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Muhammad Farooq - One of the best experts on this subject based on the ideXlab platform.
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Role of melatonin Seed Priming on antioxidant enzymes and biochemical responses of Carthamus tinctorius L. under drought stress conditions
'Elsevier BV', 2021Co-Authors: Muhammad Farooq, Majid Amini Dehaghi, Łukasz Wojtyla, Keyvan Maleki, Siavash HeshmatiAbstract:Drought has profound effects on productivity of crops in arid and semi- arid regions causing deleterious effects on the functions of crops leading to oxidative stress. In the present study, extensive melatonin Seed Priming experiments were conducted to assess the antioxidant enzymes and biochemical responses of fresh and old primed Seeds of safflower under drought conditions. Safflower Seeds were soaked in water (hydroPriming) or melatonin (0.1 and 0.5 mM) for 6 h. The lipid peroxidation was significantly decreased by melatonin-Seed Priming. Such effects were more prominent in a plant grown from fresh Seeds compared with plants from old Seeds. The antioxidant enzymes varied in activity under drought condition. Our results demonstrated that the response to melatonin Seed Priming was dose-dependent. In addition, the results of this study indicated that the stability of safflower improved by melatonin because the membranes remain intact or sustained a little injury and antioxidant enzymes work properly. This is the first report on the effect of melatonin-Seed Priming on field condition; it also provides evidence that melatonin could improve the tolerance of safflower to drought
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INTERNATIONAL JOURNAL OF AGRICULTURE & BIOLOGY Full Length Article Boron Seed Priming Improves the Seedling Emergence, Growth, Grain Yield and Grain Biofortification of Bread Wheat
2020Co-Authors: Saba Iqbal, Muhammad Farooq, Sardar Alam Cheema, Irfan AfzalAbstract:To cite this paper: Iqbal, S., M. Farooq, S.A. Cheema and I. Afzal, 2017. Boron Seed Priming improves the Seedling emergence, growth, grain yield and grain biofortification of bread wheat. were better than other treatments of both B sources in improving germination and early Seedling growth. In second experiment, wheat Seeds primed with 0.01 and 0.05 M B solutions of both sources of B were sown in sand filled small pots. Seed Priming with 0.01 M B solution of borax was better than other treatments in improving the germination and Seedling growth. In third experiment, wheat Seeds primed with 0.01 and 0.05 M B solution of borax only were sown. Seed Priming with 0.01 M B solution increased the grain yield by 64% over control; however, increase in grain B contents was 27%. In conclusion, wheat Seeds may be primed with 0.01 M B using borax to improve the grain yield and grain B contents in wheat
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improving the productivity and profitability of late sown chickpea by Seed Priming
International Journal of Plant Production, 2019Co-Authors: Muhammad Farooq, Mubshar Hussain, Muhammad Imran, Imran Ahmad, Muhammad Atif, Salem S AlghamdiAbstract:The delayed planting suppresses germination, growth and productivity of chickpea due to low temperature. Seed Priming may improve the germination and growth of chickpea; however, no study has reported the effect of Seed Priming techniques on germination indices, growth and productivity of late-sown chickpea. This study was conducted to evaluate the influence of Seed Priming in improving performance of late sown chickpea. Chickpea Seeds were subjected to on-farm Priming, hydroPriming and osmoPriming (CaCl2). In experiment I, Seeds of chickpea cultivars Punjab-2008 and Thal-2006 were sown in soil filled pots. Seed Priming improved crop stand establishment and Seedling dry weight owing to Priming-induced improvement in sugars’ metabolism. In the second experiment, primed and untreated Seeds of both chickpea cultivars were sown on Dec 03 and 18, and Jan 03 at Faisalabad and Multan, Pakistan during three growing seasons. Delay in planting decreased the germination indices, crop stand, growth and yield at both sites during all 3 years. Seed Priming improved crop stand and growth of both chickpea cultivars resulting in increase in grain yield and net economic returns, and osmoPriming was the most effective. Chickpea cultivars differed for yield and net economic returns and Punjab-2008 had better grain yield and economic returns compared to Thal-2006. Therefore, chickpea cultivar Punjab-2008 should be planted after osmoPriming to harvest better yield and profitability from late sown chickpea.
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Seed Priming of zn with endophytic bacteria improves the productivity and grain biofortification of bread wheat
European Journal of Agronomy, 2018Co-Authors: Abdul Rehman, Muhammad Farooq, Ahmad Nawaz, Muhammad Naveed, Babar ShahzadAbstract:Abstract Zinc (Zn) deficiency hampers the crop yield, nutritional quality and ultimately the human health. Plant growth-promoting endophyte transform unavailable Zn to available form for plant uptake which enhance the plant Zn status and growth. Therefore, a two year field study during 2013–14 and 2014–15 was conducted to evaluate the interactive effect of endophytic bacteria and Zn application methods for improving the productivity and grain biofortification of wheat. Zinc was applied to two wheat cultivars viz. Lasani-2008 (LS-2008) and Faisalabad-2008 (FSD-2008) as soil application (10 kg ha−1), foliar application (0.025 M), Seed Priming (0.5 M) and Seed coating (1.25 g kg−1 Seed), while hydroprimed Seeds were taken as control. Endophytic Zn solubilizing bacterial strain viz. Pseudomonas sp. MN12 was used in combination with different Zn application methods. Results revealed that Zn application through any method improved the grain yield and grain biofortification of bread wheat. Maximum improvement of 27.1% in grain yield was recorded when Zn was applied in combination with strain MN12 through Seed Priming technique against control. However, soil and foliar application of Zn with and without Pseudomonas sp. MN12 resulted in highest improvement in protein content, Zn concentration in whole grain, embryo, aleurone and endosperm of wheat than control. Moreover, Zn application improved the bioavailable Zn as minimum phytate concentration, phytate/Zn molar ratio and maximum bioavailable Zn was recorded for soil (43.9%) and foliar application (45.6%) of Zn + MN12, respectively. Nevertheless, highest net economic return and marginal rate of return with high benefit cost ratio (BCR) was recorded for Seed Priming with Zn + MN12. Furthermore, maximum grain yield, grain Zn concentration with high economic return was recorded for FSD-2008. In crux, Zn may be applied through Seed Priming in combination with Pseudomonas sp. MN12 to improve the productivity and grain biofortification of wheat.
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Seed Priming with sorghum extracts and benzyl aminopurine improves the tolerance against salt stress in wheat triticum aestivum l
Physiology and Molecular Biology of Plants, 2018Co-Authors: Muhammad Farooq, Ali Ahsan Bajwa, Ahmad NawazAbstract:Salt stress impedes the productivity of wheat (Triticum aestivum L.) in many parts of the world. This study evaluated the potential role of benzyl aminopurine (BAP) and sorghum water extract (SWE) in improving the wheat performance under saline conditions. Seeds were primed with BAP (5 mg L−1), SWE (5% v/v), BAP + SWE, and distilled water (hydroPriming). Soil filled pots maintained at the soil salinity levels of 4 and 10 dS m−1 were used for the sowing of primed and non-primed Seeds. Salt stress suppressed the wheat growth; Seed Priming treatments significantly improved the wheat growth under optimal and suboptimal conditions. Total phenolics, total soluble sugars and proteins, α-amylase activity, chlorophyll contents, and tissue potassium ion (K+) contents were increased by Seed Priming under salt stress; while, tissue sodium ion (Na+) contents were decreased. Seed Priming with SWE + BAP was the most effective in this regard. Under salt stress, the tissue Na+ contents were reduced by 5.78, 28.3, 32.2, 36.7% by hydroPriming, Seed Priming with SWE, Seed Priming with BAP, and Seed Priming with SWE + BAP, respectively over the non-primed control. Effectiveness of Seed Priming techniques followed the order SWE + BAP > BAP > SWE > HydroPriming. In conclusion, Seed Priming with SWE + BAP may be opted to improve the tolerance against salt stress in wheat.
Lixiao Nie - One of the best experts on this subject based on the ideXlab platform.
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physiological and biochemical mechanisms of Seed Priming induced chilling tolerance in rice cultivars
Frontiers in Plant Science, 2016Co-Authors: Saddam Hussain, Fahad Khan, Hafiz Athar Hussain, Lixiao NieAbstract:Rice belongs to tropical and subtropical environments and is extremely sensitive to chilling stress particularly during emergence and early stages of Seedling development. Seed Priming can be a good approach to enhance rice germination and stand establishment under chilling stress. The present study examined the role of different Seed Priming techniques viz., hydroPriming, osmoPriming, redox Priming, chemical Priming, and hormonal Priming, in enhancing the chilling tolerance in rice. The most effective reagents and their pre-optimized concentrations based on preliminary experiments were used in this study. Two different rice cultivars were sown under chilling stress (18˚C) and normal temperatures (28˚C) in separate growth chambers. A non-primed control treatment was also maintained for comparison. Chilling stress caused erratic and delayed germination, poor Seedling growth, reduced starch metabolism and lower respiration rate, while higher lipid peroxidation and hydrogen peroxide accumulation in rice Seedlings of both cultivars. Nevertheless, all the Seed Priming treatments effectively alleviated the negative effects of chilling stress. In addition, Seed Priming treatments triggered the activities of superoxide dismutase, peroxidase, and catalase, and enhanced the accumulations of glutathione and free proline in rice Seedlings, which suggests that these measures help prevent the rice Seedlings from chilling induced oxidative stress. Chemical Priming with selenium and hormonal Priming with salicylic acid remained more effective treatments for both rice cultivars under chilling stress than all other Priming treatments. The better performance and greater tolerance of primed rice Seedlings was associated with enhanced starch metabolism, high respiration rate, lower lipid peroxidation, and strong antioxidative defense system under chilling stress.
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benefits of rice Seed Priming are offset permanently by prolonged storage and the storage conditions
Scientific Reports, 2015Co-Authors: Saddam Hussain, Fahad Khan, Manman Zheng, Abdul Khaliq, Shah Fahad, Shaobing Peng, Jianliang Huang, Kehui Cui, Lixiao NieAbstract:Seed Priming is a commercially successful practice, but reduced longevity of primed Seeds during storage may limit its application. We established a series of experiments on rice to test: (1) whether prolonged storage of primed and non-primed rice Seeds for 210 days at 25°C or -4°C would alter their viability, (2) how long primed rice Seed would potentially remain viable at 25°C storage, and (3) whether or not post-storage treatments (re-Priming or heating) would reinstate the viability of stored primed Seeds. Two different rice cultivars and three Priming agents were used in all experiments. Prolonged storage of primed Seeds at 25°C significantly reduced the germination (>90%) and growth attributes (>80%) of rice compared with un-stored primed Seeds. However, such negative effects were not observed in primed Seeds stored at -4°C. Beneficial effects of Seed Priming were maintained only for 15 days of storage at 25°C, beyond which the performance of primed Seeds was worse even than non-primed Seeds. The deteriorative effects of 25°C storage were related with hampered starch metabolism in primed rice Seeds. None of the post-storage treatments could reinstate the lost viability of primed Seeds suggesting that Seeds become unviable by prolonged post-Priming storage at 25°C.
Ahmad Nawaz - One of the best experts on this subject based on the ideXlab platform.
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Seed Priming of zn with endophytic bacteria improves the productivity and grain biofortification of bread wheat
European Journal of Agronomy, 2018Co-Authors: Abdul Rehman, Muhammad Farooq, Ahmad Nawaz, Muhammad Naveed, Babar ShahzadAbstract:Abstract Zinc (Zn) deficiency hampers the crop yield, nutritional quality and ultimately the human health. Plant growth-promoting endophyte transform unavailable Zn to available form for plant uptake which enhance the plant Zn status and growth. Therefore, a two year field study during 2013–14 and 2014–15 was conducted to evaluate the interactive effect of endophytic bacteria and Zn application methods for improving the productivity and grain biofortification of wheat. Zinc was applied to two wheat cultivars viz. Lasani-2008 (LS-2008) and Faisalabad-2008 (FSD-2008) as soil application (10 kg ha−1), foliar application (0.025 M), Seed Priming (0.5 M) and Seed coating (1.25 g kg−1 Seed), while hydroprimed Seeds were taken as control. Endophytic Zn solubilizing bacterial strain viz. Pseudomonas sp. MN12 was used in combination with different Zn application methods. Results revealed that Zn application through any method improved the grain yield and grain biofortification of bread wheat. Maximum improvement of 27.1% in grain yield was recorded when Zn was applied in combination with strain MN12 through Seed Priming technique against control. However, soil and foliar application of Zn with and without Pseudomonas sp. MN12 resulted in highest improvement in protein content, Zn concentration in whole grain, embryo, aleurone and endosperm of wheat than control. Moreover, Zn application improved the bioavailable Zn as minimum phytate concentration, phytate/Zn molar ratio and maximum bioavailable Zn was recorded for soil (43.9%) and foliar application (45.6%) of Zn + MN12, respectively. Nevertheless, highest net economic return and marginal rate of return with high benefit cost ratio (BCR) was recorded for Seed Priming with Zn + MN12. Furthermore, maximum grain yield, grain Zn concentration with high economic return was recorded for FSD-2008. In crux, Zn may be applied through Seed Priming in combination with Pseudomonas sp. MN12 to improve the productivity and grain biofortification of wheat.
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Seed Priming with sorghum extracts and benzyl aminopurine improves the tolerance against salt stress in wheat triticum aestivum l
Physiology and Molecular Biology of Plants, 2018Co-Authors: Muhammad Farooq, Ali Ahsan Bajwa, Ahmad NawazAbstract:Salt stress impedes the productivity of wheat (Triticum aestivum L.) in many parts of the world. This study evaluated the potential role of benzyl aminopurine (BAP) and sorghum water extract (SWE) in improving the wheat performance under saline conditions. Seeds were primed with BAP (5 mg L−1), SWE (5% v/v), BAP + SWE, and distilled water (hydroPriming). Soil filled pots maintained at the soil salinity levels of 4 and 10 dS m−1 were used for the sowing of primed and non-primed Seeds. Salt stress suppressed the wheat growth; Seed Priming treatments significantly improved the wheat growth under optimal and suboptimal conditions. Total phenolics, total soluble sugars and proteins, α-amylase activity, chlorophyll contents, and tissue potassium ion (K+) contents were increased by Seed Priming under salt stress; while, tissue sodium ion (Na+) contents were decreased. Seed Priming with SWE + BAP was the most effective in this regard. Under salt stress, the tissue Na+ contents were reduced by 5.78, 28.3, 32.2, 36.7% by hydroPriming, Seed Priming with SWE, Seed Priming with BAP, and Seed Priming with SWE + BAP, respectively over the non-primed control. Effectiveness of Seed Priming techniques followed the order SWE + BAP > BAP > SWE > HydroPriming. In conclusion, Seed Priming with SWE + BAP may be opted to improve the tolerance against salt stress in wheat.
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Seed Priming improves stand establishment and productivity of no till wheat grown after direct Seeded aerobic and transplanted flooded rice
European Journal of Agronomy, 2016Co-Authors: Muhammad Farooq, Ahmad Nawaz, Riaz Ahmad, S M A Basra, Rattan LalAbstract:Abstract No tillage (NT) in wheat (Triticum aestivum L.) offers a pragmatic option for resolving the time and edaphic conflicts in rice (Oryza sativa L.)–wheat cropping system (RWS). However, poor stand establishment is an issue in NT wheat, which adversely affects crop growth, grain yield, and profitability. Therefore, a 2-year field study was conducted to assess the potential role of Seed Priming in improving the stand establishment, grain yield, water productivity and profitability of NT and plough till (PT) wheat grown after direct Seeded aerobic (conservation) and puddled transplanted flooded (conventional) rice-based systems. For Seed Priming, wheat Seeds were soaked in aerated water (hydroPriming) or solution of CaCl2 (ψs −1.25 MPa; osmoPriming) for 12 h, and non-primed Seeds were used as control. After harvest of rice, grown as direct Seeded aerobic and puddled transplanted flooded crop, primed and non-primed wheat Seeds were sown following NT and PT. In both years, stand establishment of NT wheat after direct Seeded aerobic and puddled transplanted flooded rice was impeded. Nonetheless, Seed Priming improved the stand establishment which was visible through earliness and better uniformity of Seedling emergence. Overall, primed Seeds completed 50% emergence in 6.4 days, against 7.8 days taken by non-primed Seeds in NT wheat. The highest emergence index (41.7) was recorded in primed Seeds versus 32.0 for non-primed Seeds. Improved stand establishment enhanced growth, grain yield, water productivity and profitability in NT wheat. In this regard, osmoPriming was the most effective, and produced grain yield of 4.5 Mg ha−1 against 3.8 Mg ha−1 for non-primed Seeds in NT wheat. Water productivity of the NT wheat grown from osmoprimed Seeds was 8.72 kg ha−1 mm−1 while that from non-primed Seeds was 7.21 kg ha−1 mm−1. Among the RWSs, the maximum wheat biomass was produced with PT after direct Seeded aerobic rice. However, grain yield, water productivity, and profitability were the highest in NT wheat following direct Seeded aerobic rice. Wheat yields grown after direct Seeded aerobic rice and transplanted flooded rice were 4.4 and 4.2 Mg ha−1 respectively. Planting NT wheat after direct Seeded aerobic rice provided the highest system productivity (1.80) than other RWSs. Thus, Seed Priming is a viable option to improve the stand establishment, grain yield, water productivity and profitability of NT wheat in the RWS. Nonetheless, osmoPriming was a better option than hydroPriming in this regard.
Hafeez Ur Rehman - One of the best experts on this subject based on the ideXlab platform.
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Seed Priming improves early Seedling vigor growth and productivity of spring maize
Journal of Integrative Agriculture, 2015Co-Authors: Hafeez Ur Rehman, Muhammad Farooq, Shahzad Ma Basra, Irfan Afzal, Hassan Iqbal, Abdul Wakeel, Ning WangAbstract:Abstract Potential of Seed Priming treatments in improving the performance of early planted maize was evaluated against timely planting. Seeds of maize hybrid FH-810 were soaked in water (hydroPriming), CaCl2 (2.2%, osmoPriming), moringa leaf extracts (MLE 3.3%, osmoPriming) and salicylic acid (SA, 50 mg L−1, hormonal Priming) each for 18 h. Untreated and hydroprimed Seeds were taken as control. Seeds primed with SA took less time in emergence and had high vigor in early planted maize. Amongst treatments, hormonal Priming, reduced the electrical conductivity, increased the leaf relative and chlorophyll contents followed by osmoPriming with CaCl2 at Seedling stage. Likewise, plant height, grain rows and 1 000-grain weight, grain and biological yield and harvest index were also improved by Seed Priming; however hormonal Priming and osmoPriming with MLE were more effective in this regard. Improved yield performance by hormonal Priming or osmoPriming with MLE in early planting primarily owed to increased leaf area index, crop growth and net assimilation rates, and maintenance of green leaf area at maturity. In conclusion, osmoPriming with MLE and hormonal Priming with SA were the most economical treatments in improving productivity of early planted spring maize through stimulation of early Seedling growth at low temperature.
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influence of Seed Priming on performance and water productivity of direct Seeded rice in alternating wetting and drying
Rice Science, 2015Co-Authors: Hafeez Ur Rehman, Shahzad Ma Basra, Muhammad Kamran, Irfan Afzal, Muhammad FarooqAbstract:Abstract Direct Seeded rice is promising alternative to traditional transplanting, but requires appropriate crop and water management to maintain yield performance and achieve high water productivity. Present study evaluated the effect of Seed Priming and irrigation on crop establishment, tillering, agronomic traits, paddy yield, grain quality and water productivity of direct Seeded rice in alternate wetting and drying (DSR-AWD) in comparison with direct Seeded rice at field capacity (DSR-FC). Seed Priming treatments were osmo-Priming with KCl (2.2%), CaCl 2 (2.2%) and moringa leaf extracts (MLE, 3.3%) including hydro-Priming as control. Among the treatments, Seed osmo-primed with MLE emerged earlier and had higher final emergence, followed by osmo-Priming with CaCl 2 . Tillering emergence rate and number of tillers per plant were the highest for Seed Priming with CaCl 2 in DSR-AWD. Total productive and non-productive tillers, panicle length, biological and grain yields, harvest index were highest for Seed Priming with MLE or CaCl 2 in DSR-AWD. Similarly, grain quality, estimated in terms of normal grains, abortive and chalky grains, was also the highest in DSR-AWD with MLE osmo-Priming. Benefit cost ratio and water productivity was also the highest in DSR-AWD for Seed Priming with MLE. In conclusion, Seed Priming with MLE or CaCl 2 can be successfully employed to improve the direct Seeded rice performance when practiced with alternate wetting and drying irrigation.
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chilling tolerance in hybrid maize induced by Seed Priming with salicylic acid
Journal of Agronomy and Crop Science, 2008Co-Authors: Muhammad Farooq, Tariq Aziz, S M A Basra, M A Cheema, Hafeez Ur RehmanAbstract:The optimum temperature for maize germination is between 25 and 28 °C. Poor and erratic germination at suboptimal temperature is the most important hindrance in its early sowing. This study was conducted to induce chilling tolerance in hybrid maize (Zea mays L.) by Seed Priming with salicylic acid (SA) and to unravel the background biochemical basis. For Seed Priming, maize hybrid (Hycorn 8288) Seeds were soaked in 50, 100 and 150 ppm (mg l -1 ) aerated solutions of SA for 24 h and were dried back. Treated and untreated Seeds were sown at 27 °C (optimal temperature) and at 15 °C (chilling stress) under controlled conditions. Performance of maize Seedlings was hampered under chilling stress. But Seed Priming with SA improved the Seedling emergence, root and shoot length, Seedling fresh and dry weights, and leaf and root score considerably compared with control both at optimal and chilling temperatures. However, Priming in 50 mg l -1 SA solution was more effective, followed by Priming in 100 mg l -1 SA solution. Seed Priming with SA improved the chilling tolerance in hybrid maize mainly by the activation of antioxidants (including catalase, superoxide dismutase and ascorbate peroxidase). Moreover, maintenance of high tissue water contents and reduced membrane permeability also contributed towards chilling tolerance.
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Seed Priming enhances the performance of late sown wheat triticum aestivum l by improving chilling tolerance
Journal of Agronomy and Crop Science, 2008Co-Authors: Muhammad Farooq, S M A Basra, Hafeez Ur Rehman, Basharat Ali SaleemAbstract:In rice-wheat systems, late sowing of wheat is the major reason of low yield. This yield reduction is principally due to lower and erratic germination, and poor crop establishment because of low temperature prevailing. The present study was conducted to explore the possibility of improving late sown wheat performance by Seed Priming techniques. Seed Priming strategies were: on-farm Seed Priming, hydroPriming for 24 h, Seed hardening for 12 h and osmohardening with KCl or CaCl 2 for 12 h. Seed Priming improved emergence, stand establishment, tiller numbers, allometry, grain and straw yield, and harvest index. However, Seed Priming techniques did not affect plant height, number of spikelets, number of grains and 1000 grain weight. Osmohardening with CaCl 2 gave more grain and straw yield and harvest index compared with control and other Priming treatments, followed by osmohardening with KCl and on-farm Seed Priming. Improved yield was attributed principally to better stand establishment and improved number of fertile tillers. Seed Priming techniques can be effectively used to improve the performance of late sown wheat.
Saddam Hussain - One of the best experts on this subject based on the ideXlab platform.
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Seed Priming alters the production and detoxification of reactive oxygen intermediates in rice Seedlings grown under sub optimal temperature and nutrient supply
Frontiers in Plant Science, 2016Co-Authors: Saddam Hussain, Fahad Khan, Lishu Wu, Mingjian GengAbstract:The production and detoxification of reactive oxygen intermediates (ROIs) play an important role in the plant response to nutrient and environmental stresses. The present study demonstrated the behavior of growth, ROIs-production and their detoxification in primed and non-primed rice Seedlings under chilling stress (18˚C) and nitrogen-(N), phosphorus-(P), or potassium-(K) deprivation. The results revealed that chilling stress as well as deprivation of any mineral nutrient severely hampered the Seedling growth of rice, however, Seed Priming treatments (particularly selenium- or salicylic acid-Priming), were effective in enhancing the rice growth under stress conditions. The N-deprivation caused the maximum reduction in shoot growth, while the root growth was only decreased by P- or K-deprivation. Although, N-deprivation enhanced the root length of rice, the root fresh weight was unaffected. Rate of lipid peroxidation as well as the production of ROIs, was generally increased under stress conditions; the K-deprived Seedlings recorded significantly lower production of ROIs than N- or P-deprived Seedlings. The responses of enzymatic and non-enzymatic antioxidants in rice Seedlings to chilling stress were variable with nutrient management regime. All the Seed Priming were found to trigger or at least maintain the antioxidant defense system of rice Seedlings. Notably, the levels of ROIs were significantly reduced by Seed Priming treatments, which were concomitant with the activities of ROIs-producing enzymes (monoamine oxidase and xanthine oxidase), under all studied conditions. Based on these findings, we put forward the hypothesis that along with role of ROIs-scavenging enzymes, the greater tolerance of primed rice Seedlings can also be due to the reduced activity of ROIs-producing enzymes.
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physiological and biochemical mechanisms of Seed Priming induced chilling tolerance in rice cultivars
Frontiers in Plant Science, 2016Co-Authors: Saddam Hussain, Fahad Khan, Hafiz Athar Hussain, Lixiao NieAbstract:Rice belongs to tropical and subtropical environments and is extremely sensitive to chilling stress particularly during emergence and early stages of Seedling development. Seed Priming can be a good approach to enhance rice germination and stand establishment under chilling stress. The present study examined the role of different Seed Priming techniques viz., hydroPriming, osmoPriming, redox Priming, chemical Priming, and hormonal Priming, in enhancing the chilling tolerance in rice. The most effective reagents and their pre-optimized concentrations based on preliminary experiments were used in this study. Two different rice cultivars were sown under chilling stress (18˚C) and normal temperatures (28˚C) in separate growth chambers. A non-primed control treatment was also maintained for comparison. Chilling stress caused erratic and delayed germination, poor Seedling growth, reduced starch metabolism and lower respiration rate, while higher lipid peroxidation and hydrogen peroxide accumulation in rice Seedlings of both cultivars. Nevertheless, all the Seed Priming treatments effectively alleviated the negative effects of chilling stress. In addition, Seed Priming treatments triggered the activities of superoxide dismutase, peroxidase, and catalase, and enhanced the accumulations of glutathione and free proline in rice Seedlings, which suggests that these measures help prevent the rice Seedlings from chilling induced oxidative stress. Chemical Priming with selenium and hormonal Priming with salicylic acid remained more effective treatments for both rice cultivars under chilling stress than all other Priming treatments. The better performance and greater tolerance of primed rice Seedlings was associated with enhanced starch metabolism, high respiration rate, lower lipid peroxidation, and strong antioxidative defense system under chilling stress.
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benefits of rice Seed Priming are offset permanently by prolonged storage and the storage conditions
Scientific Reports, 2015Co-Authors: Saddam Hussain, Fahad Khan, Manman Zheng, Abdul Khaliq, Shah Fahad, Shaobing Peng, Jianliang Huang, Kehui Cui, Lixiao NieAbstract:Seed Priming is a commercially successful practice, but reduced longevity of primed Seeds during storage may limit its application. We established a series of experiments on rice to test: (1) whether prolonged storage of primed and non-primed rice Seeds for 210 days at 25°C or -4°C would alter their viability, (2) how long primed rice Seed would potentially remain viable at 25°C storage, and (3) whether or not post-storage treatments (re-Priming or heating) would reinstate the viability of stored primed Seeds. Two different rice cultivars and three Priming agents were used in all experiments. Prolonged storage of primed Seeds at 25°C significantly reduced the germination (>90%) and growth attributes (>80%) of rice compared with un-stored primed Seeds. However, such negative effects were not observed in primed Seeds stored at -4°C. Beneficial effects of Seed Priming were maintained only for 15 days of storage at 25°C, beyond which the performance of primed Seeds was worse even than non-primed Seeds. The deteriorative effects of 25°C storage were related with hampered starch metabolism in primed rice Seeds. None of the post-storage treatments could reinstate the lost viability of primed Seeds suggesting that Seeds become unviable by prolonged post-Priming storage at 25°C.