The Experts below are selected from a list of 432 Experts worldwide ranked by ideXlab platform
Norma Maria Barros Benevides - One of the best experts on this subject based on the ideXlab platform.
-
involvement of the dopaminergic system in the antidepressant like effect of the lectin isolated from the red marine alga Solieria filiformis in mice
International Journal of Biological Macromolecules, 2017Co-Authors: Ticiana Monteiro Abreu, Felipe Barros Teles, Erika Freitas Mota, Valdecio Silvano Monteiro, Ana Beatriz Souza Martins, Renata Line Da Conceicao Rivanor, Danielle Silveira Macedo, Silvânia Maria Mendes Vasconcelos, Jose Eduardo Ribeiro Honorio, Norma Maria Barros BenevidesAbstract:This study aimed at evaluating the antidepressant-like action of the marine alga Solieria filiformis lectin (SfL) and to investigate the participation of the monoaminergic system in this action. For this, male Swiss mice (n=10) were pretreated with intravenous injections (i.v.) of SfL (1, 3 or 9mg/kg) and submitted to open field (OFT), tail suspension (TST), forced swimming (FST), elevated plus-maze (EPMT) and hole-board tests (HBT). As controls, mice received sterile saline (i.v.), imipramine (10 or 30mg/kg; intraperitoneally - i.p.) or diazepam (1 mk/kg; i.p.). To assess the involvement of the monoaminergic system in SfL effects, the FST was conducted in mice pretreated with PCPA, an inhibitor of serotonin synthesis, or noradrenergic and dopaminergic receptors specific antagonists. The results showed that SfL has an antidepressant-like effect, with no psychostimulant and anxiolytic-like effects. When denatured or combined with mannan, SfL lost the ability to reduce the immobility time in the FST. In addition, SfL antidepressant-like effect was inhibited by the pretreatment of mice with SCH 23390, a dopamine D1 receptor antagonist, and by sulpiride, a dopamine D2 receptor antagonist. Thus, SfL produced an antidepressant-like effect, which is probably dependent on its interaction with the dopaminergic system.
-
optimized acid hydrolysis of the polysaccharides from the seaweed Solieria filiformis kutzing p w gabrielson for bioethanol production
Acta Scientiarum. Biological Sciences, 2017Co-Authors: George Meredite Cunha De Castro, Norma Maria Barros Benevides, Maulori Curie Cabral, Rafael De Souza Miranda, Eneas Gomes Filho, Maria Valderez Ponte Rocha, Marjory Lima Holanda AraujoAbstract:The seaweeds are bio-resource rich in sulfated and neutral polysaccharides. The tropical seaweed species used in this study ( Solieria filiformis ), after dried, shows 65.8% (w/w) carbohydrate, 9.6% (w/w) protein, 1.7% (w/w) lipid, 7.0% (w/w) moisture and 15.9% (w/w) ash. The dried seaweed was easily hydrolyzed under mild conditions (0.5 M sulfuric acid, 20 min.), generating fermentable monosaccharides with a maximum hydrolysis efficiency of 63.21%. Galactose and glucose present in the hydrolyzed were simultaneously fermented by Saccharomyces cerevisiae when the yeast was acclimated to galactose and cultivated in broth containing only galactose. The kinetic parameters of the fermentation of the seaweed hydrolyzed were Y (P ⁄ S) = 0.48 ± 0.02 g.g −1 , P P = 0.27 ± 0.04 g.L −1 .h −1 , h = 94.1%, representing a 41% increase in bioethanol productivity. Therefore, S. filiformis was a promising renewable resource of polysaccharides easily hydrolyzed, generating a broth rich in fermentable monosaccharides for ethanol production.
-
purificacao do pigmento r ficoeritrina da macroalga marinha Solieria filiformis
Encontros Universitários da UFC, 2017Co-Authors: Aurea Anette Monteiro Brito, Norma Maria Barros Benevides, Acrisio Jose Uchoa Bastosfilho, Roberta Costa, Antonio Augusto Lima Araujofilho, Marjory Lima Holanda AraujoAbstract:As macroalgas marinhas, por biossintetizarem compostos fenolicos, polissacarideos, proteinas, peptideos e pigmentos, tem sido utilizadas como alimento e como fontes de compostos bioativos em diferentes areas industriais. Dentre esses compostos bioativos destacam-se os pigmentos fotossinteticos das macroalgas vermelhas, como as ficoeritrinas, os quais sao moleculas que apresentam alta fluorescencia e coloracao vermelha caracteristica. Sao moleculas importantes para industria diante da carencia de pigmentos naturais e fluorescentes. Nesse contexto, o objetivo desse trabalho foi purificar o pigmento fluorescente R-ficoeritrina da macroalga marinha Solieria filiformis, abundante no litoral nordestino brasileiro e com alto potencial para cultivo no mar. Um esquema de purificacao com precipitacao proteica (sulfato de amonio 90% de saturacao), seguido por cromatografias em matrizes de DEAE-Sephacel e Sephacryl S-300, gerou um pigmento purificado apresentando tres picos de absorcao (495, 540 e 564 nm), um pico de emissao de fluorescencia (575 nm, quando excitada a 495 nm), e um indice de pureza de 4,5, possibilitando sua aplicacao como marcador fluorescente na biotecnologia. O processo de purificacao da R-FE apresentou uma recuperacao de 12,78% e um rendimento de 0,17 mg/g de alga seca. Por eletroforese (PAGE-SDS) a R-FE apresentou uma banda proteica na ausencia do agente redutor. Diante dos resultados, foi estabelecido um protocolo de purificacao para R-ficoeritrina de S. filiformis, apresentando um elevado indice de pureza podendo ser aplicado em metodos biotecnologicos.
-
otimizacao da hidrolise acida da macroalga vermelha Solieria filiformis visando a geracao de alto teor de galactose
Encontros Universitários da UFC, 2017Co-Authors: Caroline Landim Peixoto, Norma Maria Barros Benevides, George Meredite Cunha De Castro, Maria Valderez Ponte Rocha, Antonio Alves Da Silva Neto, Marjory Lima Holanda AraujoAbstract:As algas marinhas tem despertado o interesse de pesquisadores devido seu alto teor de polissacarideos, principalmente como fonte renovavel de biomassa para a obtencao de carboidratos fermentesciveis. A alga marinha Solieria filiformis (Kutzing) P. W. Gabrielson (Rhodophyta, Gigartinales, Solieriaceae) e uma especie abundante no litoral brasileiro e com potencial para ser cultivada em sistema de maricultura no litoral do nordeste do Brasil. O presente trabalho teve como objetivo otimizar a hidrolise da alga vermelha S. filiformis utilizando a Metodologia de Superficie de Resposta (MSR) a fim de obter um meio liquido com altos teores de galactose. O delineamento experimental foi elaborado com o auxilio do software Statistica, com o total de 17 experimentos independentes de hidrolise, para o estudo da geracao de Galactose com tres parâmetros considerados (concentracao de acido sulfurico, quantidade de biomassa e tempo de reacao). A alga vermelha S. filiformis foi coletada em estruturas de cultivo na praia de Flecheiras, Trairi (CE), lavada, seca a 25°C, triturada e padronizada em 20 mesh. As hidrolises foram realizadas a 120 °C em autoclave, com concentracao de acido, quantidade de alga e tempo de processo de cada experimento determinados pelo delineamento elaborado. Os resultados obtidos foram analisados pelo teste de variância (ANOVA) e a influencia de cada parâmetro sobre a resposta foi estudada. A partir desses dados, foram gerados um modelo matematico, uma superficie de resposta e uma curva de contorno. A otimizacao gerou resultados in silico de 154,74 g.L-1 de galactose para a utilizacao dos parâmetros de 1,8 M de acido sulfurico, 665,7 g.L-1 de biomassa e 32,9 min de processo. Com esses resultados, concluimos que foi possivel otimizar a hidrolise acida de S. filiformis para producao de altos teores de galactose, com a geracao de modelos matematicos preditivos com confiabilidade estatistica para a resposta estudada.
-
in vitro inhibition of thrombin generation by sulfated polysaccharides from the marine alga Solieria filiformis kutzing gabrielson Solieriaceae rhodophyta inibicao in vitro de geracao de trombina por polissacarideos sulfatados da alga marinha solieri
Acta of Fisheries and Aquatic Resources, 2017Co-Authors: Renato Martins Andrade, Jose Arievilo Gurgel Rodrigues, Ianna Vivianne Fernandes De Araujo, Norma Maria Barros Benevides, Ana M F Tovar, Paulo A S MouraoAbstract:Abstract Solieria filiformis is a rich source in carrageenans-type sulfated polysaccharides (SPs) containing two fractions (F I and F II), of which F I presented in vivo antinociceptive and inflammatory actions. However, the effects of its SPs on thrombin generation (TG) assays have not been investigated. This study characterized the physicochemical properties of SPs isolated from Solieria filiformis cultured in the coast of Ceara, Brazil and analyzed in vitro inhibitory effects on a TG system using a chromogenic method by continuous detection. Crude extract obtained with papain and fractionated by anion-exchange chromatography on a DEAE-cellulose column contained two fractions (F I and F II). Electrophoresis in agarose gel revealed an difference in terms of charge density between extract and fractions obtained from the DEAE-cellulose chromatography, suggesting the presence of both kappa- and iota-carrageenans, confirming a previous study using the respective S. filiformis fractions. Anticoagulant assays using normal human plasma and unfractionated heparin (UHEP) (193 IU mg-1) by activated partial thromboplastin time test showed virtual effects in order of 1.34 (extract), 0.86 (F I) and 0.98 (F II) IU mg-1 less potents than UHEP; additionally, extract and fractions had no actions on the prothrombin time. 60-fold diluted human plasma treated with extract and fractions inhibited dependently of concentration TG stimulated by cephalin (intrinsic pathway) and thromboplastin (extrinsic pathway), with charge density-related TG inhibition pattern of the algal SPs. UHEP totally abolished TG at 4.15-fold lower concentration. Therefore, SPs from S. filiformis prevent thrombosis, in vitro, by interfering with the TG system, as a promising plasma alternative to modulate blood coagulation.Resumo Solieria filiformis e uma fonte rica em polissacarideos sulfatados (PSs) tipo carragenanas contendo duas fracoes (F I e F II), das quais F I apresentou acoes antinociceptiva e inflamatoria in vivo. Entretanto, nao tem sido investigados os efeitos de seus PSs sobre ensaios de geracao de trombina (GT). Este estudo caracterizou as propriedades fisico-quimicas de PSs isolados de Solieria filiformis cultivada na costa do Ceara, Brasil e analisada efeitos inibitorios in vitro sobre um sistema de GT usando um metodo cromogenico por deteccao continua. O extrato bruto obtido com papaina e fracionado por cromatografia de troca-ionica em uma coluna de DEAE-celulose conteve duas fracoes (F I e F II). Eletrophorese em gel de agarose revelou diferenca de densidade de cargas entre extrato e fracoes obtidas da cromatografia em DEAE-celulose, sugerindo a presenca de ambas carragenanas-kappa e -iota, respectivamente, e confirmando, ainda, um estudo previo usando as fracoes de S. filiformis. Ensaios anticoagulantes usando plasma humano e heparina nao-fracionada (HEPNF) (193 UI mg-1) pelo teste do tempo de tromboplastina parcial ativada mostraram efeitos virtuais na ordem de 1,34 (extrato), 0,86 (F I) e 0,98 (F II) UI mg-1 potentes menos que HEPNF. Ademais, extrato e fracoes nao possuiram acoes sobre o time de protrombina. Plasma humano diluido 60 vezes tratado com extrato e fracoes inibiu GT estimulada por cefalina (via intrinsica) e tromboplastina (via extrinsica), com grau de inibicao relacionada a densidade de carga dos PSs da alga. HEPNF aboliu totalmente GT em concentracao 4,15 vezes menor. Os PSs de S. filiformis previnem trombose in vitro por interferir com o sistema de GT, alternativa promissora no plasma para modular a coagulacao sanguinea.
Gilles Bedoux - One of the best experts on this subject based on the ideXlab platform.
-
Environmentally Friendly Valorization of Solieria filiformis (Gigartinales, Rhodophyta) from IMTA Using a Biorefinery Concept
Marine drugs, 2018Co-Authors: Ana Peñuela, Gilles Bedoux, Nathalie Bourgougnon, Daniel Robledo, Emanuel Hernández-núñez, Yolanda Freile-pelegrínAbstract:Marine macroalgae (seaweed) are an excellent source of novel bioactive metabolites. The biorefinery concept applied to seaweed facilitates the extraction of many chemical constituents from the same biomass ensuring that the resource is used fully, generating few residues through a succession of extraction steps. In the present study, the biomass of the carragenophyte Solieria filiformis (Rhodophyta, Gigartinales) cultured in an integrated multi-trophic aquaculture (IMTA) system was evaluated to obtain valuable products by a biorefinery approach. Enzymatic-assisted extraction (EAE) and microwave-assisted extraction (MAE) were the eco-friendly technologies used to ensure an environmentally friendly valorization of the biomass. Three valuable products were successfully recovered: a water-soluble extract rich in proteins and sulfated polysaccharides suitable as a food supplement; a lipid fraction rich in polyunsaturated fatty acids (PUFAs) with potential to be used in the nutraceutical industry; and a pure ι-carrageenan with a powerful antiviral activity against Herpes simplex virus (EC 50 = 6.3 µg mL −1) comparable to the commercial antiviral acyclovir (EC 50 = 3.2-5.4 µg mL −1).
-
Radical scavenging activity of lipids from seaweeds isolated by solid-liquid extraction and supercritical fluids
Oléagineux Corps Gras Lipides, 2018Co-Authors: Nolwenn Terme, Romain Boulho, Jean-philippe Kucma, Nathalie Bourgougnon, Gilles BedouxAbstract:In vitro antioxidant activities of the lipid fractions from two selected seaweeds, Solieria chordalis and Sargassum muticum were investigated according to the extraction methods. The activity of neutral lipids, glycolipids and phospholipids, thanks to extraction by chloroform/methanol (1/1 v/v; CM) mixture, pure supercritical carbon dioxide (sc-CO2), supercritical carbon dioxide with 2% of ethanol (sc-CO2 + EtOH 2%) and supercritical carbon dioxide with 8% of ethanol as co-solvent (sc-CO2 + EtOH 8%), were studied using DPPH radical scavenging assays. All the lipid classes demonstrated a free radical scavenging activity at the concentration of 1 mg/ml. The best scavenging activity (86.6 +/- 5.7%) was obtained when the neutral lipid fraction was extracted from S. chordalis with a CM mixture. The neutral lipid fraction extracted with sc-CO2 showed a lower activity than those obtained with solvents. However, the addition of ethanol in sc-CO(2 )did not affect the antioxidant activity of neutral lipids fixed at around 16% of radical scavenging. For S. muticum, the activity of glycolipids (50.9 +/- 0.8%) and phospholipids (48.4 +/- 1.6%) obtained with sc-CO2 were twice as large as that of fractions obtained with CM, 29.6 +/- 3.4% and 28.0 +/- 4.2%, respectively. The activity of neutral lipids did not change with the extraction method with around 25% of radical scavenging. This is the first report of free radical scavenging activity of lipid classes obtained by supercritical carbon dioxide extraction from seaweeds.
-
Selective extraction of lipid classes from Solieria chordalis and Sargassum muticum using supercritical carbon dioxide and conventional solid–liquid methods
Journal of Applied Phycology, 2017Co-Authors: Nolwenn Terme, Romain Boulho, Melha Kendel, Jean-philippe Kucma, Gaëtane Wielgosz-collin, Nathalie Bourgougnon, Gilles BedouxAbstract:Lipid extraction yields and lipid class distribution from two seaweeds Solieria chordalis (Rhodophyceae) and Sargassum muticum (Phaeophyceae) were investigated using different extraction methods. Supercritical carbon dioxide extraction with or without co-solvent and chloroform/methanol extractions were applied to S. chordalis and S. muticum. Total lipid contents ranged between 0.13 and 2.96% dry weight (dw) and between 0.8 and 3.15% dw for S. chordalis and S. muticum, respectively. The best yields were obtained from chloroform/methanol mixture. A promising 1.21% dw yield was obtained from supercritical CO2 with 2% ethanol for S. chordalis. The proportion of the lipid classes drastically changed according to the method. By using the chloroform/methanol Bligh and Dyer mixture, the main fractions extracted from S. chordalis were neutral lipids (37%) and glycolipids (38%) whereas glycolipids (60%) were mainly isolated using pure supercritical CO2. An addition of ethanol (2%) as co-solvent enhanced the neutral lipid fraction up to 56%. The glycolipid fraction reached 82.2% of total lipids when S. muticum was treated by supercritical CO2, and 61.2 to 70.8% for chloroform/methanol procedures. More broadly, polar lipid ratio (glycolipids and phospholipids) was important in the brown seaweed with 86 to 95% of total lipid content. These results highlighted the relationships between the extraction process and the lipid class distribution.
-
antiherpetic hsv 1 activity of carrageenans from the red seaweed Solieria chordalis rhodophyta gigartinales extracted by microwave assisted extraction mae
Journal of Applied Phycology, 2017Co-Authors: Romain Boulho, Nathalie Bourgougnon, Daniel Robledo, Yolanda Freilepelegrin, Christel Marty, Gilles BedouxAbstract:Carrageenan yield, physicochemical properties, and antiviral activity of the carrageenan from Solieria chordalis (Rhodophyceae, Solieriaceae) harvested at the Brittany coast (France) were investigated. S. chordalis carrageenan was extracted by conventional and the microwave-assisted extraction (MAE) methods. The effect of different parameters during MAE extraction such as alkali concentration (0, 0.5 and 1% KOH), extraction time (10, 20, and 25 min) and temperature (90 and 105 °C) were evaluated. Native carrageenan extracted by MAE had the highest yield (29.3%) after 10 min at 90 °C. After alkali treatment, carrageenan yield ranged from 10.7 to 18.4%. No significant differences in the carrageenan yield were observed between MAE and conventional method under alkaline conditions. Chemical analysis and FTIR spectra revealed the presence of a predominant iota-carrageenan. Evaluation of the antiviral activity of S. chordalis carrageenan against HSV-1 (Herpes simplex virus type 1) showed a EC50 of the iota-carrageenans fractions in the range of 3.2 to 54.4 μg mL−1 (MOI 0.01 ID50 mL−1) without cytotoxicity in that range of concentrations.
-
fractionation of uv b absorbing molecules and of free radical scavenging compounds from Solieria chordalis by using centrifugal partition chromatography
Phytochemistry Letters, 2017Co-Authors: Romain Boulho, Nathalie Bourgougnon, Le J Roux, Le C Quemener, Gregoire Audo, Gilles BedouxAbstract:Abstract Liquid-liquid chromatography can lead to the isolation of compounds or enrichment of fractions. To investigate the structural characteristics of antioxidants and UV-B absorbing molecules, a preparative centrifugal partition chromatography (CPC) method was developed to prepare enriched bioactive fractions from the red seaweed Solieria chordalis (Gigartinales, Solieriaceae). After a methanolic extraction of dried Solieria chordalis and liquid-liquid separation using ethyl acetate/water (v/v), the ethyl acetate phase was submitted to CPC using Arizona Y of the quaternary solvent system composed of n-heptane/ethyl acetate/methanol/water (19/1/19/1, v/v) in ascending mode. Among the fifteen collected fractions, three fractions gave up to 23.50% of DPPH radical scavenging activity. The CPC fractionation was monitored by HPLC and, four compounds exhibited UV-B absorbing capacity regarding their absorption at 310 nm.
Jeanyves Floch - One of the best experts on this subject based on the ideXlab platform.
-
partial purification and characterization of udp glucose 4 epimerase from Solieria chordalis rhodophyceae
Botanica Marina, 2003Co-Authors: Fabienne Goulard, Eric Deslandes, P Pondaven, M Diouris, Jeanyves FlochAbstract:The partial purification of UDP-glucose-4-epimerase was carried out on the red macroalga Solieria chordalis. A 60-fold purification factor was obtained by chromatography on DEAE Protein-Pack 8 HR and Superose 12. Gel-filtration fast protein liquid chromatography (FPLC) showed that the enzyme had an apparent molecular mass of 130 kDa. The UDP-glucose-4-epimerase from Solieria chordalis does not require complementary NAD addition for activity, unlike the enzyme from some other organisms. Moreover, the optimal pH was at 8.5 and the apparent K M values for UDP-glucose and UDP-galactose were 87 μM and 630 μM, respectively.
-
an hplc method for the assay of udp glucose pyrophosphorylase and udp glucose 4 epimerase in Solieria chordalis rhodophyceae
Phytochemical Analysis, 2001Co-Authors: Fabienne Goulard, M Diouris, E Deslandes, Jeanyves FlochAbstract:An efficient method to assay both UDP-glucose pyrophosphorylase and UDP-glucose-4-epimerase in a crude extract of the red seaweed, Solieria chordalis is described. The method is based on the direct quantification by reverse-phase high-performance liquid chromatography of the UDP-sugars generated in the reaction mixture. UDP-glucose, UDP-galactose and UTP were detected by spectrophotometry at 254 nm and their recoveries ranged from 97 to 100%. In the course of the reaction, a correlation was observed between the reduction in the area of the substrate peak and the occurrence of product peak(s). This highly reproducible method for enzyme assay is fast since no intermediate reaction mixture is required.
-
ndp sugars floridoside and floridean starch levels in relation to activities of udp glucose pyrophosphorylase and udp glucose 4 epimerase in Solieria chorda l is rhodophyceae under experimental conditions
Phycological Research, 2001Co-Authors: Fabienne Goulard, Eric Deslandes, M Diouris, Geraldine Le Corre, Jeanyves FlochAbstract:SUMMARY Under limited nutrient availability (i.e. unenriched sea-water) and under 75 mol photons m–2 s–1 irradiance 12:12 LD, thalli of Solieria chordalis J. Agardh accumulated floridean starch and floridoside. When they were transferred into nutrient-enriched seawater (150 umol L−1 NO31- and 7 umol L−1 P043i at 35 umol photons m−2 s−1 in irradiance 12:12 LD, starch and floridoside levels decreased. The main nucleotide diphosphate (NDP) sugars (i.e. UDP-glucose, UDP-galactose and ADP-glucose) and the activities of UDP-glucose pyrophosphorylase [Enzyme Code (EC) 2.7.7.9] and UDP-glucose-4-epimerase (EC 5.1.3.2) were measured under these controlled culture conditions. Both UDP-glucose and UDP-galactose in the thal l i increased under conditions known to favor the accumulation of floridean starch and floridoside, whereas they decreased under conditions leading to floridean starch and floridoside breakdown. On the other hand, ADP-glucose level only varied slightly. Although UDP-glucose pyrophosphorylase activity rose under conditions of floridean starch synthesis, little variation was observed in UDP-glucose-4-epimerase activity. These results suggest a possible enzymatic regulation of the NDP-sugar and carbohydrate pool in which UDP-glucose pyrophosphorylase would play a major role.
-
floridean starch and carrageenan contents as responses of the red alga Solieria chordalis to culture conditions
European Journal of Phycology, 1999Co-Authors: Isabelle Fournet, Eric Deslandes, M Diouris, M Zinoun, Jeanyves FlochAbstract:The influence of culture conditions on the biosynthesis and degradation of polysaccharides (starch and carrageenan) in the red alga Solieria chordalis was investigated. Following 1 week of incubation in the dark, the alga synthesized floridean starch at two irradiances, 50 and 480 μmol photons m−2 s−1 (400–700 nm), in nutrient-unenriched seawater. Upon transfer of these cultured algae to nutrient-enriched seawater at 50 μmol photons m−2 s−1, the stored floridean starch was found to break down. 14C labelling revealed that during floridean starch breakdown the tracer was transferred to carrageenan. These results are interpreted as carbon transfer from stored floridean starch for use in carrageenan biosynthesis.
-
nucleotides nucleoside sugars and udp glucose 4 epimerase activity in the iota carrageenophytes Solieria chordalis and calliblepharis jubata rhodophyceae
European Journal of Phycology, 1999Co-Authors: Fabienne Goulard, Eric Deslandes, M Diouris, Jeanyves FlochAbstract:The nucleotides and nucleoside diphosphate monosaccharides (NDP-sugars), UDP-galactose, UDP-glucose, UDP, ATP, ADP, ADPglucose, AMP and NAD, some of which are involved in carrageenan biosynthesis, were identified in the two carrageenophytes Calliblepharis jubata and Solieria chordalis and quantified by high-performance liquid chromatography (HPLC). Of several extraction procedures evaluated, a freeze-thaw step followed by formic acid (1 M) extraction was the preferred method. The pool size of nucleotides and NDP-sugars differed between the two algal species, which may be related to their growth state. Calliblepharis, which was not growing actively, had larger pools than Solieria, which was in an active growth phase. Using an HPLC method UDP-d-glucose-4-epimerase activity was measured in cell-free extracts of both algae, for the first time in a carrageenophyte.
Eric Deslandes - One of the best experts on this subject based on the ideXlab platform.
-
effects of high hydrostatic pressure and polysaccharidases on the extraction of antioxidant compounds from red macroalgae palmaria palmata and Solieria chordalis
Journal of Food Engineering, 2019Co-Authors: Shyam Suwal, Lucie Beaulieu, Alice Marciniak, Helene Jacques, Veronique Perreault, Éric Tamigneaux, Laurent Bazinet, Eric Deslandes, Alain DoyenAbstract:Abstract A non-thermal high hydrostatic pressure (HHP) technology in combination with polysaccharidases is proposed as a novel approach to improve the phytochemical extraction from red macroalgae. Two macroalgae species, Palmaria palmata and Solieria chordalis, were hydrolyzed with cellulase and hemicellulase (separately or in combination) under HHP (400 MPa, 20 min). The HHP-assisted enzymatic treatment improved the extraction of specific molecules such as proteins, polyphenols and polysaccharides, but their effects are highly dependent on the macroalgae species. Consequently, the antioxidant activity of extracted fractions was improved by over 2.8 times for the treatment with HHP with hemicellulase. Antioxidant activity was highly correlated with polysaccharide (89%) and protein (83%) contents for S. chordalis, and with polyphenol (65%) for P. palmata. Our experiments demonstrated, for the first time, the potential of HHP-assisted enzymatic extraction of various phytochemicals from red macroalgae and the fact that their effects are highly dependent on the macroalgae species used.
-
Effects of high hydrostatic pressure and polysaccharidases on the extraction of antioxidant compounds from red macroalgae, Palmata palmata and Solieria chordalis
Journal of Food Engineering, 2019Co-Authors: Shyam Suwal, Lucie Beaulieu, Alice Marciniak, Helene Jacques, Veronique Perreault, Éric Tamigneaux, Laurent Bazinet, Eric Deslandes, Alain DoyenAbstract:A non-thermal high hydrostatic pressure (HHP) technology in combination with polysaccharidases is proposed as a novel approach to improve the phytochemical extraction from red macroalgae. Two macroalgae species, palmata palmata and Solieria chordalis, were hydrolyzed with cellulase and hemicellulase (separately or in combination) under HHP (400 MPa, 20 min). The HHP-assisted enzymatic treatment improved the extraction of specific molecules such as proteins, polyphenols and polysaccharides, but their effects are highly dependent on the macroalgae species. Consequently, the antioxidant activity of extracted fractions was improved by over 2.8 times for the treatment with HHP with hemicellulase. Antioxidant activity was highly correlated with polysaccharide (89%) and protein (83%) contents for S. chordalis, and with polyphenol (65%) for P. palmata. Our experiments demonstrated, for the first time, the potential of HHP-assisted enzymatic extraction of various phytochemicals from red macroalgae and the fact that their effects are highly dependent on the macroalgae species used.
-
Carrageenan from Solieria chordalis (Gigartinales): Structural analysis and immunological activities of the low molecular weight fractions
Carbohydrate Polymers, 2010Co-Authors: Stéphanie Bondu, Eric Deslandes, Marie-sophie Fabre, Christian Berthou, Yu Guang LiAbstract:The investigations reported here are about a carrageenan extracted from the abundant red alga, Solieria chordalis (Gigartinales) settled along the coasts of Brittany. Its structural features were characterized by GC―MS, 13C NMR and FTIR spectroscopies. The structural components of this polysaccharide are mainly a (DA2S-G4S)-type structure in association with methylated-L-carrageenan, pyruvated α-carrageenan and the minor precursor, v-carrageenen, in small amounts. The relative molecular weight of the native polysaccharide was estimated by LP-GPC as 913 kDa. The low molecular weight fractions (below 20 kDa) obtained by free-radical depolymerization and mild-acid hydrolysis presented substitution patterns similar to those of the native polysaccharide. These fractions proved to be devoid of direct cytotoxicity on Daudi (human Burkitt's lymphoma), Jurkat (human leukaemic T-cell lymphoblast) and K562 (human chronic myelogenous leukaemia) cells lines. On the other hand, they showed great immunostimulating properties: enhancement of neutrophil phagocytosis, cytotoxicity by natural killer cells, antibody-dependent cell cytotoxicity and stimulation of lymphocyte proliferation. Further to these investigations, it could be worth using the low molecular weight fractions of carrageenan from the red alga, S. chordalis, in immunotherapeutic approaches to cancer treatment.
-
Oligocarrageenans and tissue-dependant oxidative burst in Solieria chordalis (Rhodophyceae, Gigartinales)
Phycological Research, 2008Co-Authors: Erwan Ar Gall, Laurence Cornec, Eric DeslandesAbstract:The release of hydrogen peroxide by thallus fragments of the rhodophycean Solieria chordalis (C. Agardh) J. Agardh has been documented both in the presence and in the absence of oligosaccharides. Within 1 h, ramuli were able to release large amounts of peroxide in the absence of any chemical stress. Among potential elicitors tested, only degree of polymerization 1 (DP1) and DP7-8 oligo-iota-carrageenans stimulated defense mechanisms in both axes and ramuli as shown by the occurrence of an oxidative burst. Chopping of the tissues had no effect on the intensity of the burst, therefore suggesting that mainly cortical cell layers were involved in the process. After 5 min incubation, a dose of 125 mu g mL(-1) of an oligomeric mixture containing a large proportion of DP1 units proved to be sufficient to obtain a maximal response. The intensity of the burst was significantly higher with isolated ramuli than with pieces of the axis, with outer peroxide accumulations reaching 200 nmol g(-1) fresh weight of treated tissue. Altogether, our results show that S. chordalis is able to react to a simulated pathogen attack by an oxidative burst and that the capacity to carry out an oxidative burst is stronger in ramuli than in axes.
-
partial purification and characterization of udp glucose 4 epimerase from Solieria chordalis rhodophyceae
Botanica Marina, 2003Co-Authors: Fabienne Goulard, Eric Deslandes, P Pondaven, M Diouris, Jeanyves FlochAbstract:The partial purification of UDP-glucose-4-epimerase was carried out on the red macroalga Solieria chordalis. A 60-fold purification factor was obtained by chromatography on DEAE Protein-Pack 8 HR and Superose 12. Gel-filtration fast protein liquid chromatography (FPLC) showed that the enzyme had an apparent molecular mass of 130 kDa. The UDP-glucose-4-epimerase from Solieria chordalis does not require complementary NAD addition for activity, unlike the enzyme from some other organisms. Moreover, the optimal pH was at 8.5 and the apparent K M values for UDP-glucose and UDP-galactose were 87 μM and 630 μM, respectively.
Nathalie Bourgougnon - One of the best experts on this subject based on the ideXlab platform.
-
Environmentally Friendly Valorization of Solieria filiformis (Gigartinales, Rhodophyta) from IMTA Using a Biorefinery Concept
Marine drugs, 2018Co-Authors: Ana Peñuela, Gilles Bedoux, Nathalie Bourgougnon, Daniel Robledo, Emanuel Hernández-núñez, Yolanda Freile-pelegrínAbstract:Marine macroalgae (seaweed) are an excellent source of novel bioactive metabolites. The biorefinery concept applied to seaweed facilitates the extraction of many chemical constituents from the same biomass ensuring that the resource is used fully, generating few residues through a succession of extraction steps. In the present study, the biomass of the carragenophyte Solieria filiformis (Rhodophyta, Gigartinales) cultured in an integrated multi-trophic aquaculture (IMTA) system was evaluated to obtain valuable products by a biorefinery approach. Enzymatic-assisted extraction (EAE) and microwave-assisted extraction (MAE) were the eco-friendly technologies used to ensure an environmentally friendly valorization of the biomass. Three valuable products were successfully recovered: a water-soluble extract rich in proteins and sulfated polysaccharides suitable as a food supplement; a lipid fraction rich in polyunsaturated fatty acids (PUFAs) with potential to be used in the nutraceutical industry; and a pure ι-carrageenan with a powerful antiviral activity against Herpes simplex virus (EC 50 = 6.3 µg mL −1) comparable to the commercial antiviral acyclovir (EC 50 = 3.2-5.4 µg mL −1).
-
Radical scavenging activity of lipids from seaweeds isolated by solid-liquid extraction and supercritical fluids
Oléagineux Corps Gras Lipides, 2018Co-Authors: Nolwenn Terme, Romain Boulho, Jean-philippe Kucma, Nathalie Bourgougnon, Gilles BedouxAbstract:In vitro antioxidant activities of the lipid fractions from two selected seaweeds, Solieria chordalis and Sargassum muticum were investigated according to the extraction methods. The activity of neutral lipids, glycolipids and phospholipids, thanks to extraction by chloroform/methanol (1/1 v/v; CM) mixture, pure supercritical carbon dioxide (sc-CO2), supercritical carbon dioxide with 2% of ethanol (sc-CO2 + EtOH 2%) and supercritical carbon dioxide with 8% of ethanol as co-solvent (sc-CO2 + EtOH 8%), were studied using DPPH radical scavenging assays. All the lipid classes demonstrated a free radical scavenging activity at the concentration of 1 mg/ml. The best scavenging activity (86.6 +/- 5.7%) was obtained when the neutral lipid fraction was extracted from S. chordalis with a CM mixture. The neutral lipid fraction extracted with sc-CO2 showed a lower activity than those obtained with solvents. However, the addition of ethanol in sc-CO(2 )did not affect the antioxidant activity of neutral lipids fixed at around 16% of radical scavenging. For S. muticum, the activity of glycolipids (50.9 +/- 0.8%) and phospholipids (48.4 +/- 1.6%) obtained with sc-CO2 were twice as large as that of fractions obtained with CM, 29.6 +/- 3.4% and 28.0 +/- 4.2%, respectively. The activity of neutral lipids did not change with the extraction method with around 25% of radical scavenging. This is the first report of free radical scavenging activity of lipid classes obtained by supercritical carbon dioxide extraction from seaweeds.
-
Selective extraction of lipid classes from Solieria chordalis and Sargassum muticum using supercritical carbon dioxide and conventional solid–liquid methods
Journal of Applied Phycology, 2017Co-Authors: Nolwenn Terme, Romain Boulho, Melha Kendel, Jean-philippe Kucma, Gaëtane Wielgosz-collin, Nathalie Bourgougnon, Gilles BedouxAbstract:Lipid extraction yields and lipid class distribution from two seaweeds Solieria chordalis (Rhodophyceae) and Sargassum muticum (Phaeophyceae) were investigated using different extraction methods. Supercritical carbon dioxide extraction with or without co-solvent and chloroform/methanol extractions were applied to S. chordalis and S. muticum. Total lipid contents ranged between 0.13 and 2.96% dry weight (dw) and between 0.8 and 3.15% dw for S. chordalis and S. muticum, respectively. The best yields were obtained from chloroform/methanol mixture. A promising 1.21% dw yield was obtained from supercritical CO2 with 2% ethanol for S. chordalis. The proportion of the lipid classes drastically changed according to the method. By using the chloroform/methanol Bligh and Dyer mixture, the main fractions extracted from S. chordalis were neutral lipids (37%) and glycolipids (38%) whereas glycolipids (60%) were mainly isolated using pure supercritical CO2. An addition of ethanol (2%) as co-solvent enhanced the neutral lipid fraction up to 56%. The glycolipid fraction reached 82.2% of total lipids when S. muticum was treated by supercritical CO2, and 61.2 to 70.8% for chloroform/methanol procedures. More broadly, polar lipid ratio (glycolipids and phospholipids) was important in the brown seaweed with 86 to 95% of total lipid content. These results highlighted the relationships between the extraction process and the lipid class distribution.
-
antiherpetic hsv 1 activity of carrageenans from the red seaweed Solieria chordalis rhodophyta gigartinales extracted by microwave assisted extraction mae
Journal of Applied Phycology, 2017Co-Authors: Romain Boulho, Nathalie Bourgougnon, Daniel Robledo, Yolanda Freilepelegrin, Christel Marty, Gilles BedouxAbstract:Carrageenan yield, physicochemical properties, and antiviral activity of the carrageenan from Solieria chordalis (Rhodophyceae, Solieriaceae) harvested at the Brittany coast (France) were investigated. S. chordalis carrageenan was extracted by conventional and the microwave-assisted extraction (MAE) methods. The effect of different parameters during MAE extraction such as alkali concentration (0, 0.5 and 1% KOH), extraction time (10, 20, and 25 min) and temperature (90 and 105 °C) were evaluated. Native carrageenan extracted by MAE had the highest yield (29.3%) after 10 min at 90 °C. After alkali treatment, carrageenan yield ranged from 10.7 to 18.4%. No significant differences in the carrageenan yield were observed between MAE and conventional method under alkaline conditions. Chemical analysis and FTIR spectra revealed the presence of a predominant iota-carrageenan. Evaluation of the antiviral activity of S. chordalis carrageenan against HSV-1 (Herpes simplex virus type 1) showed a EC50 of the iota-carrageenans fractions in the range of 3.2 to 54.4 μg mL−1 (MOI 0.01 ID50 mL−1) without cytotoxicity in that range of concentrations.
-
fractionation of uv b absorbing molecules and of free radical scavenging compounds from Solieria chordalis by using centrifugal partition chromatography
Phytochemistry Letters, 2017Co-Authors: Romain Boulho, Nathalie Bourgougnon, Le J Roux, Le C Quemener, Gregoire Audo, Gilles BedouxAbstract:Abstract Liquid-liquid chromatography can lead to the isolation of compounds or enrichment of fractions. To investigate the structural characteristics of antioxidants and UV-B absorbing molecules, a preparative centrifugal partition chromatography (CPC) method was developed to prepare enriched bioactive fractions from the red seaweed Solieria chordalis (Gigartinales, Solieriaceae). After a methanolic extraction of dried Solieria chordalis and liquid-liquid separation using ethyl acetate/water (v/v), the ethyl acetate phase was submitted to CPC using Arizona Y of the quaternary solvent system composed of n-heptane/ethyl acetate/methanol/water (19/1/19/1, v/v) in ascending mode. Among the fifteen collected fractions, three fractions gave up to 23.50% of DPPH radical scavenging activity. The CPC fractionation was monitored by HPLC and, four compounds exhibited UV-B absorbing capacity regarding their absorption at 310 nm.
