The Experts below are selected from a list of 252 Experts worldwide ranked by ideXlab platform
Jinghao Zheng - One of the best experts on this subject based on the ideXlab platform.
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long segmental tracheal reconstruction in rabbits with pedicled tissue engineered trachea based on a 3d Printed Scaffold
Acta Biomaterialia, 2019Co-Authors: Hui Jing, Shoubao Wang, Wei Fu, Xiaoyang Zhang, Xiaomin He, Jinghao ZhengAbstract:Abstract Long-segmental tracheal defects constitute an intractable clinical problem, due to the lack of satisfactory tracheal substitutes for surgical reconstruction. Tissue engineered artificial substitutes could represent a promising approach to tackle this challenge. In our current study, tissue-engineered trachea, based on a 3D-Printed poly ( l -lactic acid) (PLLA) Scaffold with similar morphology to the native trachea of rabbits, was used for segmental tracheal reconstruction. The 3D-Printed Scaffolds were seeded with chondrocytes obtained from autologous auricula, dynamically pre-cultured in vitro for 2 weeks, and pre-vascularized in vivo for another 2 weeks to generate an integrated segmental trachea organoid unit. Then, segmental tracheal defects in rabbits were restored by transplanting the engineered tracheal substitute with pedicled muscular flaps. We found that the combination of in vitro pre-culture and in vivo pre-vascularization successfully generated a segmental tracheal substitute with bionic structure and mechanical properties similar to the native trachea of rabbits. Moreover, the stable blood supply provided by the pedicled muscular flaps facilitated the survival of chondrocytes and accelerated epithelialization, thereby improving the survival rate. The segmental trachea substitute engineered by a 3D-Printed Scaffold, in vitro pre-culture, and in vivo pre-vascularization enhanced survival in an early stage post-operation, presenting a promising approach for surgical reconstruction of long segmental tracheal defects. Statement of Significance We found that the combination of in vitro pre-culture and in vivo pre-vascularization successfully generated a segmental tracheal substitute with bionic structure and mechanical properties similar to the native trachea of rabbits. Moreover, the stable blood supply provided by the pedicled muscular flaps facilitated the survival of chondrocytes and accelerated epithelialization, thereby improving the survival rate of the rabbits. The segmental trachea substitute engineered by a 3D-Printed Scaffold, in vitro pre-culture, and in vivo pre-vascularization enhanced survival in an early stage post-operation, presenting a promising approach for surgical reconstruction of long segmental tracheal defects.
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tissue engineered trachea from a 3d Printed Scaffold enhances whole segment tracheal repair
Scientific Reports, 2017Co-Authors: Hengyi Zhang, Wei Fu, Xiaomin He, Wei Dong, Bei Feng, Maolin Chen, Zhiwei Xu, Nevin Witman, Jinghao ZhengAbstract:Long segmental repair of trachea stenosis is an intractable condition in the clinic. The reconstruction of an artificial substitute by tissue engineering is a promising approach to solve this unmet clinical need. 3D printing technology provides an infinite possibility for engineering a trachea. Here, we 3D Printed a biodegradable reticular polycaprolactone (PCL) Scaffold with similar morphology to the whole segment of rabbits’ native trachea. The 3D-Printed Scaffold was suspended in culture with chondrocytes for 2 (Group I) or 4 (Group II) weeks, respectively. This in vitro suspension produced a more successful reconstruction of a tissue-engineered trachea (TET), which enhanced the overall support function of the replaced tracheal segment. After implantation of the chondrocyte-treated Scaffold into the subcutaneous tissue of nude mice, the TET presented properties of mature cartilage tissue. To further evaluate the feasibility of repairing whole segment tracheal defects, replacement surgery of rabbits’ native trachea by TET was performed. Following postoperative care, mean survival time in Group I was 14.38 ± 5.42 days, and in Group II was 22.58 ± 16.10 days, with the longest survival time being 10 weeks in Group II. In conclusion, we demonstrate the feasibility of repairing whole segment tracheal defects with 3D Printed TET.
Aldrik H. Velders - One of the best experts on this subject based on the ideXlab platform.
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microfluidic devices simple 3d Printed Scaffold removal method for the fabrication of intricate microfluidic devices adv sci 9 2015
Advanced Science, 2015Co-Authors: Vittorio Saggiomo, Aldrik H. VeldersAbstract:ESCARGOT (Embedded Scaffold RemovinG Open Technology) is a novel and simple methodology for the manufacturing of complex microfluidic devices in a single block of polydimethylsiloxane, developed by V. Saggiomo and A. H. Velders in article number 1500125. Dissolvable Scaffolds are used for the creation of microfluidic channels without the use of clean rooms, and integration of external elements directly in the microfluidic device is shown to be easy.
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simple 3d Printed Scaffold removal method for the fabrication of intricate microfluidic devices
Advanced Science, 2015Co-Authors: Vittorio Saggiomo, Aldrik H. VeldersAbstract:An easy and cheap fabrication method for intricate polydimethylsiloxane microfluidic devices is presented. The acrylonitrile butadiene styrene Scaffold-removal method uses cheap, off-the-shelf materials and equipment for the fabrication of intricate microfluidic devices. The versatility of the method is proven by the fabrication of 3D multilayer, ship-in-a-bottle, selective heating, sensing, and NMR microfluidic devices. The methodology is coined ESCARGOT: Embedded Scaffold RemovinG Open Technology.
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Simple 3D Printed Scaffold-Removal Method for the Fabrication of Intricate Microfluidic Devices
Advanced Science, 2015Co-Authors: Vittorio Saggiomo, Aldrik H. VeldersAbstract:... 3D printing technology, the ABS Scaffold-removal method is simple and cheap compared to the current fabrication methods , yet powerful and ... Because of the multidisciplinary fields of applications we can envision, the simplicity of this method , coined ESCARGOT , is of ... \n
Su A Park - One of the best experts on this subject based on the ideXlab platform.
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development of a three dimensionally Printed Scaffold grafted with bone forming peptide 1 for enhanced bone regeneration with in vitro and in vivo evaluations
Journal of Colloid and Interface Science, 2019Co-Authors: Il Keun Kwon, Yoo Seob Shin, Su A ParkAbstract:Abstract Defects in bone are some of the most difficult injuries to treat. Biomimetic Scaffolds represent a promising approach for successful bone tissue regeneration. In this study, a three-dimensional (3D) Scaffold with osteo-inductive functionality was designed and assayed both in-vitro and in-vivo. Bone formation peptide-1 (BFP1), an osteo-promoting specific peptide, was covalently bound to a 3D Printed polycaprolactone (PCL) Scaffold using polydopamine (DOPA). The amount of BFP1 immobilized on the surface was found to increase depending on the BFP1 concentration of the loading solution. To observe the biological effects of the 3D Scaffolds, human tonsil-derived mesenchymal stem cells (hTMSCs) were isolated. The cells were cultured on the Scaffolds and observed to rapidly differentiate into osteoblast-like cells with osteo-promoting capabilities. The Scaffolds were implanted in a rabbit calvarial defect model for 8 weeks and successfully stimulated both vessel and bone regeneration. Osteo-promoting 3D Scaffolds may provide a safer and more efficient approach for bone repair and remodelling in regenerative medicine.
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fabrication of 3d Printed pcl peg polyblend Scaffold using rapid prototyping system for bone tissue engineering application
Journal of Bionic Engineering, 2018Co-Authors: Su A Park, Ji Min Seok, Il Keun KwonAbstract:Three-dimensional (3D) printing is a novel process used to manufacture bone tissue engineered Scaffolds. This process allows for easy control of the architecture at the micro structure. However, the Scaffold properties are typically limited in terms of cellular activity at the Scaffold surface due to the Printed materials properties. In this study, we developed a polycaprolactone (PCL) blended with polyethylene glycol (PEG) 3D Printed Scaffold using a rapid prototyping system. The manufactured Scaffolds were then washed out to form small pores on the surface in order to improve the Scaffolds hydrophilicity. We analyzed the resultant material by using Scanning Electron Microscopy (SEM), water absorption, water contact angle, in vitro WST-1, and the Bradford assay. Additionally, cells incubated on the fabricated Scaffolds were visualized by Confocal Laser Scanning Microscopy (CLSM). The developed Scaffolds exhibited small pores on the strand surface which served to increase hydrophilicity as well as improve cellular proliferation and increase total protein content. Our findings suggest that the presence of small pores on the Scaffolds can be used as an effective tool for improving implant cellular interaction. This research indicates that these modified Scaffolds can be considered useful for bone tissue engineering applications to improve human health.
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Fabrication of 3D Printed PCL/PEG Polyblend Scaffold Using Rapid Prototyping System for Bone Tissue Engineering Application
Journal of Bionic Engineering, 2018Co-Authors: Su A Park, Ji Min Seok, Il Keun KwonAbstract:Three-dimensional (3D) printing is a novel process used to manufacture bone tissue engineered Scaffolds. This process allows for easy control of the architecture at the micro structure. However, the Scaffold properties are typically limited in terms of cellular activity at the Scaffold surface due to the Printed materials properties. In this study, we developed a polycaprolactone (PCL) blended with polyethylene glycol (PEG) 3D Printed Scaffold using a rapid prototyping system. The manufactured Scaffolds were then washed out to form small pores on the surface in order to improve the Scaffolds hydrophilicity. We analyzed the resultant material by using Scanning Electron Microscopy (SEM), water absorption, water contact angle, in vitro WST-1, and the Bradford assay. Additionally, cells incubated on the fabricated Scaffolds were visualized by Confocal Laser Scanning Microscopy (CLSM). The developed Scaffolds exhibited small pores on the strand surface which served to increase hydrophilicity as well as improve cellular proliferation and increase total protein content. Our findings suggest that the presence of small pores on the Scaffolds can be used as an effective tool for improving implant cellular interaction. This research indicates that these modified Scaffolds can be considered useful for bone tissue engineering applications to improve human health.
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serial analysis of tracheal restenosis after 3d Printed Scaffold implantation recruited inflammatory cells and associated tissue changes
Tissue Engineering and Regenerative Medicine, 2017Co-Authors: Roza Khalmuratova, Su A Park, Eun Jae Chung, Hyun Woo Shin, Seong Keun KwonAbstract:Tracheal restenosis is a major obstacle to successful tracheal replacement, and remains the greatest challenge in tracheal regeneration. However, there have been no detailed investigations of restenosis. The present study was performed to analyze the serial changes in recruited inflammatory cells and associated histological changes after tracheal Scaffold implantation. Asymmetrically porous Scaffolds, which successfully prevented tracheal stenosis in a partial trachea defect model, designed with a tubular shape by electrospinning and reinforced by 3D-printing to reconstruct 2-cm circumferential tracheal defect. Serial rigid bronchoscopy, micro-computed tomography, and histology [H&E, Masson’s Trichrome, IHC against α-smooth muscle actin (α-SMA)] were performed 1, 4, and 8 weeks after transplantation. Progressive stenosis developed especially at the site of anastomosis. Neutrophils were the main inflammatory cells recruited in the early stage, while macrophage infiltration increased with time. Recruitment of fibroblasts peaked at 4 weeks and deposition of α-SMA increased from 4 weeks and was maintained through 8 weeks. During the first 8 weeks post-transplantation, neutrophils and macrophages played significant roles in restenosis of the trachea. Antagonists to these would be ideal targets to reduce restenosis and thus play a pivotal role in successful tracheal regeneration.
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segmental tracheal reconstruction by 3d Printed Scaffold pivotal role of asymmetrically porous membrane
Laryngoscope, 2016Co-Authors: Su A Park, Se Heang Oh, Seong Keun KwonAbstract:Objectives/Hypothesis Three-dimensional (3D) Printed Scaffold for tracheal reconstruction can substitute the conventional treatment of tracheal stenosis. This study investigated the survival outcomes of segmental tracheal reconstruction using 3D Printed polycaprolactone (PCL) Scaffold with or without asymmetrically porous membrane in rabbit animal model. Study Design Animal study. Methods Six mature New Zealand white rabbits were categorized into two groups (three animals for each) according to the procedures they received: tracheal reconstruction using 3D Printed PCL Scaffold without asymmetrically porous membrane (group 1) versus with asymmetrically porous membrane (group 2). We compared the endoscopic findings of tracheal lumen, radiologic assessment using microcomputed tomography (CT) scanner and histologic findings. Overall survival duration after procedure was compared in both groups. Results The survival of group 2 was longer than group 1 (21, 37, 46 days vs. 4, 10, 12 days, respectively). Although mucosal regeneration in tracheal lumen was not full enough in both groups, the patency was well maintained in group 2. Micro-CT and histologic analysis showed that there were tracheal narrowing in the whole length in group 1, whereas only the anastomosis site was stenotic in group 2. Conclusion Asymmetrically porous membrane reinforced by 3D Printed mesh is promising as a 360-degree tracheal substitute with comparable survival and luminal patency. Further study is necessary to minimize the narrowing of the anastomosis site and improve the mucosal regeneration for longer survival. Level of Evidence NA. Laryngoscope, 2015
Zhiwei Xu - One of the best experts on this subject based on the ideXlab platform.
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tissue engineered trachea from a 3d Printed Scaffold enhances whole segment tracheal repair in a goat model
Journal of Tissue Engineering and Regenerative Medicine, 2019Co-Authors: Qian Wang, Hengyi Zhang, Bei Feng, Maolin Chen, Nevin Witman, Jialing Zhang, Yanhui Huang, Yumin Zhong, Wei Wang, Zhiwei XuAbstract:: Traditional treatment therapies for tracheal stenosis often cause severe post-operative complications. To solve the current difficulties, novel and more suitable long-term treatments are needed. A whole-segment tissue-engineered trachea (TET) representing the native goat trachea was 3D Printed using a poly(caprolactone) (PCL) Scaffold engineered with autologous auricular cartilage cells. The TET underwent mechanical analysis followed by in vivo implantations in order to evaluate the clinical feasibility and potential. The 3D-Printed Scaffolds were successfully cellularized, as observed by scanning electron microscopy. Mechanical force compression studies revealed that both PCL Scaffolds and TETs have a more robust compressive strength than does the native trachea. In vivo implantation of TETs in the experimental group resulted in significantly higher mean post-operative survival times, 65.00 ± 24.01 days (n = 5), when compared with the control group, which received autologous trachea grafts, 17.60 ± 3.51 days (n = 5). Although tracheal narrowing was confirmed by bronchoscopy and computed tomography examination in the experimental group, tissue necrosis was only observed in the control group. Furthermore, an encouraging epithelial-like tissue formation was observed in the TETs after transplantation. This large animal study provides potential preclinical evidence around the employment of an orthotopic transplantation of a whole 3D-Printed TET.
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tissue engineered trachea from a 3d Printed Scaffold enhances whole segment tracheal repair
Scientific Reports, 2017Co-Authors: Hengyi Zhang, Wei Fu, Xiaomin He, Wei Dong, Bei Feng, Maolin Chen, Zhiwei Xu, Nevin Witman, Jinghao ZhengAbstract:Long segmental repair of trachea stenosis is an intractable condition in the clinic. The reconstruction of an artificial substitute by tissue engineering is a promising approach to solve this unmet clinical need. 3D printing technology provides an infinite possibility for engineering a trachea. Here, we 3D Printed a biodegradable reticular polycaprolactone (PCL) Scaffold with similar morphology to the whole segment of rabbits’ native trachea. The 3D-Printed Scaffold was suspended in culture with chondrocytes for 2 (Group I) or 4 (Group II) weeks, respectively. This in vitro suspension produced a more successful reconstruction of a tissue-engineered trachea (TET), which enhanced the overall support function of the replaced tracheal segment. After implantation of the chondrocyte-treated Scaffold into the subcutaneous tissue of nude mice, the TET presented properties of mature cartilage tissue. To further evaluate the feasibility of repairing whole segment tracheal defects, replacement surgery of rabbits’ native trachea by TET was performed. Following postoperative care, mean survival time in Group I was 14.38 ± 5.42 days, and in Group II was 22.58 ± 16.10 days, with the longest survival time being 10 weeks in Group II. In conclusion, we demonstrate the feasibility of repairing whole segment tracheal defects with 3D Printed TET.
Hengyi Zhang - One of the best experts on this subject based on the ideXlab platform.
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tissue engineered trachea from a 3d Printed Scaffold enhances whole segment tracheal repair in a goat model
Journal of Tissue Engineering and Regenerative Medicine, 2019Co-Authors: Qian Wang, Hengyi Zhang, Bei Feng, Maolin Chen, Nevin Witman, Jialing Zhang, Yanhui Huang, Yumin Zhong, Wei Wang, Zhiwei XuAbstract:: Traditional treatment therapies for tracheal stenosis often cause severe post-operative complications. To solve the current difficulties, novel and more suitable long-term treatments are needed. A whole-segment tissue-engineered trachea (TET) representing the native goat trachea was 3D Printed using a poly(caprolactone) (PCL) Scaffold engineered with autologous auricular cartilage cells. The TET underwent mechanical analysis followed by in vivo implantations in order to evaluate the clinical feasibility and potential. The 3D-Printed Scaffolds were successfully cellularized, as observed by scanning electron microscopy. Mechanical force compression studies revealed that both PCL Scaffolds and TETs have a more robust compressive strength than does the native trachea. In vivo implantation of TETs in the experimental group resulted in significantly higher mean post-operative survival times, 65.00 ± 24.01 days (n = 5), when compared with the control group, which received autologous trachea grafts, 17.60 ± 3.51 days (n = 5). Although tracheal narrowing was confirmed by bronchoscopy and computed tomography examination in the experimental group, tissue necrosis was only observed in the control group. Furthermore, an encouraging epithelial-like tissue formation was observed in the TETs after transplantation. This large animal study provides potential preclinical evidence around the employment of an orthotopic transplantation of a whole 3D-Printed TET.
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tissue engineered trachea from a 3d Printed Scaffold enhances whole segment tracheal repair
Scientific Reports, 2017Co-Authors: Hengyi Zhang, Wei Fu, Xiaomin He, Wei Dong, Bei Feng, Maolin Chen, Zhiwei Xu, Nevin Witman, Jinghao ZhengAbstract:Long segmental repair of trachea stenosis is an intractable condition in the clinic. The reconstruction of an artificial substitute by tissue engineering is a promising approach to solve this unmet clinical need. 3D printing technology provides an infinite possibility for engineering a trachea. Here, we 3D Printed a biodegradable reticular polycaprolactone (PCL) Scaffold with similar morphology to the whole segment of rabbits’ native trachea. The 3D-Printed Scaffold was suspended in culture with chondrocytes for 2 (Group I) or 4 (Group II) weeks, respectively. This in vitro suspension produced a more successful reconstruction of a tissue-engineered trachea (TET), which enhanced the overall support function of the replaced tracheal segment. After implantation of the chondrocyte-treated Scaffold into the subcutaneous tissue of nude mice, the TET presented properties of mature cartilage tissue. To further evaluate the feasibility of repairing whole segment tracheal defects, replacement surgery of rabbits’ native trachea by TET was performed. Following postoperative care, mean survival time in Group I was 14.38 ± 5.42 days, and in Group II was 22.58 ± 16.10 days, with the longest survival time being 10 weeks in Group II. In conclusion, we demonstrate the feasibility of repairing whole segment tracheal defects with 3D Printed TET.