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Kevin D Hyde - One of the best experts on this subject based on the ideXlab platform.
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guignardia phyllosticta species on banana
Cryptogamie Mycologie, 2010Co-Authors: N F Wulandari, Chaiwat Toanun, Kamelia A Abdelsalam, Kevin D HydeAbstract:Guignardia musae is the reported causal agent of freckle disease of banana. The epithet has, however, been introduced on three separate occasions and only one name is valid. We therefore investigated this problem. We examined the types of G. musae Racib., G. musae F. Stevens and G. musae Syd. & P. Syd. and also made fresh collections from banana in northern Thailand. Guignardia musae Racib. is the earliest name and takes precedence over the other two names which are hononyms. G. musae F. Stevens is a different species and therefore a new name G. stevensii Wulandari & K.D. Hyde is introduced to accommodate it. The name G. sydowiana Trotter has previously been introduced to accommodate G. musae Syd. & P Syd.; type material is, however, depauperate. Guignardia musicola Wulandari, L. Cai & K.D. Hyde sp. nov. is introduced as a new species from Thailand. The three species from banana are compared and their differences described.
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endophytic fungi of wild banana musa acuminata at doi suthep pui national park thailand
Fungal Biology, 2001Co-Authors: Wipornpan Photita, Saisamorn Lumyong, P Lumyong, Kevin D HydeAbstract:Endophytic fungi were isolated from 7500 samples of wild Musa acuminata collected from five sites at Doi Suthep Pui National Park, Thailand during December 1998 to July 1999. Overall colonization rates from surface sterilized tissues were 56.5, 48.9, 48, 47.9 and 41.7% for the Medicinal Plant Garden, Ban Suthep, Queen Sirikit Botanic Garden, San Gu, and Montatarn waterfall sites respectively. Sixty-one different fungal taxa were isolated. Fewer isolates were recovered from younger than older samples. Xylariaceous taxa and Guignardia cocoicola were the most frequently isolated endophytes from leaves and were either absent or rare in midrib, petiole and pseudostem. Colletotrichum gloeosporioides, C. musae, Guignardia cocoicola, various sterile mycelia and xylariaceous spp. were common at all sites. The endophyte fungal communities at the five sites were found to differ. Deightoniella torulosa was the most frequent isolate at the Ban Suthep site and was either absent or rare at other sites. Colletotrichum species were most common in the midribs and petioles at all sites, while Pyriculariopsis parasitica and Dactylaria sp. were most common in the pseudostems. The endophyte communities isolated from M. acuminata in this study are compared with those from previous studies on tropical hosts. Several of the endophytes isolated are established pathogens of banana and provide support for the hypothesis that some endophytes are latent pathogens. The diversity of fungi on banana is discussed in relation to global estimates of numbers of fungus species.
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guignardia candeloflamma sp nov causing leaf spots of pinanga spp
Fungal Biology, 1995Co-Authors: Jane Frohlich, Kevin D HydeAbstract:Guignardia candeloflamma sp. nov. is the cause of leaf spots on a species of Pinanga in Australia (north Queensland) and an unidentified palm in Irian Jaya. The taxon differs from previously described species of Guignardia from palms, with which it is compared. Guignardia candeloflamma has unusual appendages and these are illustrated with differential interference micrographs.
Antonio De Goes - One of the best experts on this subject based on the ideXlab platform.
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molecular and pathogenic study of guignardia spp isolates associated to different hosts
Ai Magazine, 2014Co-Authors: Ester Wickert, Andressa De Souza, Rodrigo Matheus Pereira, Luciano Takeshi Kishi, Eliana Gertrudes De Macedo Lemos, Antonio De GoesAbstract:Fungi of Guignardia genus are commonly isolated from different plant species and most of the time, they are characterized as endophytes. However, some species of this genus, like G. citricarpa and G. psidii are known as causal agents of serious diseases that affect important crops such as Citrus Black Spot and guava fruit rot, respectively. They are also responsible for diseases that cause foliar spots in different fruit species and also in other crops, but cause minor damages. Despite evidences that G. mangiferae colonizes different plant species, there are few studies about its genetic diversity associated with different hosts. This work has the objective to characterize Guignardia isolates obtained from different hosts and tissues by RAPD, fAFLP and DNA sequence of ITS1-5.8S-ITS2 region, as well as to develop pathogenicity tests through cross inoculation in citrus and guava fruits. It was observed that molecular markers were able to discriminate isolates of different Guignardia species. Pathogenicity tests showed that G. citricarpa caused CBS symptoms on citrus fruits, but it did not produce any symptoms in guava fruits. G. mangiferae isolates were able to cause rot symptoms on guava fruits, but they have not produced any symptoms on citrus fruits. Guignardia isolates obtained from mango leaves that have not been classified in species have not presented any symptoms in citrus and guava fruits. Although G. mangiferae is commonly isolated asymptomatically in different plants, this work supports the evidence that this species has a latent pathogen behavior, at least for guava plants.
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genetic diversity and population differentiation of guignardia mangiferae from tahiti acid lime
The Scientific World Journal, 2012Co-Authors: Ester Wickert, Andressa De Souza, Luciano Takeshi Kishi, Eliana Gertrudes De Macedo Lemos, Antonio De GoesAbstract:Among the citrus plants, “Tahiti” acid lime is known as a host of G. mangiferae fungi. This species is considered endophytic for citrus plants and is easily isolated from asymptomatic fruits and leaves. G. mangiferae is genetically related and sometimes confused with G. citricarpa which causes Citrus Black Spot (CBS). “Tahiti” acid lime is one of the few species that means to be resistant to this disease because it does not present symptoms. Despite the fact that it is commonly found in citric plants, little is known about the populations of G. mangiferae associated with these plants. Hence, the objective of this work was to gain insights about the genetic diversity of the G. mangiferae populations that colonize “Tahiti” acid limes by sequencing cistron ITS1-5.8S-ITS2. It was verified that “Tahiti” acid lime plants are hosts of G. mangiferae and also of G. citricarpa, without presenting symptoms of CBS. Populations of G. mangiferae present low-to-moderate genetic diversity and show little-to-moderate levels of population differentiation. As gene flow was detected among the studied populations and they share haplotypes, it is possible that all populations, from citrus plants and also from the other known hosts of this fungus, belong to one great panmictic population.
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control of guignardia citricarpa by bacillus subtilis and trichoderma spp
Revista Brasileira De Fruticultura, 2011Co-Authors: Katia Cristina Kupper, Elida Barbosa Correa, Cristiane Moretto, Wagner Bettiol, Antonio De GoesAbstract:The ability of isolates of Bacillus subtilis and Trichoderma spp. to control citrus black spot (CBS) was investigated in ´Natal´ sweet orange orchards. The first experiment was conducted during the 2001/2002 season and four isolates of B. subtilis (ACB-AP3, ACB-69, ACB-72 and ACB-77), applied every 28 days, alone or in combination were tested and compared with fungicide treatments. Two other experiments were carried out during the 2002/2003 season, where the same isolates of Bacillus and two isolates of Trichoderma (ACB-14 and ACB-40) were tested being applied every 28 days in the second experiment, and every 15 days in the third experiment. In the first experiment, the treatment with ACB-69 differed statistically from the control, but did not differ from other biological control agents or mixture of Bacillus isolates. In the second experiment, the treatments with ACB-69 and ACB-AP3 resulted in smaller disease index compared with the control treatment. However, this result was not repeated in the third experiment, where the isolates were applied every 15 days. Disease severity was high in both evaluated seasons and the fungicide treatment was the most effective for disease control.
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reacao de laranjeiras doces quanto a resistencia a guignardia citricarpa
Revista Brasileira De Fruticultura, 2010Co-Authors: Patricia Ferreira Cunha Sousa, Antonio De GoesAbstract:O objetivo deste trabalho foi determinar o comportamento de variedades de laranjas-doces quanto a resistencia a Guignardia citricarpa, agente causal da mancha preta dos citros (MPC). Os ensaios foram conduzidos em dois campos, nos municipios de Rincao e Tambau, SP. As mudas foram formadas em viveiro localizado na Estacao Experimental de Citricultura de Bebedouro-SP. Para tal, foram utilizadas borbulhas de plantas citricas existentes no Banco Ativo de Germoplasma de Citros da Estacao Experimental de Citricultura de Bebedouro (EECB), Bebedouro-SP. Avaliou-se a severidade da doenca por meio de escala de notas, de zero (ausencia de sintomas) a seis (sintomas severos). A partir de tais dados, foi calculado o valor do indice de doenca (ID). Em 2007, no experimento de Rincao, dentre as 65 variedades avaliadas, apenas 59 produziram frutos, sendo constatada ausencia de sintomas da doenca em Castellana, Maca e Olivelands. Nas demais variedades, os niveis de severidade variaram de 0,35 para Grada a 3,0 para China SRA-547. Para Tambau, os valores de severidade variaram de 0,40 para a variedade Cadenera a 2,46 para Pera. No ano de 2008, em Rincao, todas as variedades mostraram-se suscetiveis. Os niveis de severidade verificados variaram de 0,18 para a variedade Belladona, e 3,92 para Vera 97. Em Tambau, somente a variedade Navelina nao apresentou sintomas da MPC. Foi observado que, dentre as plantas que frutificaram, e com excecao daquelas cujos frutos mostraram-se assintomaticos, os valores de severidade variaram de 0,11 para a variedade Tua Mamede a 3,57 para Amares.
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relacoes filogeneticas e diversidade de isolados de guignardia spp oriundos de diferentes hospedeiros nas regioes its1 5 8s its2
Revista Brasileira De Fruticultura, 2009Co-Authors: Ester Wickert, Antonio De Goes, Andressa De Souza, Erico Leandro Da Silveira, Fernanda Dias Pereira, Eliana Gertrudes De Macedo Lemos, Davi RinaldoAbstract:Fungi of Guignardia genera are commonly isolated from different plant species and most of the time they are characterized as endophytics. However, some species of this genus, as G. citricarpa and G. psidii are known as causal agents of serious diseases that affect cultures, such as the Citrus Black Spot and the guava fruit rot, respectively. They are also responsible for diseases that cause foliar spots in different fruit species and also in other cultures. This work has the objective of isolate, identify and characterize the genetic diversity present among Guignardia isolates obtained from citrus, mango, guava, eucalyptus, Brazilian grape tree and Surinam cherry by analysis of DNA sequence from cistron ITS1-5,8SITS2. It was verified that the obtained isolates belong to G. mangiferae and G. citricarpa species. Two different Guignardia types, that were found in mango, could not be identified in species, and do not belong to none of the species deposited in GenBank. So, this work found that mango, guava, eucalyptus, Brazilian grape tree and Surinam cherry host only G. mangiferae, whereas citrus hosts G. mangiferae and G. citricarpa species. Mango hosts three different Guignardia, G. mangiferae and two others types that remain without identification of the species level. It was also verified that isolates of Guignardia obtained from guava fruit rot symptoms were identified as G. mangiferae.
Anne-claire Baudoux - One of the best experts on this subject based on the ideXlab platform.
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First Viruses Infecting the Marine Diatom Guinardia delicatula.
Frontiers in Microbiology, 2019Co-Authors: Laure Arsenieff, Nathalie Simon, Erwan Corre, Florence Le Gall, Fabienne Rigaut-jalabert, Samuel Chaffron, Estelle Bigeard, Anne-claire BaudouxAbstract:The marine diatom Guinardia delicatula is a cosmopolitan species that dominates seasonal blooms in the English Channel and the North Sea. Several eukaryotic parasites are known to induce the mortality of this species. Here, we report the isolation and characterization of the first viruses that infect G. delicatula. Viruses were isolated from the Western English Channel (SOMLIT-Astan station) during the late summer bloom decline of G. delicatula. A combination of laboratory approaches revealed that these lytic viruses (GdelRNAV) are small tailless particles of 35-38 nm in diameter that replicate in the host cytoplasm where both unordered particles and crystalline arrays are formed. GdelRNAV display a linear single-stranded RNA genome of ~9 kb, including two open reading frames encoding for replication and structural polyproteins. Phylogenetic relationships based on the RNA-dependent-RNA-polymerase gene marker showed that GdelRNAV are new members of the Bacillarnavirus, a monophyletic genus belonging to the order Picornavirales. GdelRNAV are specific to several strains of G. delicatula. They were rapidly and largely produced (< 12h, 9.34 x 104 virions per host cell). Our analysis points out the host’s variable viral susceptibilities during the early exponential growth phase. Interestingly, we consistently failed to isolate viruses during spring and early summer while G. delicatula developed important blooms. While our study suggests that viruses do contribute to the decline of G. delicatula’s late summer bloom, they may not be the primary mortality agents during the remaining blooms at SOMLIT-Astan. Future studies should focus on the relative contribution of the viral and eukaryotic pathogens to the control of Guinardia’s blooms to understand the fate of these prominent organisms in marine systems.
Till Opatz - One of the best experts on this subject based on the ideXlab platform.
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phytotoxic dioxolanones are potential virulence factors in the infection process of guignardia bidwellii
Scientific Reports, 2017Co-Authors: Iris Buckel, Till Opatz, Lars Andernach, Anja Schuffler, Meike Piepenbring, Eckhard ThinesAbstract:Phytotoxic dioxolanones from Guignardia bidwellii can be described as potential virulence factors which cause the formation of lesions upon an infection by G. bidwellii. The toxin guignardic acid was found in planta of G. bidwellii-infected Vitis vinifera leaves, whereas no phytotoxic dioxolanones were detected in uninfected leaf material. Secondary metabolism analyses of further phytopathogenic fungi from the genus Guignardia led to the observation that all species investigated can produce the phytotoxins known from G. bidwellii. In addition to these studies, it was demonstrated that phenguignardic acid is biosynthetically derived from two molecules of phenylalanine and that phenylalanine is a key precursor in the biosynthesis of the two other phytotoxins – alaguignardic acid and guignardic acid.
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total synthesis of phenguignardic acid a phytotoxic metabolite of guignardia bidwellii
European Journal of Organic Chemistry, 2013Co-Authors: Alexander Stoye, Danuta Kowalczyk, Till OpatzAbstract:(+)-Phenguignardic acid, a phytotoxic metabolite of the grape black rot fungus Guignardia bidwellii was synthesized, as well as its enantiomer, in eight steps from (R)-phenyllactic acid and 3-phenylprop-2-yn-1-ol. The formation of the carboxylate at a late stage, to avoid enolization of the precursor used in the central acetalization step, proved to be crucial. The synthesis of the natural product allowed the unabiguous assignment of its hitherto unknown absolute configuration.
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assignment of configuration in a series of dioxolanone type secondary metabolites from guignardia bidwellii a comparison of vcd and ecd spectroscopy
European Journal of Organic Chemistry, 2013Co-Authors: Lars Andernach, Johannes C Liermann, Iris Buckel, Louis P Sandjo, Eckhard Thines, Till OpatzAbstract:The absolute configurations of a series of phytotoxic dioxolanone-type secondary metabolites isolated from culture filtrates of the grape black rot fungus Guignardia bidwellii were determined by vibrational circular dichroism (VCD) spectroscopy in the mid-IR frequency range. Comparison of the recorded data with DFT calculations showed good agreement between experiment and theory for VCD, whereas electronic circular dichroism (ECD) data for the compounds matched poorly with the predicted spectra obtained by time-dependent DFT (TDDFT) calculations at the same level of theory.
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phytotoxic dioxolanone type secondary metabolites from guignardia bidwellii
Phytochemistry, 2013Co-Authors: Iris Buckel, Daniel Molitor, Johannes C Liermann, Beate Berkelmannlohnertz, Till Opatz, Louis P Sandjo, Eckhard ThinesAbstract:Abstract Phenguignardic acid was recently described as a phytotoxic secondary metabolite from submerged cultures of the grape black rot fungus Guignardia bidwellii. Since the production rate of this natural product in submerged culture is very low, fermentation optimisation was carried out. The optimisation of cultivation conditions led to the identification of seven secondary metabolites, structurally related to guignardic acid, a known secondary metabolite from Guignardia species containing a dioxolanone moiety. All metabolites presented here have not been described to date and are presumably biosynthesised via deamination products of amino acids, such as phenylalanine, valine, tyrosine, and alanine. Four of the seven compounds showed phytotoxic activity. Based on the structures determined by NMR spectroscopy a preliminary structure activity relationship indicated a free carboxyl group as presumably required for the phytotoxic activity.
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phenguignardic acid and guignardic acid phytotoxic secondary metabolites from guignardia bidwellii
Journal of Natural Products, 2012Co-Authors: Daniel Molitor, Johannes C Liermann, Beate Berkelmannlohnertz, Iris Buckel, Till Opatz, Eckhard ThinesAbstract:Bioactivity-guided isolation led to the identification of phenguignardic acid (2), a new phytotoxic secondary metabolite from submerged cultures of grape black rot fungus, Guignardia bidwellii. The compound is structurally related to guignardic acid (1), a dioxolanone moiety-containing metabolite isolated previously from Guignardia species. However, in contrast to guignardic acid, which is presumably synthesized from deamination products of valine and phenylalanine, the biochemical precursor for the biosynthesis of the new phytotoxin appears to be exclusively phenylalanine. Guignardic acid was also found in extracts of cultures from Guignardia bidwellii. The phytotoxic activities of both compounds were assessed in plant assays using either detached vine leaves or intact plants. Antimicrobial and cytotoxic activities of phenguignardic acid were determined.
João Lúcio De Azevedo - One of the best experts on this subject based on the ideXlab platform.
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enzymatic differences between the endophyte guignardia mangiferae botryosphaeriaceae and the citrus pathogen g citricarpa
Genetics and Molecular Research, 2011Co-Authors: Aline Silva Romão, Marcel Bellato Sposito, Fernando Dini Andreote, João Lúcio De Azevedo, Welington Luiz AraujoAbstract:: The endophyte Guignardia mangiferae is closely related to G. citricarpa, the causal agent of citrus black spot; for many years these species had been confused with each other. The development of molecular analytical methods has allowed differentiation of the pathogen G. citricarpa from the endophyte G. mangiferae, but the physiological traits associated with pathogenicity were not described. We examined genetic and enzymatic characteristics of Guignardia spp strains; G. citricarpa produces significantly greater amounts of amylases, endoglucanases and pectinases, compared to G. mangiferae, suggesting that these enzymes could be key in the development of citrus black spot. Principal component analysis revealed pectinase production as the main enzymatic characteristic that distinguishes these Guignardia species. We quantified the activities of pectin lyase, pectin methylesterase and endopolygalacturonase; G. citricarpa and G. mangiferae were found to have significantly different pectin lyase and endopolygalacturonase activities. The pathogen G. citricarpa is more effective in pectin degradation. We concluded that there are significant physiological differences between the species G. citricarpa and G. mangiferae that could be associated with differences in pathogenicity for citrus plants.
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high molecular diversity of the fungus guignardia citricarpa and guignardia mangiferae and new primers for the diagnosis of the citrus black spot
Brazilian Archives of Biology and Technology, 2009Co-Authors: Danyelle Stringari, Chirlei Glienke, Daniel De Christo, Walter Maccheroni, João Lúcio De AzevedoAbstract:RAPD markers were used to investigate the distribution of genetic variability among a group of Guignardia citricarpa, G. mangiferae, and Phyllosticta spinarum isolates obtained from several hosts in Brazil, Argentina, Mexico, Costa Rica, Thailand, Japan, United States and South Africa. Pathogenic isolates G. citricarpa Kiely (anamorph form P. citricarpa McAlp Van Der Aa) are the etiological agent of the Citrus Black Spot (CBS), a disease that affects several citric plants and causes substantial injuries to the appearance of their fruits, thus preventing their export. Several previous studies have demonstrated the existence of an endophytic species with high morphological similarity to the causal agent of CBS that could remain latent in the same hosts. Consequently, the identification of the plants and fruits free from the causal agent of the disease is severely hampered. The RAPD analysis showed a clear discrimination among the pathogenic isolates of G. citricarpa and endophytic isolates (G. mangiferae and P. spinarum). In addition, a Principal Coordinate Analysis (PCO) based on a matrix of genetic similarity estimated by the RAPD markers showed four clusters, irrespective of their host or geographical origin. An Analysis of Molecular Variance (AMOVA) indicated that 62.8% of the genetic variation was found between the populations (G. citricarpa, G. mangiferae, P. spinarum and Phyllosticta sp.). Substantial variation was found in the populations (37.2%). Exclusive RAPD markers of isolates of G. citricarpa were cloned, sequenced and used to obtain SCARS (Sequence Characterized Amplified Regions), which allowed the development of new specific primers for the identification of G. citricarpa PCR (Polymerase Chain Reaction) analysis using a pair of primers specific to pathogenic isolates corroborating the groupings obtained by the RAPD markers, underscoring its efficiency in the identification of the causal agent of CBS.
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fungicide resistance and genetic variability in plant pathogenic strains of guignardia citricarpa
Brazilian Journal of Microbiology, 2009Co-Authors: Vanessa Kavacordeiro, Lygia Vitoria Galliterasawa, Y M Possiede, Juarez Gabardo, João Lúcio De Azevedo, Chirlei GlienkeAbstract:A Mancha Preta dos Citros (MPC) tem ocorrencia mundial afetando a producao de citros na Africa, Oceania e America do Sul. No Brasil, onde o clima e favoravel ao seu desenvolvimento, a doenca esta espalhada nas regioes Sul e Sudeste. O controle da MPC, causada pelo fungo Guignardia citricarpa (anamorfo: Phyllosticta citricarpa) e baseado na aplicacao de fungicidas, como os benzimidazois. Na Africa do Sul, apos 10 anos de controle da doenca com o fungicida benomil, os casos de resistencia a altas concentracoes deste fungicida atingiram todas as areas produtoras. O fungicida estrolilurina chamado azoxistrobina tem se mostrado eficiente no controle dos fitopatogenos de uma grande variedade de culturas economicamente importantes, incluindo a MPC. Neste trabalho foram investigados os efeitos in vitro dos fungicidas benomil e azoxistrobina em 10 linhagens de G. citricarpa isoladas de lesoes em plantas citricas no Brasil e na Africa do Sul. Houve inibicao do crescimento micelial a 0,5 µg/mL do fungicida benomil entre as linhagens testadas, com excecao de PC3C de origem sul-africana, que apresentou resistencia ate a concentracao de 100,0 µg/mL de benomil. A frequencia de mutacao espontânea para resistencia ao benomil foi de 1,25 ´ 10-7. A estrobilurina azoxistrobina, mesmo em altas concentracoes, nao inibiu o crescimento micelial dos isolados, entretanto reduziu significativamente a producao de esporos, chegando a 100% de inibicao em concentracoes de 5,0 µg/mL de azoxistrobina. A variacao na sensibilidade das linhagens, principalmente com a estrobilurina azoxistrobina, possivelmente esta relacionada com a variabilidade genetica dos isolados de G. citricarpa.
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nonpathogenic isolates of the citrus black spot fungus guignardia citricarpa identified as a cosmopolitan endophyte of woody plants g mangiferae phyllosticta capitalensis
Phytopathology, 2002Co-Authors: R. P. Baayen, G C Carroll, I R Van Brouwershaven, P J M Bonants, Walter Maccheroni, G C Schutte, Glienke C De Blanco, Gerard J.m. Verkley, Mathijs De Weerdt, João Lúcio De AzevedoAbstract:ABSTRACT The population structure of Guignardia citricarpa sensu lato (anamorph: Phyllosticta citricarpa), a fungus of which strains pathogenic to citrus are subject to phytosanitary legislation in the European Union and the United States, was investigated. Internal transcribed spacer sequences revealed two phylogenetically distinct groups in G. citricarpa. This distinction was supported by amplified fragment length polymorphism analysis that also supported the exclusion of two isolates that had apparently been misclassified as G. citricarpa. On cherry decoction agar, but not on other media, growth rates of group I isolates were lower than those of group II isolates. Conidial dimensions were similar, but group I isolates formed conidia with barely visible mucoid sheaths, whereas those of group II formed conidia with thick sheaths. Cultures of isolates belonging to group I produced rare infertile perithecia, whereas fertile perithecia were formed by most isolates of group II. Colonies of isolates belonging...
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genetic variability in the endophytic fungus guignardia citricarpa isolated from citrus plants
Genetics and Molecular Biology, 2002Co-Authors: Chirlei Glienkeblanco, Maria Lucia Carneiro Vieira, Paulo Augusto Vianna Barroso, Carlos Ivan Aguilarvildoso, João Lúcio De AzevedoAbstract:During some phases of of their life-cycle endophytic fungi colonize plants asymptomatically being found most frequently inside the aerial part of plant tissues. After surface disinfection of apparently healthy leaves from three varieties of mandarin orange and one tangor, and after incubation on appropriate culture medium, 407 fungal isolates were obtained, giving a total infection frequency of 81%. No fungal growth was observed from disinfected seeds, indicating that fungi are probably not transmitted via seeds. Of the fungal isolates, 27% belonged to the genus Guignardia, with 12 isolates being identified as Guignardia citricarpa Kiely, which is described as a citrus pathogen. The isolates were variable in respect to the presence of sexual structures and growth rates. Most of the isolates produces mature asci, supporting the hypothesis that they are nonpathogenic endophytes, which recently were identified as G. mangiferae. High intraspecific genetic variability (an average similarity coefficient of 0.6) was detected using random amplified polymorphic DNA (RAPD) markers generated by seven different primers. The highest similarity coefficient (0.9) was between isolates P15 and M86 and the smallest (0.22) between isolates P15 and C145. These results did not allow us to establish an association between genetic similarity of the fungal isolates and the citrus varieties from which they were obtained.