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Clive R Taylor - One of the best experts on this subject based on the ideXlab platform.

  • new dimensions of Antigen Retrieval technique 28 years of development practice and expansion
    Applied Immunohistochemistry & Molecular Morphology, 2019
    Co-Authors: Shan Rong Shi, Yan Shi, Clive R Taylor
    Abstract:

    This review article summarized recent advances in the heat-induced Antigen Retrieval technique with numerous scientific fields in addition to immunohistochemistry. Particularly, proteomics including imaging mass spectrometry, extraction of proteins from formalin-fixed, paraffin-embedded (FFPE) tissues. Some novel approaches such as FFPE tissue-based renal immunopathology based on modified double heating protocols are also introduced in this review for further development. In general, the FFPE tissue housed in pathology worldwide is an invaluable treasure, and the simple method of heat-induced Antigen Retrieval is the gold key to open the door of this treasure.

  • Antigen Retrieval technique
    2017
    Co-Authors: Shan Rong Shi, Richard J Cote, Clive R Taylor
    Abstract:

    Development of the Antigen Retrieval (AR) technique, a simple method of boiling archival paraffin-embedded tissue sections in water to enhance the signal of immunohistochemistry (IHC), was the fruit of pioneering efforts guided by the philosophy of rendering IHC applicable to routine formalin-fixed, paraffin-embedded tissues for wide application of IHC in research and clinical pathology. On the basis of thousands of articles and many reviews, a book has recently been published that summarizes basic principles for practice and further development of the AR technique. Major topics with respect to several critical issues, such as the definition, application, technical principles, and further studies of the AR technique, are highlighted in this article. In particular, a further application of the heat-induced Retrieval approach for sufficient extraction of nucleic acids in addition to proteins, and standardization of routine IHC based on the AR technique in terms of a test battery approach, are also addressed. Furthermore, understanding the mechanism of the AR technique may shed light on facilitating the development of molecular morphology.

  • Antigen Retrieval in immunohistochemistry
    Journal of Histochemistry and Cytochemistry, 2014
    Co-Authors: Shan Rong Shi, Clive R Taylor
    Abstract:

    The Antigen Retrieval (AR) technique of boiling formalin-fixed, paraffin-embedded (FFPE) tissue sections in water is a highly effective method to render immunohistochemical (IHC) staining on FFPE tissue sections, which is a revolution in pathology and a milestone in IHC. The authors intend briefly to summarize developments in AR-IHC-based research and diagnostics, with particular emphasis on current challenges and future research directions based on our data and a wide literature review. This article includes basic principles and essential techniques of AR and several major issues in terms of standardization and quantitative IHC based on AR, extraction of nucleic acids and proteins from FFPE tissue for genomic and proteomic analysis, molecular mechanism of AR, and other related topics. Currently, the AR-IHC-based research is in developing, with describing our plan for future study, we wish that combining the proteomics approach with AR-IHC to establish a sophisticated platform for identifying biomarkers in personalized medicine.

  • Antigen Retrieval immunohistochemistry review and future prospects in research and diagnosis over two decades
    Journal of Histochemistry and Cytochemistry, 2011
    Co-Authors: Clive R Taylor, Shan Rong Shi, Yan Shi
    Abstract:

    As a review for the 20th anniversary of publishing the Antigen Retrieval (AR) technique in this journal, the authors intend briefly to summarize developments in AR-immunohistochemistry (IHC)–based research and diagnostics, with particular emphasis on current challenges and future research directions. Over the past 20 years, the efforts of many different investigators have coalesced in extending the AR approach to all areas of anatomic pathology diagnosis and research and further have led to AR-based protein extraction techniques and tissue-based proteomics. As a result, formalin-fixed paraffin-embedded (FFPE) archival tissue collections are now seen as a literal treasure of materials for clinical and translational research to an extent unimaginable just two decades ago. Further research in AR-IHC is likely to focus on tissue proteomics, developing a more efficient protocol for protein extraction from FFPE tissue based on the AR principle, and combining the proteomics approach with AR-IHC to establish a practical, sophisticated platform for identifying and using biomarkers in personalized medicine.

  • Antigen Retrieval immunohistochemistry based research and diagnostics
    TAEBDC-2013, 2010
    Co-Authors: Shan Rong Shi, Clive R Taylor
    Abstract:

    PREFACE. CONTRIBUTORS. PART I: RECENT ADVANCES IN Antigen Retrieval TECHNIQUES AND ITS APPLICATION. 1 STANDARDIZATION OF Antigen Retrieval TECHNIQUES BASED ON THE TEST BATTERY APPROACH (Shan-Rong Shi and Clive R. Taylor). 2 EXTENDED APPLICATION OF Antigen Retrieval TECHNIQUE IN IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION (Shan-Rong Shi and Clive R. Taylor). 3 EXTRACTION OF DNA/RNA FROM FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE BASED ON THE Antigen Retrieval PRINCIPLE (Shan-Rong Shi and Clive R. Taylor). PART II: STANDARDIZATION OF IMMUNOHISTOCHEMISTRY. 4 KEY ISSUES AND STRATEGIES OF STANDARDIZATION FOR QUANTIFIABLE IMMUNOHISTOCHEMISTRY (Shan-Rong Shi, Kevin A. Roth, and Clive R. Taylor). 5 STANDARDIZATION OF IMMUNOHISTOCHEMISTRY BASED ON Antigen Retrieval TECHNIQUE (Shan-Rong Shi and Clive R. Taylor). 6 STANDARD REFERENCE MATERIAL: CELL LINE DEVELOPMENT AND USE OF REFERENCE CELL LINES AS STANDARDS FOR EXTERNAL QUALITY ASSURANCE OF HER2 IHC AND ISH TESTING (Bharat Jasani, Vicky Reid, Colin Tristram, Jeremy Walker, Paul Scorer, Michael Morgan, John Bartlett, Merdol Ibrahim, and Keith Miller). 7 PEPTIDES AS IMMUNOHISTOCHEMISTRY CONTROLS (Steven A. Bogen and Seshi R. Sompuram). 8 STANDARD REFERENCE MATERIAL: PROTEINEMBEDDING TECHNIQUE AND DESIGN OF BAR CODE (Shan-Rong Shi, Jiang Gu, and Clive R. Taylor). 9 THE PROS AND CONS OF AUTOMATION FOR IMMUNOHISTOCHEMISTRY FROM THE PROSPECTIVE OF THE PATHOLOGY LABORATORY (David G. Hicks and Loralee McMahon). 10 IMAGE ANALYSIS IN IMMUNOHISTOCHEMISTRY (Alton D. Floyd). PART III TISSUE/CELL SAMPLE PREPARATION. 11 TISSUE CELL SAMPLE PREPARATION: LESSONS FROM THE Antigen Retrieval TECHNIQUE (Shan-Rong Shi and Clive R. Taylor). 12 MECHANISMS OF ACTION AND PROPER USE OF COMMON FIXATIVES (Richard W. Dapson). 13 CELL SAMPLE PREPARATION FOR CLINICAL CYTOPATHOLOGY: CURRENT STATUS AND FUTURE DEVELOPMENT (Yan Shi and Patricia G. Wasserman). 14 DESIGN OF A TISSUE SURROGATE TO EXAMINE ACCURACY OF PROTEOMIC ANALYSIS (Carol B. Fowler, Jeffrey T. Mason, and Timothy J. O'leary). PART IV: MOLECULAR MECHANISM OF Antigen Retrieval TECHNIQUE. 15 STUDY OF FORMALIN FIXATION AND HEAT-INDUCED Antigen Retrieval (Jeffrey T. Mason, Carol B. Fowler, and Timothy J. O'leary). 16 A LINEAR EPITOPES MODEL OF Antigen Retrieval (Steven A. Bogen and Seshi R. Sompuram). 17 PH OR IONIC STRENGTH OF Antigen Retrieval SOLUTION: A POTENTIAL ROLE FOR REFOLDING DURING HEAT TREATMENT (Shuji Yamashita). 18 COMMENTARY: FUTURE DIRECTIONS (Timothy J. O'Leary, Carol B. Fowler, David L. Evers, Robert E. Cunningham, and Jeffrey T. Mason). PART V: PROTEOMIC ANALYSIS OF PROTEIN EXTRACTED FROM TISSUE/CELLS. 19 TECHNIQUES OF PROTEIN EXTRACTION FROM FFPE TISSUE/CELLS FOR MASS SPECTROMETRY (Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason). 20 APPLICATION OF SHOTGUN PROTEOMICS TO FORMALIN-FIXED AND PARAFFIN-EMBEDDED TISSUES (Brian M. Balgley). 21 VISUALIZING PROTEIN MAPS IN TISSUE (Masahiro Mukai and Mitsutoshi Setou). 22 SYMBIOSIS OF IMMUNOHISTOCHEMISTRY AND PROTEOMICS: MARCHING TO A NEW ERA (Shan-Rong Shi, Brian M. Balgley, and Clive R. Taylor). APPENDIX RELATED LABORATORY PROTOCOLS. INDEX.

Shan Rong Shi - One of the best experts on this subject based on the ideXlab platform.

  • new dimensions of Antigen Retrieval technique 28 years of development practice and expansion
    Applied Immunohistochemistry & Molecular Morphology, 2019
    Co-Authors: Shan Rong Shi, Yan Shi, Clive R Taylor
    Abstract:

    This review article summarized recent advances in the heat-induced Antigen Retrieval technique with numerous scientific fields in addition to immunohistochemistry. Particularly, proteomics including imaging mass spectrometry, extraction of proteins from formalin-fixed, paraffin-embedded (FFPE) tissues. Some novel approaches such as FFPE tissue-based renal immunopathology based on modified double heating protocols are also introduced in this review for further development. In general, the FFPE tissue housed in pathology worldwide is an invaluable treasure, and the simple method of heat-induced Antigen Retrieval is the gold key to open the door of this treasure.

  • Antigen Retrieval technique
    2017
    Co-Authors: Shan Rong Shi, Richard J Cote, Clive R Taylor
    Abstract:

    Development of the Antigen Retrieval (AR) technique, a simple method of boiling archival paraffin-embedded tissue sections in water to enhance the signal of immunohistochemistry (IHC), was the fruit of pioneering efforts guided by the philosophy of rendering IHC applicable to routine formalin-fixed, paraffin-embedded tissues for wide application of IHC in research and clinical pathology. On the basis of thousands of articles and many reviews, a book has recently been published that summarizes basic principles for practice and further development of the AR technique. Major topics with respect to several critical issues, such as the definition, application, technical principles, and further studies of the AR technique, are highlighted in this article. In particular, a further application of the heat-induced Retrieval approach for sufficient extraction of nucleic acids in addition to proteins, and standardization of routine IHC based on the AR technique in terms of a test battery approach, are also addressed. Furthermore, understanding the mechanism of the AR technique may shed light on facilitating the development of molecular morphology.

  • Antigen Retrieval in immunohistochemistry
    Journal of Histochemistry and Cytochemistry, 2014
    Co-Authors: Shan Rong Shi, Clive R Taylor
    Abstract:

    The Antigen Retrieval (AR) technique of boiling formalin-fixed, paraffin-embedded (FFPE) tissue sections in water is a highly effective method to render immunohistochemical (IHC) staining on FFPE tissue sections, which is a revolution in pathology and a milestone in IHC. The authors intend briefly to summarize developments in AR-IHC-based research and diagnostics, with particular emphasis on current challenges and future research directions based on our data and a wide literature review. This article includes basic principles and essential techniques of AR and several major issues in terms of standardization and quantitative IHC based on AR, extraction of nucleic acids and proteins from FFPE tissue for genomic and proteomic analysis, molecular mechanism of AR, and other related topics. Currently, the AR-IHC-based research is in developing, with describing our plan for future study, we wish that combining the proteomics approach with AR-IHC to establish a sophisticated platform for identifying biomarkers in personalized medicine.

  • Antigen Retrieval immunohistochemistry review and future prospects in research and diagnosis over two decades
    Journal of Histochemistry and Cytochemistry, 2011
    Co-Authors: Clive R Taylor, Shan Rong Shi, Yan Shi
    Abstract:

    As a review for the 20th anniversary of publishing the Antigen Retrieval (AR) technique in this journal, the authors intend briefly to summarize developments in AR-immunohistochemistry (IHC)–based research and diagnostics, with particular emphasis on current challenges and future research directions. Over the past 20 years, the efforts of many different investigators have coalesced in extending the AR approach to all areas of anatomic pathology diagnosis and research and further have led to AR-based protein extraction techniques and tissue-based proteomics. As a result, formalin-fixed paraffin-embedded (FFPE) archival tissue collections are now seen as a literal treasure of materials for clinical and translational research to an extent unimaginable just two decades ago. Further research in AR-IHC is likely to focus on tissue proteomics, developing a more efficient protocol for protein extraction from FFPE tissue based on the AR principle, and combining the proteomics approach with AR-IHC to establish a practical, sophisticated platform for identifying and using biomarkers in personalized medicine.

  • Antigen Retrieval immunohistochemistry based research and diagnostics
    TAEBDC-2013, 2010
    Co-Authors: Shan Rong Shi, Clive R Taylor
    Abstract:

    PREFACE. CONTRIBUTORS. PART I: RECENT ADVANCES IN Antigen Retrieval TECHNIQUES AND ITS APPLICATION. 1 STANDARDIZATION OF Antigen Retrieval TECHNIQUES BASED ON THE TEST BATTERY APPROACH (Shan-Rong Shi and Clive R. Taylor). 2 EXTENDED APPLICATION OF Antigen Retrieval TECHNIQUE IN IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION (Shan-Rong Shi and Clive R. Taylor). 3 EXTRACTION OF DNA/RNA FROM FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE BASED ON THE Antigen Retrieval PRINCIPLE (Shan-Rong Shi and Clive R. Taylor). PART II: STANDARDIZATION OF IMMUNOHISTOCHEMISTRY. 4 KEY ISSUES AND STRATEGIES OF STANDARDIZATION FOR QUANTIFIABLE IMMUNOHISTOCHEMISTRY (Shan-Rong Shi, Kevin A. Roth, and Clive R. Taylor). 5 STANDARDIZATION OF IMMUNOHISTOCHEMISTRY BASED ON Antigen Retrieval TECHNIQUE (Shan-Rong Shi and Clive R. Taylor). 6 STANDARD REFERENCE MATERIAL: CELL LINE DEVELOPMENT AND USE OF REFERENCE CELL LINES AS STANDARDS FOR EXTERNAL QUALITY ASSURANCE OF HER2 IHC AND ISH TESTING (Bharat Jasani, Vicky Reid, Colin Tristram, Jeremy Walker, Paul Scorer, Michael Morgan, John Bartlett, Merdol Ibrahim, and Keith Miller). 7 PEPTIDES AS IMMUNOHISTOCHEMISTRY CONTROLS (Steven A. Bogen and Seshi R. Sompuram). 8 STANDARD REFERENCE MATERIAL: PROTEINEMBEDDING TECHNIQUE AND DESIGN OF BAR CODE (Shan-Rong Shi, Jiang Gu, and Clive R. Taylor). 9 THE PROS AND CONS OF AUTOMATION FOR IMMUNOHISTOCHEMISTRY FROM THE PROSPECTIVE OF THE PATHOLOGY LABORATORY (David G. Hicks and Loralee McMahon). 10 IMAGE ANALYSIS IN IMMUNOHISTOCHEMISTRY (Alton D. Floyd). PART III TISSUE/CELL SAMPLE PREPARATION. 11 TISSUE CELL SAMPLE PREPARATION: LESSONS FROM THE Antigen Retrieval TECHNIQUE (Shan-Rong Shi and Clive R. Taylor). 12 MECHANISMS OF ACTION AND PROPER USE OF COMMON FIXATIVES (Richard W. Dapson). 13 CELL SAMPLE PREPARATION FOR CLINICAL CYTOPATHOLOGY: CURRENT STATUS AND FUTURE DEVELOPMENT (Yan Shi and Patricia G. Wasserman). 14 DESIGN OF A TISSUE SURROGATE TO EXAMINE ACCURACY OF PROTEOMIC ANALYSIS (Carol B. Fowler, Jeffrey T. Mason, and Timothy J. O'leary). PART IV: MOLECULAR MECHANISM OF Antigen Retrieval TECHNIQUE. 15 STUDY OF FORMALIN FIXATION AND HEAT-INDUCED Antigen Retrieval (Jeffrey T. Mason, Carol B. Fowler, and Timothy J. O'leary). 16 A LINEAR EPITOPES MODEL OF Antigen Retrieval (Steven A. Bogen and Seshi R. Sompuram). 17 PH OR IONIC STRENGTH OF Antigen Retrieval SOLUTION: A POTENTIAL ROLE FOR REFOLDING DURING HEAT TREATMENT (Shuji Yamashita). 18 COMMENTARY: FUTURE DIRECTIONS (Timothy J. O'Leary, Carol B. Fowler, David L. Evers, Robert E. Cunningham, and Jeffrey T. Mason). PART V: PROTEOMIC ANALYSIS OF PROTEIN EXTRACTED FROM TISSUE/CELLS. 19 TECHNIQUES OF PROTEIN EXTRACTION FROM FFPE TISSUE/CELLS FOR MASS SPECTROMETRY (Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason). 20 APPLICATION OF SHOTGUN PROTEOMICS TO FORMALIN-FIXED AND PARAFFIN-EMBEDDED TISSUES (Brian M. Balgley). 21 VISUALIZING PROTEIN MAPS IN TISSUE (Masahiro Mukai and Mitsutoshi Setou). 22 SYMBIOSIS OF IMMUNOHISTOCHEMISTRY AND PROTEOMICS: MARCHING TO A NEW ERA (Shan-Rong Shi, Brian M. Balgley, and Clive R. Taylor). APPENDIX RELATED LABORATORY PROTOCOLS. INDEX.

Joe C. Adams - One of the best experts on this subject based on the ideXlab platform.

Richard J Cote - One of the best experts on this subject based on the ideXlab platform.

  • Antigen Retrieval technique
    2017
    Co-Authors: Shan Rong Shi, Richard J Cote, Clive R Taylor
    Abstract:

    Development of the Antigen Retrieval (AR) technique, a simple method of boiling archival paraffin-embedded tissue sections in water to enhance the signal of immunohistochemistry (IHC), was the fruit of pioneering efforts guided by the philosophy of rendering IHC applicable to routine formalin-fixed, paraffin-embedded tissues for wide application of IHC in research and clinical pathology. On the basis of thousands of articles and many reviews, a book has recently been published that summarizes basic principles for practice and further development of the AR technique. Major topics with respect to several critical issues, such as the definition, application, technical principles, and further studies of the AR technique, are highlighted in this article. In particular, a further application of the heat-induced Retrieval approach for sufficient extraction of nucleic acids in addition to proteins, and standardization of routine IHC based on the AR technique in terms of a test battery approach, are also addressed. Furthermore, understanding the mechanism of the AR technique may shed light on facilitating the development of molecular morphology.

  • dna extraction from archival formalin fixed paraffin embedded tissue sections based on the Antigen Retrieval principle heating under the influence of ph
    Journal of Histochemistry and Cytochemistry, 2002
    Co-Authors: Richard J Cote, Lin Wu, Ram H Datar, Clive R Taylor
    Abstract:

    During the course of diagnostic surgical pathology, pathologists have established a large collection of formalin-fixed, paraffin-embedded tissues that form invaluable resources for translational studies of cancer and a variety of other diseases. Accessibility of macromolecules in the fixed tissue specimens is a critical issue as exemplified by heat-induced Antigen Retrieval (AR) immunohistochemical (IHC) staining. On the basis of observations that heating may also enhance in situ hybridization (ISH) and the similarity of formalin-induced chemical modifications that occur in protein and in DNA, we designed a study to examine the efficiency of DNA extraction from archival formalin-fixed, paraffin-embedded tissues using an adaptation of the basic principles of the AR technique, i.e., heating the tissue under the influence of different pH values. Archival paraffin blocks of lymph nodes, tonsil, and colon were randomly selected. Each paraffin block was prepared in 34 microtubes. For each paraffin block, one tu...

  • Antigen Retrieval immunohistochemistry and molecular morphology in the year 2001
    Applied Immunohistochemistry & Molecular Morphology, 2001
    Co-Authors: Shan Rong Shi, Richard J Cote, Clive R Taylor
    Abstract:

    The impact of the Antigen Retrieval (AR) technique upon diagnostic immunohistochemistry (IHC) and upon research has been demonstrated by numerous of articles and more than a dozen major reviews. The specific aim of this survey of the field is to examine potential new approaches to Retrieval of nucleic acid and protein from archival paraffin-embedded tissue for molecular biology-based diagnostic procedures that form the basis of the emerging field of molecular morphology. Any new approach must incorporate the principles of standardization and improved reproducibility. The ultimate goal will be to understand the mechanisms of fixation (by formalin) and "unfixation" (by AR). In the interim, the diligent application of practical procedures that have been shown to be tried and true is the least that we must demand from ourselves and our laboratories.

  • Antigen Retrieval immunohistochemistry practice and development
    Journal of Histotechnology, 1997
    Co-Authors: Shan Rong Shi, Richard J Cote, Lillian Young, Clive R Taylor
    Abstract:

    AbstractThe Antigen Retrieval (AR) technique is a novel, simple, and reliable approach to routine immunohistochemistry (IHC) developed in 1991. It has increasingly been used in pathology and analytical morphology, with a wide fruitful field opened for both clinical and research projects. Hundreds of articles concerning AR-IHC have been published in a variety of journals all over the world. As a recently developed technique, the wide use of AR has raised numerous questions for further study. This review article focuses on several key points of the method and further developments of the AR technique that are different from some review articles published recently. (The J Histotechnol 20:145, 1997)

  • Antigen Retrieval immunohistochemistry past present and future
    Journal of Histochemistry and Cytochemistry, 1997
    Co-Authors: Shan Rong Shi, Richard J Cote, Clive R Taylor
    Abstract:

    The Antigen Retrieval (AR) technique, which is predominantly based on high-temperature heating of tissues, is used as a non-enzymatic pretreatment for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. It has been widely applied in pathology and analytical morphology. The existence of a growing body of literature on the AR technique raises a number of interesting issues for the further development of AR. These issues include the use of a "test battery" and the concept of "maximal Retrieval" applied to the selection of optimal test protocols for the standardization of AR.

Andreas H. Eckhard - One of the best experts on this subject based on the ideXlab platform.