The Experts below are selected from a list of 384 Experts worldwide ranked by ideXlab platform

Chedly Abdelly - One of the best experts on this subject based on the ideXlab platform.

  • Nickel tolerance and toxicity mechanisms in the halophyte Sesuvium portulacastrum L. as revealed by Ni localization and ligand environment studies
    Environmental Science and Pollution Research, 2019
    Co-Authors: Emna Fourati, Mariem Wali, Anja Kavcic, Mitja Kelemen, Primož Vavpetic, Primož Pelicon, Katarina Vogel-mikuš, Jana Padežnik Gomilšek, Alojz Kodre, Chedly Abdelly
    Abstract:

    Halophytes are able to tolerate relatively high concentrations of hazardous metals in a growing substrate, what makes them suitable candidates for phytoremediation of metal-contaminated soils. In this work, we aimed to study the physiological responses of the halophyte Sesuvium portulacastrum L. to Ni, with main focus on Ni localization, compartmentation and ligand environment, to decipher Ni tolerance and toxicity mechanisms. Seedlings were grown in hydroponic nutrient solution containing 0, 25, 50 and 100 μM Ni as NiCl_2 for 3 weeks. Ni localization in leaves was assessed by micro-proton-induced X-ray emission (micro-PIXE). Ni ligand environment was studied by Ni K-edge X-ray absorption near edge structure (XANES). In addition, Ni-soluble, weakly bound/exchangeable and insoluble leaf tissue fractions were determined by sequential extraction. Results show that S. portulacastrum is able to tolerate up to ~ 500 μg g^−1 dry weight (DW) of Ni in the shoots without significant growth reduction. At higher Ni concentrations (> 50 μM Ni in nutrient solution), chloroses were observed due to the accumulation of Ni in photosynthetically active chlorenchyma as revealed by micro-PIXE. Water storage tissue represented the main pool for Ni storage. Incorporation of Ni into Ca-oxalate crystals was also observed in some specimens, conferring tolerance to high leaf Ni concentrations. The majority of Ni (> 70%) was found in soluble tissue fraction. Ni K XANES revealed Ni bound mainly to O- (55%) and N-ligands (45%). Ni toxicity at higher Ni levels was associated with Ni binding to amino groups of proteins in cytosol of chlorenchyma and increased level of lipid peroxidation. Proline levels also increased at high Ni exposures and were associated with Ni-induced oxidative stress and alteration of water regime.

  • physiological response and mineral elements accumulation pattern in Sesuvium portulacastrum l subjected in vitro to nickel
    Chemosphere, 2019
    Co-Authors: Emna Fourati, Chedly Abdelly, Katarina Vogelmikus, Taoufik Bettaieb, Anja Kavcic, Mitja Kelemen, Primož Vavpetic, Primož Pelicon, Tahar Ghnaya
    Abstract:

    Abstract Sesuvium portulacastrum, a halophyte with high tolerance to heavy metals like Cd, Pb and Ni is considered for phytoremediation of metal contaminated saline soils. The tolerance to a selected metal ion could, by hypothesis, be stimulated through in vitro adaptation and regeneration of the plant. Seedlings obtained by in vitro micro-propagation, were exposed to 0, 25 and 50 μM Ni, as NiCl2, in agar-based medium for 30 days. Growth parameters, plant water content, the concentration of photosynthetic pigments, proline and malondialdehyde (MDA) concentrations were determined. Nickel and nutrients distribution in leaves was studied by micro-Proton-Induced-X-ray-Emission (μ-PIXE). The results showed that Ni was mainly accumulated in vascular bundles, next in water storage tissues and chlorenchyma. Ni concentrations in chlorenchyma increased with increasing Ni in culturing medium, in direct relation to decrease of photosynthetic pigments and increase of oxidative stress. As compared to control plants, Ni induced substantial increase in MDA and proline accumulation. Plants exposed to 50 μM Ni accumulated up to 650 μg g−1 of Ni in the shoots, exhibiting chlorosis and necrosis and a drastically reduced plant growth. Perturbations in uptake and distribution of nutrients were observed, inducing mineral deficiency, probably through membrane leakage. The mineral nutrient disturbances induced by Ni could be highly implicated in the restriction of S. portulacastrum development under the acute 50 μM Ni level.

  • effects of salt treatment on growth lipid membrane peroxidation polyphenol content and antioxidant activities in leaves of Sesuvium portulacastrum l
    Arid Land Research and Management, 2017
    Co-Authors: Inès Slama, Rawya Mrabet, Riadh Ksouri, Ons Talbi, Ahmed Debez, Chedly Abdelly
    Abstract:

    ABSTRACTChanges in growth, leaf contents of proline, oxidative stress-related parameters, and phenolic compounds, and antioxidant activities were investigated in the halophyte species Sesuvium portulacastrum L. under saline conditions. Rooted cuttings were individually cultivated in sandy soil. After five weeks of pre-treatment, seedlings were submitted during one month to different salt concentrations ranging from 0 to 800 mM NaCl. The plant growth was significantly improved by salt at 200–600 mM concentration. This trend was associated with (i) the stimulation of photosynthetic activity, (ii) the protection of membrane integrity (leaf MDA content 50% lower than the control), and (iii) higher total antioxidant activity, especially at 400 mM NaCl. At this salt concentration plants accumulated high contents of proline, polyphenols, antocyanins, and carotenoids. These compounds could be implied in the protection of the photosynthetic system and in the improvement of growth. Exposure to 800 mM NaCl impaired ...

  • Cadmium hampers salt tolerance of Sesuvium portulacastrum
    Plant physiology and biochemistry : PPB, 2017
    Co-Authors: Mariem Wali, Chedly Abdelly, Tahar Ghnaya, Soledad Martos, Laura Pérez-martín, Charlotte Poschenrieder, Benet Gunsé
    Abstract:

    Abstract It is well known that salinity reduces cadmium toxicity in halophytes. However, the possible interference of Cd with the mechanisms of salt tolerance is poorly explored. The aim of this study was to see whether Cd affects salt tolerance mechanisms in the halophyte Sesuvium portulacastrum . S. portulacastrum plants obtained from cuttings were grown in hydroponics for 3 weeks and then exposed to low (0.09 mM) or moderate (200 mM) NaCl concentrations, alone or in combination with 25 μM CdCl 2 . Microscopy observation revealed two strategies of salt tolerance: euhalophytism and secretion of salt by bladder cells. Cadmium exposure hardly influenced the total leaf Na + concentrations. However, Cd supply delayed the salt–induced upregulation of AHA1 (plasma membrane H + -ATPase 1) and SOS1 (plasma membrane Na + transporter “Salt Overly Sensitive 1”), genes that are essential for salt tolerance. Moreover, Cd induced the activation of BADH , coding for betaine aldehyde dehydrogenase, indicating enhanced osmotic stress due to Cd. Sodium-green fluorescence in protoplasts from plants grown with low or high NaCl, alone or in combination with Cd, revealed higher Na + concentrations in the cytoplasm of Cd-exposed plants. Taken together the results indicate interference of Cd with salt tolerance mechanisms in S. portulacastrum . This may have consequences for the efficient use of halophytes in phytoremediation of Cd-contaminated saline soils.

  • nickel tolerance accumulation and subcellular distribution in the halophytes Sesuvium portulacastrum and cakile maritima
    Plant Physiology and Biochemistry, 2016
    Co-Authors: Emna Fourati, Chedly Abdelly, Mariem Wali, Katarina Vogelmikus, Tahar Ghnaya
    Abstract:

    Abstract It has been shown that halophytes are able to successfully cope with heavy metal toxicity, suggesting their possible use for remediation of metal contaminated soils. In this work, Ni tolerance and accumulation in two halophytes, Sesuvium portulacastrum (L.) L. and Cakile maritima Scop. was investigated. Seedlings of both species were subjected hydroponically during 21 days to 0, 25, 50, and 100 μM of NiCl2. The growth and photosynthesis parameters revealed that S. portulacastrum tolerates Ni better than C. maritima. The photosynthesis activity, chlorophyll content and photosystem II integrity were less impacted in Ni-treated S. portulacastrum as compared to C. maritima, although, Ni accumulated in higher concentrations in the shoots of S. portulacastrum (1050 μg g−1 DW) than in those of C. maritima (550 μg g−1 DW). The subcellular fractionation of Ni in the shoots of both species showed that C. maritima accumulated about 65% of Ni in the soluble fraction, while 28% was associated with the cell walls. In S. portulacastrum 44% of the total cellular Ni was seen in the soluble fraction and 43% was bound to the cell walls. It can be concluded that S. portulacastrum tolerates Ni better than C. maritima, most probably due to a better ability to sequester Ni in the cell walls, restricting its accumulation in the soluble fraction.

Tahar Ghnaya - One of the best experts on this subject based on the ideXlab platform.

  • physiological response and mineral elements accumulation pattern in Sesuvium portulacastrum l subjected in vitro to nickel
    Chemosphere, 2019
    Co-Authors: Emna Fourati, Chedly Abdelly, Katarina Vogelmikus, Taoufik Bettaieb, Anja Kavcic, Mitja Kelemen, Primož Vavpetic, Primož Pelicon, Tahar Ghnaya
    Abstract:

    Abstract Sesuvium portulacastrum, a halophyte with high tolerance to heavy metals like Cd, Pb and Ni is considered for phytoremediation of metal contaminated saline soils. The tolerance to a selected metal ion could, by hypothesis, be stimulated through in vitro adaptation and regeneration of the plant. Seedlings obtained by in vitro micro-propagation, were exposed to 0, 25 and 50 μM Ni, as NiCl2, in agar-based medium for 30 days. Growth parameters, plant water content, the concentration of photosynthetic pigments, proline and malondialdehyde (MDA) concentrations were determined. Nickel and nutrients distribution in leaves was studied by micro-Proton-Induced-X-ray-Emission (μ-PIXE). The results showed that Ni was mainly accumulated in vascular bundles, next in water storage tissues and chlorenchyma. Ni concentrations in chlorenchyma increased with increasing Ni in culturing medium, in direct relation to decrease of photosynthetic pigments and increase of oxidative stress. As compared to control plants, Ni induced substantial increase in MDA and proline accumulation. Plants exposed to 50 μM Ni accumulated up to 650 μg g−1 of Ni in the shoots, exhibiting chlorosis and necrosis and a drastically reduced plant growth. Perturbations in uptake and distribution of nutrients were observed, inducing mineral deficiency, probably through membrane leakage. The mineral nutrient disturbances induced by Ni could be highly implicated in the restriction of S. portulacastrum development under the acute 50 μM Ni level.

  • Cadmium hampers salt tolerance of Sesuvium portulacastrum
    Plant physiology and biochemistry : PPB, 2017
    Co-Authors: Mariem Wali, Chedly Abdelly, Tahar Ghnaya, Soledad Martos, Laura Pérez-martín, Charlotte Poschenrieder, Benet Gunsé
    Abstract:

    Abstract It is well known that salinity reduces cadmium toxicity in halophytes. However, the possible interference of Cd with the mechanisms of salt tolerance is poorly explored. The aim of this study was to see whether Cd affects salt tolerance mechanisms in the halophyte Sesuvium portulacastrum . S. portulacastrum plants obtained from cuttings were grown in hydroponics for 3 weeks and then exposed to low (0.09 mM) or moderate (200 mM) NaCl concentrations, alone or in combination with 25 μM CdCl 2 . Microscopy observation revealed two strategies of salt tolerance: euhalophytism and secretion of salt by bladder cells. Cadmium exposure hardly influenced the total leaf Na + concentrations. However, Cd supply delayed the salt–induced upregulation of AHA1 (plasma membrane H + -ATPase 1) and SOS1 (plasma membrane Na + transporter “Salt Overly Sensitive 1”), genes that are essential for salt tolerance. Moreover, Cd induced the activation of BADH , coding for betaine aldehyde dehydrogenase, indicating enhanced osmotic stress due to Cd. Sodium-green fluorescence in protoplasts from plants grown with low or high NaCl, alone or in combination with Cd, revealed higher Na + concentrations in the cytoplasm of Cd-exposed plants. Taken together the results indicate interference of Cd with salt tolerance mechanisms in S. portulacastrum . This may have consequences for the efficient use of halophytes in phytoremediation of Cd-contaminated saline soils.

  • nickel tolerance accumulation and subcellular distribution in the halophytes Sesuvium portulacastrum and cakile maritima
    Plant Physiology and Biochemistry, 2016
    Co-Authors: Emna Fourati, Chedly Abdelly, Mariem Wali, Katarina Vogelmikus, Tahar Ghnaya
    Abstract:

    Abstract It has been shown that halophytes are able to successfully cope with heavy metal toxicity, suggesting their possible use for remediation of metal contaminated soils. In this work, Ni tolerance and accumulation in two halophytes, Sesuvium portulacastrum (L.) L. and Cakile maritima Scop. was investigated. Seedlings of both species were subjected hydroponically during 21 days to 0, 25, 50, and 100 μM of NiCl2. The growth and photosynthesis parameters revealed that S. portulacastrum tolerates Ni better than C. maritima. The photosynthesis activity, chlorophyll content and photosystem II integrity were less impacted in Ni-treated S. portulacastrum as compared to C. maritima, although, Ni accumulated in higher concentrations in the shoots of S. portulacastrum (1050 μg g−1 DW) than in those of C. maritima (550 μg g−1 DW). The subcellular fractionation of Ni in the shoots of both species showed that C. maritima accumulated about 65% of Ni in the soluble fraction, while 28% was associated with the cell walls. In S. portulacastrum 44% of the total cellular Ni was seen in the soluble fraction and 43% was bound to the cell walls. It can be concluded that S. portulacastrum tolerates Ni better than C. maritima, most probably due to a better ability to sequester Ni in the cell walls, restricting its accumulation in the soluble fraction.

  • cd and ni transport and accumulation in the halophyte Sesuvium portulacastrum implication of organic acids in these processes
    Frontiers in Plant Science, 2015
    Co-Authors: Mejda Mnasri, Chedly Abdelly, Hanen Zaier, Stanley Lutts, Rim Ghabriche, Emna Fourati, Kebba Sabally, Suzelle Barrington, Tahar Ghnaya
    Abstract:

    The implication of organic acids in Cd and Ni translocation was studied in the halophyte species Sesuvium portulacastrum. Citric, fumaric, malic, and ascorbic acids were separated and quantified by HPLC technique in shoots, roots and xylem saps of plants grown on nutrient solutions added with 50 μM Cd, 100 μM Ni and the combination of 50 μM Cd + 100 μM Ni. Results showed that Cd had no significant impact on biomass production while Ni and the combination of both metals drastically affected plant development. Cadmium and Ni concentrations in tissues and xylem sap were higher in plants subjected to individual metal application than those subjected to the combined effect of Cd and Ni suggesting a possible competition between these metals for absorption. Both metals applied separately or in combination induced an increase in citrate concentration in shoots and xylem sap but a decrease of this concentration in the roots. However, a minor relationship was observed between metal application and fumaric, malic, and ascorbic acids. Both observations suggest the implication of citric acid in Cd, Ni translocation and shoot accumulation in S. portulacastrum. The relatively high accumulation of citric acid in xylem sap and shoot of S. portulacastrum could be involved in metal chelation and thus contributes to heavy metal tolerance in this species.

  • nacl alleviates cd toxicity by changing its chemical forms of accumulation in the halophyte Sesuvium portulacastrum
    Environmental Science and Pollution Research, 2015
    Co-Authors: Mariem Wali, Chedly Abdelly, Charlotte Poschenrieder, Rim Ghabriche, Emna Fourati, Nizar Hmaeid, Tahar Ghnaya
    Abstract:

    It has previously been shown that certain halophytes can grow and produce biomass despite of the contamination of their saline biotopes with toxic metals. This suggests that these plants are able to cope with both salinity and heavy metal constraints. NaCl is well tolerated by halophytes and apparently can modulate their responses to Cd. However, the underlying mechanisms remain unclear. This study explores the impact of NaCl on growth, Cd accumulation, and Cd speciation in tissues of the halophyte Sesuvium portulacastrum. Seedlings of S. portulacastrum were exposed during 1 month to 0, 25, and 50 μM Cd combined with low salinity (LS, 0.09 mM NaCl) or high salinity (HS, 200 mM NaCl) levels. Growth parameters and total tissue Cd concentrations were determined, in leaves, stems, and root. Moreover, Cd speciation in these organs was assessed by specific extraction procedures. Results showed that, at LS, Cd induced chlorosis and necrosis and drastically reduced plant growth. However, addition of 200 mM NaCl to Cd containing medium alleviated significantly Cd toxicity symptoms and restored plant growth. NaCl reduced the concentration of Cd in the shoots; nevertheless, due to maintenance of higher biomass under HS, the quantity of accumulated Cd was not modified. NaCl modified the chemical form of Cd in the tissues by increasing the proportion of Cd bound to pectates, proteins, and chloride suggesting that this change in speciation is involved in the positive impact of NaCl on Cd tolerance. We concluded that the tolerance of S. portulacastrum to Cd was enhanced by NaCl. This effect is rather governed by the modification of the speciation of the accumulated Cd than by the reduction of Cd absorption and translocation.

Penna Suprasanna - One of the best experts on this subject based on the ideXlab platform.

  • exposure to nacl enhances cd2 biosorption potential of Sesuvium portulacastrum l
    Environmental Technology and Innovation, 2021
    Co-Authors: T. D. Nikam, Jayant V Kulkarni, Harshala Parab, Ashish Kumar Srivastava, Sangita D Kumar, Mahesh Borde, Penna Suprasanna
    Abstract:

    Abstract Cadmium (Cd2＋) is a major heavy metal pollutant and it causes many serious effects on the environment. The established Cd2＋ removal methods are expensive and produce secondary waste, while biosorption is a potential alternative. In view of this, Sesuvium portulacastrum (L.) is explored, which is a facultative halophyte and is known to survive under both salt and heavy metal stress conditions. Using the dried powder of Sesuvium biomass, two independent biosorbents were prepared including ConB (grown under control condition) and NaClB (grown under 200 mM NaCl) and their Fourier-transform infrared spectroscopy (FTIR) indicated significant alteration in peak position of various functional groups. The Cd2＋ biosorption capacity in NaClB (306 mg g−1) was found higher than ConB (203 mg g−1). The kinetic and equilibrium studies revealed the contribution of intraparticle diffusion in the biosorption process of both the biosorbents. Further, the higher intraparticle diffusion rate constant indicated the faster biosorption process in NaClB. The experimental data correlated better with Freundlich equilibrium isotherm ( r 2 = 0 . 96 for ConB and r 2 = 0 . 99 for NaClB) than Langmuir, indicating multilayer biosorption and heterogeneity within the biosorbents. Scanning electron microscope (SEM) imaging indicated that the rough surface of ConB changed to smooth after Cd2＋ biosorption, with lesser number of pores. In case of NaClB, Cd2＋ biosorption narrowed the vertical channels. EDX (Energy Dispersive X-ray) spectrum validated the Cd2＋ binding in both ConB and NaClB. Taken together, the study highlighted NaCl-treated Sesuvium as an eco-friendly green biosorbent for Cd2＋ removal from aqueous solutions.

  • copper accumulation and biochemical responses of Sesuvium portulacastrum l
    Materials Today: Proceedings, 2020
    Co-Authors: Vinayak H. Lokhande, Penna Suprasanna, Vikas Yadav Patade, Sudhakar Srivastava, Manoj Shrivastava, Garima Awasthi
    Abstract:

    Abstract Sesuvium portulacastrum (L.) is a halophytic phytoremediator plant with potential for significant accumulation of metals and metalloids. The present work evaluatedthe physiological and biochemical responses of Sesuvium plants to copper (Cu) exposure (100–500 μM) for 30 d in field conditions. Plants demonstrated significant copper accumulation that increased with the increase in Cu concentration of the medium (maximum 254 µg g−1 DW at 500 µM). The root dry weight was not significantly affected at 500 μM while shoot dry weight decreased significantly. Total soluble proteins, photosynthetic pigmentsand malondialdehye (MDA) were declined significantly beyond 100 μM after 30 d. Among metabolites and enzymes of thiol metabolism, total non-protein thiols (NP-SH), γ-glutamylcysteine synthetase and glutathione reductase did not show significant effect while cysteine, serine acetyltransferase, and cysteine synthaseshowed a significant decline beyond 100 μM. The level of proline, glycine betaine and total phenolics also showed decreasing trend with the increase in Cu concentration. In conclusion, plantsare potential phytoextractor of Cu but do suffer from the toxic effects of Cu at high concentration of 500 μM. Sesuvium plants therefore appear suitable for use in phytoremediation purpose at low Cu concentrations (100 – 250 μM).

  • ft ir profiling reveals differential response of roots and leaves to salt stress in a halophyte Sesuvium portulacastrum l l
    Biotechnology Reports, 2019
    Co-Authors: T. D. Nikam, Ganesh C. Nikalje, Jitendra Kumar, Penna Suprasanna
    Abstract:

    Abstract In a halophyte, Sesuvium portulacastrum (L.) L., we have applied Fourier Transform InfraRed (FT-IR) spectroscopy to detect the corresponding changes associated with salt-induced physiological changes under long- term treatment with 0, 100 and 500 mM NaCl. FT-IR profiles showed changes in chemical composition and functional groups of proteins, lipids and carbohydrates due to salt treatments, evident as differential FT-IR profiles in both roots and leaves specific to these metabolites. Further, the Principle Component Analysis (PCA) was applied to identify the main sources of variation in FT-IR data due to differential treatment. In PCA, the PC1 showed 85.55% and PC2 showed 18.18% variability in data and confirmed differential response of root and leaves to salt treatment in Sesuvium. The results suggest that FT-IR spectrometry can be used to study stress-induced metabolic changes in plants in relation to their salt tolerance.

  • identification and validation of reference genes for quantitative real time pcr under salt stress in a halophyte Sesuvium portulacastrum
    Plant Gene, 2018
    Co-Authors: Ganesh C. Nikalje, T. D. Nikam, Ashish Kumar Srivastava, Gaurav Sablok, Girdhar K Pandey, Penna Suprasanna
    Abstract:

    Abstract Sesuvium portulacastrum L. is a facultative halophyte with potential applications in phytoremediation and phytodesalination and offers as a system to understand mechanisms of salt adaptation. However, studies on gene identification, characterization and transcriptomics of this halophyte are limited due the paucity of genome sequence information and validation of gene expression data through quantitative real time-PCR (qRT-PCR). qRT-PCR is a reliable method for exact quantification of gene expression, but is dependent upon the selection of suitable reference gene(s). In the present study, using Sesuvium RNA-Seq data, we identified ten reference genes such as elongation factor 1-α, ubiquitin-conjugating enzyme E2, TATA-box binding protein, DnaJ-like protein, β-tubulin, glyceraldehyde 3-phosphate dehydrogenase, eukaryotic initiation factor 4a, RNA-dependent RNA polymerase 1, α-tubulin and pentatricopeptide repeat and validated their stable expression in root and shoot tissues under 0, 100 and 250 mM NaCl stress conditions. Five different statistical methods viz. Delta Ct, NormFinder, BestKeeper, geNorm and ReFinder were used to establish the most suitable reference genes for root (UCE 2, TBP, EF1-α) and shoot (α-TUB, EIF4a, EF1-α). Additionally, the expression of five salt responsive genes was calculated under salt stress using the best reference genes and compared with RNA-Seq data. The comparison between real time and RNA-Seq data showed a high correlation (r2 = 0.875), supporting the selection of the suitable reference genes. This study provides a good starting platform for successful gene quantification in S. portualcastrum.

  • Temporal and spatial changes in ion homeostasis, antioxidant defense and accumulation of flavonoids and glycolipid in a halophyte Sesuvium portulacastrum (L.) L. - Fig 10
    2018
    Co-Authors: Ganesh C. Nikalje, P. S. Variyar, M. V. Joshi, T. D. Nikam, Penna Suprasanna
    Abstract:

    Time dependant accumulation of C) potasssium in roots D) potassium in leaves at 2hr, 6hr, 24 hr, 3d, 5d and 7d in Sesuvium portulacastrum under 0, 150 and 500 mM NaCl treatment. All values are means of three independent biological replicates. The significance of mean difference (P

Vinayak H. Lokhande - One of the best experts on this subject based on the ideXlab platform.

  • copper accumulation and biochemical responses of Sesuvium portulacastrum l
    Materials Today: Proceedings, 2020
    Co-Authors: Vinayak H. Lokhande, Penna Suprasanna, Vikas Yadav Patade, Sudhakar Srivastava, Manoj Shrivastava, Garima Awasthi
    Abstract:

    Abstract Sesuvium portulacastrum (L.) is a halophytic phytoremediator plant with potential for significant accumulation of metals and metalloids. The present work evaluatedthe physiological and biochemical responses of Sesuvium plants to copper (Cu) exposure (100–500 μM) for 30 d in field conditions. Plants demonstrated significant copper accumulation that increased with the increase in Cu concentration of the medium (maximum 254 µg g−1 DW at 500 µM). The root dry weight was not significantly affected at 500 μM while shoot dry weight decreased significantly. Total soluble proteins, photosynthetic pigmentsand malondialdehye (MDA) were declined significantly beyond 100 μM after 30 d. Among metabolites and enzymes of thiol metabolism, total non-protein thiols (NP-SH), γ-glutamylcysteine synthetase and glutathione reductase did not show significant effect while cysteine, serine acetyltransferase, and cysteine synthaseshowed a significant decline beyond 100 μM. The level of proline, glycine betaine and total phenolics also showed decreasing trend with the increase in Cu concentration. In conclusion, plantsare potential phytoextractor of Cu but do suffer from the toxic effects of Cu at high concentration of 500 μM. Sesuvium plants therefore appear suitable for use in phytoremediation purpose at low Cu concentrations (100 – 250 μM).

  • salt stress reveals differential antioxidant and energetics responses in glycophyte brassica juncea l and halophyte Sesuvium portulacastrum l
    Frontiers in Environmental Science, 2015
    Co-Authors: Ashish Kumar Srivastava, Vinayak H. Lokhande, Sudhakar Srivastava, S F Dsouza, Penna Suprasanna
    Abstract:

    Salt stress, considered as one of the major factors, decreases crop productivity world-wide and hence, investigations are being made to understand the cellular basis of salt tolerance in plants. In our earlier studies, maintenance of redox homeostasis and energetics were found as key determinants of salt tolerance in a halophyte Sesuvium portulacastrum (high salt accumulator). The redox homeostasis is defined as integrated ratio of different redox couples present inside the cell. In recent years, it has also been proposed as general stress response regulator in plants, bacteria as well as animals. In view of this, present study was performed to compare responses of redox state and energetics of S. portulacastrum with a glycophyte Brassica juncea (low salt accumulator). The data revealed activation of antioxidant defense in S. portulacastrum which either avoided or delayed the accumulation of different reactive oxygen species (ROS). In contrast, due to the lack of co-ordination, although the non-enzymatic antioxidants were increased, significant oxidative damage was seen in B. juncea. Further, the decreased NADPH oxidase activity suggested that basal redox signaling was also affected in B. juncea. In order to correlate these changes with chloroplastic and mitochondrial electron transport chain, NADP/NADPH and NAD/NADH ratios were measured. The NADP/NADPH ratio suggested that the process of photosynthesis was minimally affected in S. portulacastrum which might have contributed to its lower level of ROS under salt stress. The comparatively lower NAD/NADH and ATP/ADP ratios in S. portulacastrum as compared to B. juncea indicated the active and better utilization of energy generated to support different processes associated with salt tolerance. Thus, the findings suggest that co-ordinated regulation of antioxidant defense to avoid oxidative damage and proper utilization of energy are the key determinants of salt-tolerance in plants.

  • Hairy root induction and phytoremediation of textile dye, Reactive green 19A-HE4BD, in a halophyte, Sesuvium portulacastrum (L.) L.
    Biotechnology Reports, 2015
    Co-Authors: Vinayak H. Lokhande, Subhash Kudale, Ganesh Nikalje, Neetin Desai, Penna Suprasanna
    Abstract:

    In this study, we report phytoremediation of textile dyes using hairy roots derived through Agrobacterium rhizogenes (NCIM 5140) infection of in vitro leaf and stem explants of a halophyte Sesuvium portulacastrum (L.) L. Leaf explants showed higher frequency of hairy root induction (70%) than stem explants (30%), and maximum number of roots (leaf 42.3 ± 2.4 and stem 50.3 ± 1.7). Transformed nature of hairy roots was ascertained by amplifying 970 bp region of T-DNA of Ri plasmid. Hairy roots were screened for phytoremediation of various textile dyes and results showed that HRs were able to degrade Reactive green 19A HE4BD upto 98% within 5 days of incubation. Spectrophotometric analysis showed decrease in dye concentration while HPLC and FTIR analysis confirmed its degradation. Seed germination assay demonstrated non-toxic nature of the extracted metabolites. This is the first report on induction of hairy root culture in Sesuvium portulacastrum and phytoremediation of textile dyes.

  • biochemical and physiological adaptations of the halophyte Sesuvium portulacastrum l l aizoaceae to salinity
    Archives of Agronomy and Soil Science, 2013
    Co-Authors: Vinayak H. Lokhande, T. D. Nikam, Ketki V Mulye, Rohini Patkar, Penna Suprasanna
    Abstract:

    Sesuvium portulacastrum is a dicotyledonous halophyte. The responses of different clones of Sesuvium to salinity were analysed by measuring changes in growth, biomass accumulation, water content, osmolytes accumulation, oxidative damage, antioxidant enzymes and inorganic ions. In addition, microscopic observations were made to discern any changes in the stem anatomy of Sesuvium under salt stress. Reduced growth, biomass accumulation and tissue water content correlated with an increase in NaCl concentrations (200–800 mM), except at 200 mM NaCl, where an improvement in the parameters was observed among all clones, specifically in MH1 (Maharashtra). Increased osmolytes (proline, glycine betaine and total soluble sugars) and the accumulation of Na+ ions, without affecting K+content, were recorded in all clones. Higher malondialdehyde content and greater relative electrolyte leakage were evident in addition to increased catalase and superoxide dismutase activities under salt stress. Increased cortical cell siz...

  • Sesuvium portulacastrum l l a potential halophyte for the degradation of toxic textile dye green he4b
    Planta, 2012
    Co-Authors: Asmita V Patil, Vinayak H. Lokhande, Penna Suprasanna, V A Bapat, Jyoti P Jadhav
    Abstract:

    Sesuvium portulacastrum is a common halophyte growing well in adverse surroundings and is exploited mainly for the environmental protection including phytoremediation, desalination and stabilization of contaminated soil. In the present investigation, attempts have been made on the decolorization of a toxic textile dye Green HE4B (GHE4B) using in vitro grown Sesuvium plantlets. The plantlets exhibited significant (70%) decolorization of GHE4B (50 mg l−1) that sustain 200 mM sodium chloride (NaCl) within 5 days of incubation. The enzymatic analysis performed on the root and shoot tissues of the in vitro plantlets subjected to GHE4B decolorization in the presence of 200 mM NaCl showed a noteworthy induction of tyrosinase, lignin peroxidase and NADH-DCIP reductase activities, indicating the involvement of these enzymes in the metabolism of the dye GHE4B. The UV–visible spectrophotometer, HPLC and Fourier Transform Infrared Spectroscopy (FTIR) analyses of the samples before and after decolorization of the dye confirmed the efficient phytotransformation of GHE4B in the presence of 200 mM NaCl. Gas Chromatography–Mass Spectroscopy (GC–MS) analysis of the products revealed the formation of three metabolites such as p -amino benzene, p -amino toluene and 1, 2, 7-amino naphthalene after phytotransformation of GHE4B. Based on the FTIR and GC–MS results, the possible pathway for the biodegradation of GHE4B in the presence of 200 mM NaCl has been proposed. The phytotoxicity experiments confirmed the non-toxicity of the degraded products. The present study demonstrates for the first time the potential of Sesuvium for the efficient degradation of textile dyes and its efficacy on saline soils contaminated with toxic compounds.

Inès Slama - One of the best experts on this subject based on the ideXlab platform.

  • effects of salt treatment on growth lipid membrane peroxidation polyphenol content and antioxidant activities in leaves of Sesuvium portulacastrum l
    Arid Land Research and Management, 2017
    Co-Authors: Inès Slama, Rawya Mrabet, Riadh Ksouri, Ons Talbi, Ahmed Debez, Chedly Abdelly
    Abstract:

    ABSTRACTChanges in growth, leaf contents of proline, oxidative stress-related parameters, and phenolic compounds, and antioxidant activities were investigated in the halophyte species Sesuvium portulacastrum L. under saline conditions. Rooted cuttings were individually cultivated in sandy soil. After five weeks of pre-treatment, seedlings were submitted during one month to different salt concentrations ranging from 0 to 800 mM NaCl. The plant growth was significantly improved by salt at 200–600 mM concentration. This trend was associated with (i) the stimulation of photosynthetic activity, (ii) the protection of membrane integrity (leaf MDA content 50% lower than the control), and (iii) higher total antioxidant activity, especially at 400 mM NaCl. At this salt concentration plants accumulated high contents of proline, polyphenols, antocyanins, and carotenoids. These compounds could be implied in the protection of the photosynthetic system and in the improvement of growth. Exposure to 800 mM NaCl impaired ...

  • water deficit stress applied only or combined with salinity affects physiological parameters and antioxidant capacity in Sesuvium portulacastrum
    Flora, 2015
    Co-Authors: Inès Slama, Rawya Mrabet, Riadh Ksouri, Ons Talbi, Ahmed Debez, Chedly Abdelly
    Abstract:

    Abstract Sesuvium portulacastrum is a promising halophyte well adapted to salt and to drought. However, no information in the literature was available about its antioxidant capacity. The objective of this study is to investigate the effects of water deficit applied alone or combined with salinity on some physiological parameters, proline and polyphenol accumulation, and antioxidant and antiradical activities. Seedlings were cultivated under optimal or limiting water supply (respectively, 100% and 25% of field capacity, FC). The amount of the evapotranspirated water was replaced by a nutrient solution containing either 0 or 200 mM NaCl. Water deficit stress reduced plant growth together with a significant decline in leaf water content. Salinity mitigated the deleterious effects of water deficit stress on growth and led to an increase in net CO2 assimilation and stomatal conductance. In addition, salt supply under water deficit significantly increased leaf proline concentration and reduced lipid membrane peroxidation, assessed by leaf malondialdehyde concentration. Phenolic compound content increased significantly when plants were transferred from water deficit stress to 100% FC. However, plants subjected to water deficit stress combined to salt had high polyphenol content and the highest level of the antiradical activity in their stems. As a whole, cultivating S. portulacastrum under water deficit stress combined with salinity might be an interesting approach to exploit antioxidant metabolites from this species for medicinal and industrial purposes. Recovery of most of the studied parameters was substantial following relief of stress. Drought stress alone or combined with salinity did not cause permanent alterations in S. portulacastrum plants, which conserve their growth potentialities, and which can be a useful species in re-vegetation programs in arid saline areas.

  • combined effects of long term salinity and soil drying on growth water relations nutrient status and proline accumulation of Sesuvium portulacastrum
    Comptes Rendus Biologies, 2008
    Co-Authors: Inès Slama, Tahar Ghnaya, Arnould Savoure, Chedly Abdelly
    Abstract:

    Abstract The interaction between soil drying and salinity was studied in the perennial halophyte, Sesuvium portulacastrum. Rooted cuttings were individually cultivated for three months in silty–sandy soil under two irrigation modes: 100 and 25% of field capacity (FC). The amount of the evapotranspirated water was replaced by a nutrient solution containing either 0 or 100 mM NaCl. Whole-plant growth, leaf water content, leaf water potential ( Ψ w ), and Na+, K+, and proline concentrations in the tissues were measured. When individually applied, both drought and salinity significantly restricted whole-plant growth, with a more marked effect of the former stress. However, the effects of the two stresses were not additive on whole-plant biomass or on leaf expansion. Root growth was more sensitive to salt than to soil drying, the latter being even magnified by the adverse impact of salinity. Leaf water content was significantly reduced following exposure to water-deficit stress, but was less affected in salt-treated plants. When simultaneously submitted to water-deficit stress and salinity, plants displayed higher values of water and potassium use efficiencies, leaf proline and Na+ concentrations, associated with lower leaf water potential (−1.87 MPa), suggesting the ability of S. portulacastrum to use Na+ and proline for osmotic adjustment. To cite this article: I. Slama et al., C. R. Biologies 331 (2008).

  • comparative study of the effects of mannitol and peg osmotic stress on growth and solute accumulation in Sesuvium portulacastrum
    Environmental and Experimental Botany, 2007
    Co-Authors: Inès Slama, Tahar Ghnaya, Dorsaf Messedi, Arnould Savoure, Kamel Hessini, Chedly Abdelly
    Abstract:

    Abstract Responses to osmotic stress were studied in Sesuvium portulacastrum , a halophyte potentially usable for saline soil stabilization and covering. Cuttings were multiplicated and cultivated in nutrient solution supplemented with 100 mM NaCl. They were exposed for 12 days to osmotic stress induced by either mannitol or polyethylene glycol (PEG 6000). Growth, tissue water content, relative water content, and contents in inorganic (Na + , K + ) and organic (proline, soluble sugars) solutes were determined at regular intervals. Both PEG and mannitol reduced growth , decreased leaf number and leaf mean surface area, and led to a significant reduction of leaf water and K + contents. However, these effects were significantly less severe in plants submitted to mannitol, as compared to PEG-treated ones. The contents in soluble sugars contents and in Na + remained unchanged, while that of proline strongly increased, particularly in mannitol-stressed plants. A positive relationship was observed between growth, relative water content, potassium content, and proline accumulation in leaves. However, proline remained a minor component in the pool of solutes.

  • cd induced growth reduction in the halophyte Sesuvium portulacastrum is significantly improved by nacl
    Journal of Plant Research, 2007
    Co-Authors: Tahar Ghnaya, Inès Slama, Dorsaf Messedi, Claude Grignon, Mohamed Habib Ghorbel, Chedly Abdelly
    Abstract:

    The effects of Cd2+ and NaCl, applied together or separately, on growth and uptake of Cd2+ were determined for the halophyte Sesuvium portulacastrum L. Seedlings were cultivated in the presence of 50 or 100 μmol L−1 Cd2+ alone or combined with 100 or 400 mmol L−1 NaCl. Data showed that alone, Cd2+ induced chlorosis, necrosis, and inhibited growth. Addition of NaCl to Cd2+-containing medium restored growth and alleviated the toxicity, however. NaCl also enhanced the amounts of Cd2+ accumulated in the shoots. All Cd2+ treatment reduced K+ and Ca2+ uptake and transport to the shoots. Accumulation of Na+ in the shoots was not affected by Cd2+, however. Thus S. portulacastrum maintained its halophytic characteristics in the presence of Cd2+. We suggest this halophyte could be used for phytoextraction of Cd2+ from salt-contaminated sites.