 7 days and the calcium silicate-based Sealer at 35 days (P = 0.001). No difference was found between the epoxy resin-based Sealer and the calcium silicate-based Sealer after both storage periods. Cryofractured calcium silicate-based Sealer specimens demonstrated apatite-like crystalline deposits along the apical and middle thirds of the canal walls via transformation from amorphous calcium phosphate-like precursors. Conclusions ProRoot Endo Sealer is comparable in sealing quality to the epoxy resin-based Sealer and seals better than the ZOE-based Sealer after immersion in PCF. The calcium silicate-based Sealer also demonstrates ex vivo bioactivity when it comes into contact with phosphate ions.
Josette Camilleri - One of the best experts on this subject based on the ideXlab platform.
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Interfacial Characteristics and Cytocompatibility of Hydraulic Sealer Cements.
Journal of Endodontics, 2018Co-Authors: Mira Kebudi Benezra, Pierre Schembri Wismayer, Josette CamilleriAbstract:Abstract Introduction The stability and long-term success of root canal obturation depends on the choice of Sealer because the Sealer bonds to the dentin and stabilizes the solid cone. Furthermore, the Sealer needs to be nontoxic because Sealer toxicity will certainly lead to treatment failure. The aim of this study was to assess the Sealer-dentin interface of 3 hydraulic root canal Sealers and to evaluate their cytocompatibility compared with AH Plus (Dentsply DeTrey GmbH, Konstanz, Germany). Methods Four dental root canal Sealers were assessed. AH Plus, MTA Fillapex (Angelus, Londrina, Brazil), BioRoot RCS (Septodont, Saint-Maur-des-Fosses, France), and Endoseal (Maruchi, Wonju-si, Gangwon-do, South Korea) were characterized using scanning electron microscopy and energy-dispersive spectroscopy. The Sealer-tooth interface was assessed by confocal microscopy and scanning electron microscopy, and biocompatibility was measured by assessing the cell metabolic function using direct contact assays and alkaline phosphatase activity. Results The tricalcium silicate–based Sealers presented a different microstructure and elemental composition despite their similar chemistry and classification. BioRoot RCS was free of aluminum, and all Sealers presented different radiopacifying elements. The Sealer penetration in the dentinal tubules and interfacial characteristics were different. The migration of silicon was evident from Sealer to tooth for all Sealers containing tricalcium silicate. MTA Fillapex and BioRoot RCS exhibited the best cytocompatibility in both the direct contact test and alkaline phosphatase activity. Conclusions The use of hydraulic calcium silicate–based Sealers has introduced a different material type to endodontics. These materials are different than other Sealers mostly because of their hydraulic nature and their interaction with the environment. Although the Sealers tested had a similar chemistry, their cytocompatibility and bonding mechanisms were diverse.
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Influence of environment on testing of hydraulic Sealers.
Scientific Reports, 2017Co-Authors: Mira Kebudi Benezra, Pierre Schembri Wismayer, Josette CamilleriAbstract:In vitro material testing is undertaken by conducting a series of tests following procedures outlined in international standards. All material properties are measured in water; however biological behavior is undertaken in alternative media such as Dulbecco’s modified eagle medium (DMEM) or simulated body fluid. The aim of this study was to characterize four dental root canal Sealers and study their properties in different media. Four dental root canal Sealers were assessed. They were characterized by a combination of techniques and the Sealer properties were tested as specified by ISO 6876 (2012) and also in alternative media. The Sealer biocompatibility was measured by cell function and proliferation assays of elutions. All Sealers complied with ISO specifications. The material properties were effected by the type of soaking medium used and the surface micromorphology and elemental composition were dependent on the soaking solution type. Both BioRoot and MTA Fillapex showed cytotoxicity which reduced at higher dilutions. The material chemistry, presentation, environmental conditions and testing methodology used affected the Sealer properties. Standards specific to Sealer type are thus indicated. Furthermore the methodology used in the standard testing should be more relevant to clinical situations.
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Properties of Tricalcium Silicate Sealers
Journal of Endodontics, 2016Co-Authors: Issam Khalil, Alfred Naaman, Josette CamilleriAbstract:Abstract Introduction Sealers based on tricalcium silicate cement aim at an interaction of the Sealer with the root canal wall, alkalinity with potential antimicrobial activity, and the ability to set in a wet field. The aim of this study was to characterize and investigate the properties of a new tricalcium silicate–based Sealer and verify its compliance to ISO 6876 (2012). Methods A new tricalcium silicate–based Sealer (Bio MM; St Joseph University, Beirut, Lebanon), BioRoot RCS (Septodont, St Maure de Fosses, France), and AH Plus (Dentsply, DeTrey, Konstanz, Germany) were investigated. Characterization using scanning electron microscopy, energy-dispersive spectroscopy, and X-ray diffraction analysis was performed. Furthermore, Sealer setting time, flow, film thickness, and radiopacity were performed following ISO specifications. pH and ion leaching in solution were assessed by pH analysis and inductively coupled plasma. Results Bio MM and BioRoot RCS were both composed of tricalcium silicate and tantalum oxide in Bio MM and zirconium oxide in BioRoot RCS. In addition, the Bio MM contained calcium carbonate and a phosphate phase. The inorganic components of AH Plus were calcium tungstate and zirconium oxide. AH Plus complied with the ISO norms for both flow and film thickness. BioRoot RCS and Bio MM exhibited a lower flow and a higher film thickness than that specified for Sealer cements in ISO 6876. All test Sealers exhibited adequate radiopacity. Conclusions Bio MM interacted with physiologic solution, thus showing potential for bioactivity. Sealer properties were acceptable and comparable with other Sealers available clinically.
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Calcium silicate-based Sealers: Assessment of physicochemical properties, porosity and hydration
Dental Materials, 2016Co-Authors: Marina Angélica Marciano, Marco Antonio Hungaro Duarte, Josette CamilleriAbstract:Objectives Investigation of hydration, chemical, physical properties and porosity of experimental calcium silicate-based Sealers. Methods Experimental calcium silicate-based Sealers with calcium tungstate and zirconium oxide radio-opacifiers were prepared by mixing 1 g of powder to 0.3 mL of 80% distilled water and 20% propylene glycol. MTA and MTA Fillapex were used as controls. The raw materials and set Sealers were characterized using a combination of scanning electron microscopy, energy dispersive spectroscopy and X-ray diffraction. Physical properties were analyzed according to ANSI/ADA. The pH and calcium ion release were assessed after 3, 24, 72 and 168 h. The porosity was assessed using mercury intrusion porosimetry. Results The analysis of hydration of prototype Sealers revealed calcium hydroxide as a by-product resulting in alkaline pH and detection of calcium ion release, with high values in initial periods. The radiopacity was similar to MTA for the Sealers containing high amounts of radio-opacifiers (p > 0.05). Flowability was higher and film thickness was lower for resinous MTA Fillapex Sealer (p < 0.05). The test Sealers showed water sorption and porosity similar to MTA (p > 0.05). Significance The prototype Sealers presented adequate hydration, elevated pH and calcium ion release. Regarding physical properties, elevated proportions of radio-opacifiers were necessary to accomplish adequate radiopacity, enhance flowability and reduce film thickness. All the tested Sealers presented water sorption and porosity similar to MTA.
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Interface of dentine to root canal Sealers.
Journal of Dentistry, 2013Co-Authors: Raqueli Viapiana, J. M. Guerreiro-tanomaru, Mário Tanomaru-filho, Josette CamilleriAbstract:Abstract Objective Root canal Sealers can interact physically or chemically with dentine. The aim of this study was to characterize the dentine-root canal Sealer interface of experimental Sealers based on Portland cement using an epoxy-based vehicle in comparison to an epoxy resin Sealer, AH Plus. Methods Root canals were biomechanically prepared and filled with any one of the four experimental epoxy Sealers containing Portland cement with micro- and nano-particles of either zirconium oxide or niobium oxide radiopacifers, or AH Plus. The dentine-Sealer's interfaces were assessed by coronal penetration of fluorescent microspheres, the penetration of Sealers labelled with Rhodamine B inside the dentine tubules (following obturation with gutta–percha and Sealers using System B technique) assessed by confocal laser scanning microscopy, and the chemical characterization of dentine-Sealers interface by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) line scans. Results No penetration of fluorescent microspheres at the root-dentine to Sealer interface was recorded for all test materials. Sealers presented greater ability to penetrate within the dentinal tubules at the coronal and mid-root thirds. The experimental Sealers containing radiopacifier nano-particles exhibited a more homogeneous microstructure along the whole length of the canal. EDS-line scans results showed a migration of silicon and niobium into dentine. Peak overlap between zirconium and the phosphorous compromised the identification of the migration of the zirconium oxide into dentine. Conclusions All five Sealers promoted coronal sealing. The experimental Sealers exhibited promising characteristics and were comparable to AH Plus Sealer. Elemental migration of the experimental Sealers suggests material interaction with dentine which was not displayed by AH Plus.
Carlo Prati - One of the best experts on this subject based on the ideXlab platform.
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properties of bioroot rcs a tricalcium silicate endodontic Sealer modified with povidone and polycarboxylate
International Endodontic Journal, 2017Co-Authors: Francesco Siboni, Carlo Prati, Paola Taddei, Fausto Zamparini, Maria Giovanna GandolfiAbstract:Aim To evaluate the chemical and physical properties of a tricalcium silicate root canal Sealer containing povidone and polycarboxylate (BioRoot RCS), a calcium silicate MTA based Sealer containing a salicylate resin (MTA Fillapex), a traditional eugenol-containing Sealer (Pulp Canal Sealer) and an epoxy resin-based root canal Sealer (AH Plus). Methodology Calcium release, pH, setting time, water sorption, (volume of open pores, volume of impervious portion, apparent porosity) porosity and weight loss were measured. The ability to nucleate calcium phosphates (CaP) after ageing 28 days in a simulated body fluid was evaluated using ESEM-EDX and micro-Raman spectroscopy. Data were statistically analyzed (p=0.05) using one-way ANOVA (setting time, radiopacity, solubility, water sorption, porosity) or two-way ANOVA (ion release tests). Results BioRoot RCS had a final setting time of 300 min and adequate radiopacity (5.2 mmAl). It demonstrated the highest (p<0.05) and more prolonged ability to release calcium ions (721 ppm at 3h) and to increase the pH (11-12) (p<0.05); B-type carbonated apatite deposits were found on aged BioRoot RCS (biointeractivity-related CaP-forming ability). A final setting time of 270 min and good calcium release (17.4 ppm at 3h) were measured for MTA Fillapex; apatite deposits were present on aged samples. No calcium release and no alkalizing activity were measured for Pulp Canal Sealer and AH Plus; no CaP nucleation was detected on aged Pulp Canal Sealer, and some apatite deposits were found on aged AH Plus (chemi/physisorption-related CaP-deposition). Higher and significantly different (p<0.05) porosity, water sorption and solubility were measured for the two calcium silicate Sealers, especially for BioRoot RCS. Conclusions BioRoot RCS had bioactivity with calcium release, strong alkalizing activity and apatite forming ability, adequate radiopacity. This article is protected by copyright. All rights reserved.
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properties of a novel polysiloxane guttapercha calcium silicate bioglass containing root canal Sealer
Dental Materials, 2016Co-Authors: Maria Giovanna Gandolfi, Francesco Siboni, Carlo PratiAbstract:Abstract Objective Root canal filling Sealers based on polymethyl hydrogensiloxane or polymethyl hydrogensiloxane-guttapercha – introduced to improve the quality of conventional guttapercha-based and resin-based systems – showed advantages in handiness and clinical application. The aim of the study was to evaluate the chemical–physical properties of a novel polysiloxane-guttapercha calcium silicate-containing root canal Sealer (GuttaFlow bioseal). Methods GuttaFlow bioseal was examined and compared with GuttaFlow2, RoekoSeal and MTA Fillapex Sealers. Setting times, open and impervious porosity and apparent porosity, water sorption, weight loss, calcium release, and alkalinizing activity were evaluated. ESEM-EDX-Raman analyses of fresh materials and after soaking in simulated body fluid were also performed. Results Marked differences were obtained among the materials. GuttaFlow bioseal showed low solubility and porosity, high water sorption, moderate calcium release and good alkalinizing activity. MTA Fillapex showed the highest calcium release, alkalinizing activity and solubility, RoekoSeal the lowest calcium release, no alkalinizing activity, very low solubility and water sorption. Only GuttaFlow bioseal showed apatite forming ability. Significance GuttaFlow bioseal showed alkalinizing activity together with negligible solubility and slight calcium release. Therefore, the notable nucleation of apatite and apatite precursors can be related to the co-operation of CaSi particles (Si OH groups) with polysiloxane (Si O Si groups). The incorporation of a calcium silicate component into polydimethyl polymethylhydrogensiloxane guttapercha Sealers may represent an attractive strategy to obtain a bioactive biointeractive flowable guttapercha Sealer for moist/bleeding apices with bone defects in endodontic therapy.
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dynamic sealing ability of mta root canal Sealer
International Endodontic Journal, 2011Co-Authors: Josette Camilleri, Maria Giovanna Gandolfi, Francesco Siboni, Carlo PratiAbstract:Camilleri J, Gandolfi MG, Siboni F, Prati C. Dynamic sealing ability of MTA root canal Sealer. International Endodontic Journal, 44, 9–20, 2011. Abstract Aims To evaluate (i) the sealing ability of two Sealers, mineral trioxide aggregate Sealer (MTAS) and Pulp Canal Sealer (PCS), used with gutta-percha utilizing the fluid filtration method, (ii) leaching and surface characteristics in Hank’s balanced salt solution (HBSS) over a period of time. Methodology Surface characteristics in HBSS were evaluated under the scanning electron microscope after 1 and 28 days, and the leaching of both Sealers were assessed by inductively coupled plasma atomic absorption spectrometry (ICP-AAS). In addition, 24 single rooted extracted teeth were root filled using warm vertical compaction with either MTAS or PCS used as Sealers with gutta-percha. Four teeth were used as positive and negative controls. Sealing ability was evaluated after 1 or 28 days using the fluid filtration method. Results Mineral trioxide aggregate Sealer exhibited crystalline deposits rich in calcium and phosphorus on its surface when in contact with a physiological solution. These crystalline deposits were absent in PCS and on MTAS stored at 100% humidity. The sealing ability of MTAS was similar to that of PCS. Conclusions The novel Sealer based on mineral trioxide aggregate had comparable sealing ability to a proprietary brand Sealer cement. In contact with a simulated body fluid, the MTA Sealer released calcium ions in solution that encouraged the deposition of calcium phosphate crystals.
R N Weller - One of the best experts on this subject based on the ideXlab platform.
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Dislocation resistance of ProRoot Endo Sealer, a calcium silicate‐based root canal Sealer, from radicular dentine
International Endodontic Journal, 2020Co-Authors: Bradford P. Huffman, R N Weller, Carolyn M Primus, James L Gutmann, Luigi Pinna, David H PashleyAbstract:Aim To examine the dislocation resistance of three root canal Sealers from radicular dentine with and without immersion in a simulated body fluid (SBF), using a modified push-out test design that produced simulated canal spaces of uniform dimensions under identical cleaning and shaping conditions. Methodology Sixty single-rooted caries-free human canine teeth were used. Standardized simulated canal spaces were created using 0.04 taper ProFile instruments along the coronal, middle and apical thirds of longitudinal tooth slabs. Following NaOCl/ethylenediamine tetra-acetic acid cleaning, the cavities were filled with ProRoot Endo Sealer, AH Plus Jet or Pulp Canal Sealer. After setting, half of the cavities were tested with a fibre-optic light-illuminated push-out testing device. The rest were immersed in SBF for 4 weeks before push-out evaluation. Failure modes were examined with stereomicroscopy and field emission (FE)-scanning electron microscopy. Results Location of the Sealer-filled cavities did not affect push-out strengths. ProRoot Endo Sealer exhibited higher push-out strengths than the other two Sealers particularly after SBF storage (P
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dislocation resistance of proroot endo Sealer a calcium silicate based root canal Sealer from radicular dentine
International Endodontic Journal, 2009Co-Authors: Bradford P. Huffman, R N Weller, Carolyn M Primus, James L Gutmann, Luigi Pinna, David H PashleyAbstract:Aim To examine the dislocation resistance of three root canal Sealers from radicular dentine with and without immersion in a simulated body fluid (SBF), using a modified push-out test design that produced simulated canal spaces of uniform dimensions under identical cleaning and shaping conditions. Methodology Sixty single-rooted caries-free human canine teeth were used. Standardized simulated canal spaces were created using 0.04 taper ProFile instruments along the coronal, middle and apical thirds of longitudinal tooth slabs. Following NaOCl/ethylenediamine tetra-acetic acid cleaning, the cavities were filled with ProRoot Endo Sealer, AH Plus Jet or Pulp Canal Sealer. After setting, half of the cavities were tested with a fibre-optic light-illuminated push-out testing device. The rest were immersed in SBF for 4 weeks before push-out evaluation. Failure modes were examined with stereomicroscopy and field emission (FE)-scanning electron microscopy. Results Location of the Sealer-filled cavities did not affect push-out strengths. ProRoot Endo Sealer exhibited higher push-out strengths than the other two Sealers particularly after SBF storage (P < 0.001). Failure modes were predominantly adhesive and mixed for Pulp Canal Sealer and AH Plus Jet, and predominantly cohesive for ProRoot Endo Sealer. Spherical amorphous calcium phosphate-like phases that spontaneously transformed into apatite-like phases were seen in the fractured specimens of ProRoot Endo Sealer after SBF storage. Conclusions When tested in bulk without a main core, both ‘Sealer type’ and ‘SBF storage’ were significant in affecting push-out results. The ProRoot Endo Sealer demonstrated the presence of spherical amorphous calcium phosphate-like phases and apatite-like phases (i.e. ex vivo bioactivity) after SBF storage.
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microscopic appearance and apical seal of root canals filled with gutta percha and proroot endo Sealer after immersion in a phosphate containing fluid
International Endodontic Journal, 2008Co-Authors: R N Weller, L V Garrett, Carolyn M Primus, James L Gutmann, David H PashleyAbstract:Aim To investigate the sealing quality of ProRoot Endo Sealer, a calcium silicate-based Sealer and its morphologic characteristics after immersion in a phosphate-containing fluid (PCF). Methodology Single-rooted canals were filled with gutta-percha and either ProRoot Endo Sealer or two commercially available zinc oxide eugenol (ZOE)-based and epoxy resin-based Sealers. The Sealers were allowed to set for 6 days and the filled teeth were immersed in PCF for 24 h before fluid leakage evaluation. After initial leakage evaluation at the 7th day, each filled root was restored and reimmersed in PCF for 28 days before the second phase of leakage evaluation at 35 days. Cryofractured specimens of additional teeth filled with the three Sealers were examined using scanning electron microscopy after immersion in PCF for the two periods. Results One-way repeated measures anova and Tukey test revealed significant differences between the ZOE-based Sealer at 35 days and the calcium silicate-based Sealer at 35 days (P < 0.001), and between the ZOE-based Sealer at 7 days and the calcium silicate-based Sealer at 35 days (P = 0.001). No difference was found between the epoxy resin-based Sealer and the calcium silicate-based Sealer after both storage periods. Cryofractured calcium silicate-based Sealer specimens demonstrated apatite-like crystalline deposits along the apical and middle thirds of the canal walls via transformation from amorphous calcium phosphate-like precursors. Conclusions ProRoot Endo Sealer is comparable in sealing quality to the epoxy resin-based Sealer and seals better than the ZOE-based Sealer after immersion in PCF. The calcium silicate-based Sealer also demonstrates ex vivo bioactivity when it comes into contact with phosphate ions.
Bradford P. Huffman - One of the best experts on this subject based on the ideXlab platform.
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Dislocation resistance of ProRoot Endo Sealer, a calcium silicate‐based root canal Sealer, from radicular dentine
International Endodontic Journal, 2020Co-Authors: Bradford P. Huffman, R N Weller, Carolyn M Primus, James L Gutmann, Luigi Pinna, David H PashleyAbstract:Aim To examine the dislocation resistance of three root canal Sealers from radicular dentine with and without immersion in a simulated body fluid (SBF), using a modified push-out test design that produced simulated canal spaces of uniform dimensions under identical cleaning and shaping conditions. Methodology Sixty single-rooted caries-free human canine teeth were used. Standardized simulated canal spaces were created using 0.04 taper ProFile instruments along the coronal, middle and apical thirds of longitudinal tooth slabs. Following NaOCl/ethylenediamine tetra-acetic acid cleaning, the cavities were filled with ProRoot Endo Sealer, AH Plus Jet or Pulp Canal Sealer. After setting, half of the cavities were tested with a fibre-optic light-illuminated push-out testing device. The rest were immersed in SBF for 4 weeks before push-out evaluation. Failure modes were examined with stereomicroscopy and field emission (FE)-scanning electron microscopy. Results Location of the Sealer-filled cavities did not affect push-out strengths. ProRoot Endo Sealer exhibited higher push-out strengths than the other two Sealers particularly after SBF storage (P
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dislocation resistance of proroot endo Sealer a calcium silicate based root canal Sealer from radicular dentine
International Endodontic Journal, 2009Co-Authors: Bradford P. Huffman, R N Weller, Carolyn M Primus, James L Gutmann, Luigi Pinna, David H PashleyAbstract:Aim To examine the dislocation resistance of three root canal Sealers from radicular dentine with and without immersion in a simulated body fluid (SBF), using a modified push-out test design that produced simulated canal spaces of uniform dimensions under identical cleaning and shaping conditions. Methodology Sixty single-rooted caries-free human canine teeth were used. Standardized simulated canal spaces were created using 0.04 taper ProFile instruments along the coronal, middle and apical thirds of longitudinal tooth slabs. Following NaOCl/ethylenediamine tetra-acetic acid cleaning, the cavities were filled with ProRoot Endo Sealer, AH Plus Jet or Pulp Canal Sealer. After setting, half of the cavities were tested with a fibre-optic light-illuminated push-out testing device. The rest were immersed in SBF for 4 weeks before push-out evaluation. Failure modes were examined with stereomicroscopy and field emission (FE)-scanning electron microscopy. Results Location of the Sealer-filled cavities did not affect push-out strengths. ProRoot Endo Sealer exhibited higher push-out strengths than the other two Sealers particularly after SBF storage (P < 0.001). Failure modes were predominantly adhesive and mixed for Pulp Canal Sealer and AH Plus Jet, and predominantly cohesive for ProRoot Endo Sealer. Spherical amorphous calcium phosphate-like phases that spontaneously transformed into apatite-like phases were seen in the fractured specimens of ProRoot Endo Sealer after SBF storage. Conclusions When tested in bulk without a main core, both ‘Sealer type’ and ‘SBF storage’ were significant in affecting push-out results. The ProRoot Endo Sealer demonstrated the presence of spherical amorphous calcium phosphate-like phases and apatite-like phases (i.e. ex vivo bioactivity) after SBF storage.
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in vitro cytotoxicity evaluation of a self adhesive methacrylate resin based root canal Sealer
Journal of Endodontics, 2008Co-Authors: Luigi Pinna, Bradford P. Huffman, Martha G Brackett, Petra E Lockwood, Elisabetta Cotti, Claudia Dettori, David H PashleyAbstract:Abstract This study compared the cytotoxicity of MetaSEAL (Parkell Inc, Farmington, NY), a methacrylate resin-based Sealer with an epoxy resin-based (AH Plus Jet; Dentsply Caulk, Milford, DE) and a zinc oxide–eugenol-based Sealer (Pulp Canal Sealer; SybronEndo, Orange, CA). Five-millimeter diameter disks prepared from the respective Sealer and disks prepared from Teflon (negative control) and polymethyl methacrylate (positive control) were placed in direct contact with a rat osteosarcoma (ROS) 17/2.8 rat osteoblast-like cell line at six intervals after setting completely at 72 hours and for 5 succeeding weeks after the disks were immersed in simulated body fluid. Succinate dehydrogenase activity was evaluated by using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay. All Sealers exhibited severe toxicity at 72 hours, after which toxicity decreased gradually over the experimental period except for Pulp Canal Sealer, which remained severely toxic. MetaSEAL was more toxic than AH Plus Jet during the first week. Both were similar to the toxicity profile of the positive control after the first week, which was probably diffusion controlled.
